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Resonant X-ray scattering of biological assemblies

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Abstract

Understanding the relationship between structure and function for biological assemblies can guide identification of new therapeutics, design of biomaterials, and development of biotechnological processes. Resonant X-ray scattering provides a chemically-specific approach to characterize complex biological structures based on anomalous or resonant scattering from a specific element or chemical moiety. Anomalous or resonant diffraction can provide structural details with high atomic resolution, while resonant X-ray scattering can provide structural details with lower resolution through tender or soft X-rays. Here, we review applications, challenges, and opportunities for resonant X-ray scattering in the field of structural biology.

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Acknowledgments

This work was supported as part of the Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Basic Energy Sciences under Award No. DE-SC0001090.

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Authors and Affiliations

  1. Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Sintu Rongpipi, Joshua T. Del Mundo, Enrique D. Gomez & Esther W. Gomez

  2. Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Enrique D. Gomez

  3. Materials Research Institute, The Pennsylvania State University, University Park, PA, 16802, USA

    Enrique D. Gomez

  4. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Esther W. Gomez

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Rongpipi, S., Del Mundo, J.T., Gomez, E.D. et al. Resonant X-ray scattering of biological assemblies. MRS Communications 11, 1–17 (2021). https://doi.org/10.1557/s43579-021-00020-4

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