Preparation, characterization, and biological assessment of functionalized reduced graphene oxide–silver nanocomposite

In this work, functionalized reduced graphene oxide–silver (FrGO–Ag) nanocomposite was synthesized to enhance the antimicrobial activity and biocompatibility of FrGO for infected wound burn treatment. The reduction of GO and FGO was confirmed by the removal of some of the oxygen functional groups (carbonyl and epoxy groups) as revealed by Fourier transform infrared (FTIR) spectra. The face-centered cubic (fcc) silver nanoparticles were identified by X-ray diffraction (XRD). The contact time effect and the dose effect of the antimicrobial activity of rGO, FrGO, and FrGO-Ag nanocomposite toward Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans have been investigated. The cytotoxicity results of these compounds revealed that the functionalization by PVP and the decoration by AgNPs improved the biocompatibility of rGO sheets from 35.2 to 88% cell viability against the BJ1 normal human epithelial cell line.


Introduction
Graphene is the thinnest two-dimensional crystalline material that is steady at room temperature. It is a layer of sp 2 -bonded carbon atoms, organized to form honey comb crystal lattice, and is a 0.345 nm thick [1][2][3]. These carbon atoms are arranged in a hexagonal lattice with a C-C distance of 0.142 nm [1,4]. It is considered the basic building block of all graphitic materials; such as fullerenes (zero dimensional (0D), nanotubes (1D), and graphite (3D) [5]. Graphene remarkable properties are reported as high carrier transport mobility as its mobility of charge carriers is ~ 200,000 cm 2 /V/s [6], superior mechanical flexibility with Young's modulus is approximately 1100 GPa and fracture strength is 125 GPa [7], excellent thermal/chemical stability [8], optical transparency [9], high specific surface area with a calculated value of 2630 m 2 /g [10], excellent electrical and thermal conductivity of ~ 5000 W/m/K [11]. Chemical conversion, which involves sonicating flake graphite (Gt) to obtain graphene oxide (GO) and then reducing, allows for low-cost synthesis and processing of bulk-quantity graphene nanosheets, but the use of highly toxic and dangerously unstable NaBH 4 or hydrazine to reduce GO necessitates extreme caution [12]. Another issue is the tendency of products to irreversible aggregation or restacking into Gt due to van der Waals interaction during the chemical reduction [13].
Functionalization has drawn attention since it solved the problem of graphene affinity toward aggregation. The chemical, electrochemical, and sono-chemical methods are examples of graphene functionalization, which is an essential step for preparing FrGO from rGO [14,15]. There are two fundamental types of functionalization; covalent and non-covalent functionalization. Noncovalent interactions are primarily mediated by hydrophobic, van der Waals, and electrostatic forces and necessitate the physical adsorption of appropriate molecules on the graphene surface. Covalent functionalization is considered a structural alteration that can take place at the end of the graphene sheets and/or on their surface. There are four methods for covalent modification of graphene: nucleophilic substitution, electrophilic addition, condensation, and addition [14]. Polyvinylpyrrolidone (PVP) is a biocompatible linear polymer featuring hydrophilic and lipophilic groups in its backbone [16][17][18]. Recently, PVP reduced GO (FrGO) sheets have been studied for different applications, such as electroanalysis [19], microwave absorbing applications [20], and wastewater treatment [21]. In addition, the electrical [16], mechanical [16,22], anticorrosive properties [23], and thermal conductivity [22] have been examined and reported. In wound dressing application, several articles have been reported. Contardi et al. [24] designed electrospun fibrous PVP based hydrogels loaded with hydroxycinnamic acids as potential wound dressings. Contardi et al. [25] fabricated a transparent PVP/hyaluronic acid-based bilayer for the sequential release and delivery of a cutaneous antiseptic and a widely used antibiotic, potentially suitable for wound dressing applications. Paul et al. [26] synthesized biodegradable, stretchable, and transparent plastic films by hybridizing a waterborne aliphatic polyester polyurethane (PU) suspension with PVP. This product has a wide range of applications including agricultural mulches, food and pharmaceutical packaging, and biomedical applications. FrGO has been synthesized by different methods for various applications. Lee et al. [27] used Prussian blue-laden PVP/ reduced pollutants for the removal of hazardous pollutants. Pavinatto et al. [28] prepared PVP/chitosan/rGO electrospun nanofibers to study the ultrasensitive biosensor [28]. Huang et al. [29] prepared PVP-assisted growth of Ni-Co oxide on N-doped rGO to enhance its pseudocapacitive behavior [29]. Li et al. [30] synthesized FeSO 4 /rGO/PVP ternary composite to enhance its microwave absorbing properties [30]. Despite all the reported studies, no one has reported the antimicrobial activity and the cytotoxicity of FrGO (PVP-rGO).
The biological applications of graphene-based materials have been enormously investigated because of their biocompatibility, unique conjugated structure, and relatively low cost [31]. The biological applications of graphene, such as DNA-hybridization device, cancer treatment [32], and delivery of drugs, have also started to be concerned [33]. It also shows a huge potential in other fields of the biological applications such as antibacterial, therapy, and biosensing. Recently, the antimicrobial activities of rGO have been studied. Liu et al. [34] demonstrated the antibacterial activity against Escherichia coli with Gt, graphite oxide (GtO), GO, and reduced GO (rGO). Theirs results showed stronger antibacterial activity than GtO dispersion, and GO revealed the strongest antibacterial activity. The results also showed that antibacterial activities of Gt, GtO, GO, and rGO are concentration-dependent. Gurunathan et al. [35] assessed the P. aeruginosa growth curve with Gt, GtO, GO, and rGO. Their results proved the antibacterial activity of GO and rGO, which demonstrated that the antibacterial activities of graphene-based materials are reliant on the concentration. The antifungal activity was also studied where Sawangphruk et al. [36] showed that rGO totally inhibited the mycelia growth of Aspergillus niger, Aspergillus oryzae, and Fusarium oxysporum at concentrations 500, 500, and 250 µg/ml, respectively.
It has been known that silver nanoparticles (AgNPs) have antimicrobial properties and is widely used as highly efficient antimicrobial agent. In spite of this, AgNPs tends to agglomerate in the process of preparation [37,38]. Several reports have been published on the rGO-Ag antimicrobial activity. For instant, Nguyen et al. [39] reported the antibacterial activity toward E. coli, Listonella anguillarum, Bacillus cereus, and S. aureus bacteria. Han et al. [37] investigated the antimicrobial activity against P. aeruginosa and suggested that RGO/AgNPs exhibited higher antimicrobial activity than AgNPs, owing to the synergic effect of RGO and AgNPs. Deng et al. [40] studied the synthesis of melamine sponge decorated with silver nanoparticles-modified graphene (G/AgNPs-MS) and investigated its antibacterial activity against E. coli and S. aureus bacteria. Fathalipour et al. [41] prepared l-cysteine modified AgNPs-decorated rGO nanocomposite and examined its antibacterial activity against E. coli and S. aureus. Bansala et al. [42] have recently studied the shielding properties of PVP-stabilized-AgNPs-based graphene nanohybrid. Minh Dat et al. [43] have synthesized silver/rGO (Ag/rGO) nanocomposites and have tested it against P. aeruginosa and S. aureus by optical density and plate colony-counting methods. Esmaeili et al. [44] have prepared nanofibrous scaffolds and in order to enhance its antimicrobial activity, rGO/Ag nanostructures were used into the mats. Ghosh et al. [45] fabricated rGO/ Ag decorated conductive cotton fabric for high performing electromagnetic interference shielding and measured the zone of inhibition against E. coli.
To the best of our knowledge, the antimicrobial activity of FrGO and FrGO-Ag nanocomposite, in addition to its cytotoxicity toward dermal cells, has not been reported. This work aims to decrease the cytotoxicity of rGO through functionalization by PVP and to increase its antimicrobial properties by AgNPs decoration. The formulated FrGO-Ag nanocomposite with high antimicrobial activity and low cytotoxicity is meant to be an effective treatment for infected wound burns.

