Abstract
The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.
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11 December 1998
An Erratum to this paper has been published: https://doi.org/10.1007/BF03651762
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Song, J.M., Inoue, T., Kawazumi, H. et al. Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell. ANAL. SCI. 14, 913–916 (1998). https://doi.org/10.2116/analsci.14.913
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DOI: https://doi.org/10.2116/analsci.14.913