UV-Vis spectroscopy
UV-Vis absorption spectrum [ Fig. 1(A)(a)] of GO shows a characteristic peak at 231 nm and a shoulder at 300 nm, which correspond to π-π* transitions of C-C aromatic bonds and n-π* transitions of C=O bonds, respectively. These results agree with the previously reported data [12,20,21]. After reduction  Figure 1B shows the XRD patterns of rGO (a) and FrGO (b). The pattern of rGO showed two characteristic peaks at 2θ = 23.37° and 42.99°, which are corresponding to d-value 3.8226 A and 2.1038 A, respectively [53]. The disappearance of peak at 11.07°, characteristic of GO sheets besides to the appearance of weak diffraction peak around 23° are evidence of successful reduction of GO sheets through removal of oxygen function groups on the surface of the sheets. Thus, the restacking of rGO sheets was easier to take place. Abulizi et al. [53] reported GO reduction to rGO through the disappearance of diffraction peak at 10.5 (002) and the formation of a new broad peak at around 24 (002) of rGO. While the peak appeared near 43° is due to presence of unexfoliated GtO. These results are in good agreement with reported results in the literature [21,39,54]. Figure 1 Table 1 demonstrate the characteristic wavenumber (cm −1 ) of the main bands and their assignments. The FTIR spectra of GO sheets shows the characteristic peaks at 3368 cm −1 , 1720 cm −1 , 1621 cm −1 , 1384 cm −1 , 1250 cm −1 , and 1067 cm −1 were due to OH stretching, C=O stretching, C=C stretching, OH deformation, epoxy C-O stretching, and alkoxy C-O stretching, respectively [55]. After reduction of GO sheets, the FTIR spectrum of rGO [ Fig. 2

FTIR spectroscopy
shows characteristic peaks at 3438 cm −1 , 2923 cm −1 , and 2854 cm −1 that correspond to OH stretching vibration [56,57], CH 2 symmetric, and asymmetric stretching vibrations, respectively. Moreover, a weak peak at 1722 cm −1 is corresponding to carbonyl (C=O) and a medium peak 1626 cm −1 assigned to carboxylic (C=C) stretching vibrations of the skeletal vibrations of graphitic backbone. In comparison with FTIR spectrum of GO, the intensity of carbonyl (C=O) stretching vibration at 1710 cm −1 decreased and the epoxy (C-O) stretching vibration at 1250 cm −1 disappeared, which confirm the removal of some of the oxygen functional groups of GO sheets. This result is in good agreement with recently reported in literature [58][59][60][61].
The FTIR spectrum of: FGO (a) and FrGO (b) sheets and FrGO-Ag nanocomposite (c) are illustrated in Fig. 2(B). FTIR spectra of FGO sheets [ Fig. 2(B)(a)] shows broad peak at 3433 cm −1 which is ascribed to OH stretching vibration. A weak peak appeared at 1727 cm −1 and a strong peak at 1638 cm −1 which is ascribed to carbonyl (C=O) and carboxylic (C=C) stretching vibrations, respectively. Furthermore, the alkoxy C-O stretching group shifted from 1067 to 1038 cm −1 . These findings confirm the removal of some of the oxygen functional groups of GO sheets. This result is in good agreement with recently reported studies [19,21,62].
Careful examination and comparing all the spectra revealed that FrGO sheets [ Fig. 3    Another shift appeared to lower frequency in alkoxy (C-O) stretching vibration group from 1047 to 1037 cm −1 . A strong peak at 1384 cm −1 , which is assigned to OH deformation of C-OH, showed increase in intensity. This can be explained by attachment of AgNPs to FrGO sheets. On the other side, the peaks at 1725 cm −1 , 1644 cm −1 , 1285 cm −1 , and 1037 cm −1 revealed decrease in intensity. This may be contributed to the reduction of AgNO 3 by using NaBH 4 which reduces carbonyl and epoxy groups causes residual hydroxyl function groups. Yang and colleagues [63] reported that aldehydes and ketones have been frequently reduced to alcohol by NaBH 4 . . The intensity ratio of D to G band (I D /I G ) of FrGO sheets increased from 0.86 to 0.96, which is attributed to the reduction of FGO sheets. These Raman results agree well with those reported by Yoon and In [65]. Stankovich et al. [66] attributed the increase of the intensity ratio (I D /I G ) in Raman spectra of rGO sheets to the decrease in the average size of sp 2 domains due to reduction of the GO. Weng et al. [20] stated that the I D /I G is considered as an indicator to evaluate the size of sp 2 domains and the structural disorder of the graphene nanosheet.

Raman spectroscopy
After decoration of FrGO by AgNPs, the D band and G band of FrGO-Ag nanocomposite [ Fig. 3(B)(c)] shifted to lower frequency from 1348 to 1344 cm −1 and from 1591 to 1584 cm −1 , respectively. In addition, the I D /I G of FrGO-Ag nanocomposite decreased from 0.96 to 0.93 upon the decoration of AgNPs. This result agrees with the reported by Han et al. [37]. It could be observed that an apparent enhancement in Raman signals after the decoration with the AgNPs owing to the plasmon effect of Ag nanoparticles, which is in consistent with Huang et al. [67]. Figure 4(a) representing the HRTEM micrographs of rGO sheets, and FrGO sheets, displayed in Fig. 4(b) show surface modification by PVP. The HRTEM micrographs of FrGO-Ag nanocomposites prepared with different amounts of AgNO 3 solution (10, 30, and 60 μl) for a constant amount of NaBH 4 solution are illustrated in Fig. 4(c-e), respectively. The micrographs represent Ag nanoparticles with small particle sizes, which are freely embedded between FrGO sheets. Figure 4(c) shows a small amount of Ag nanoparticles, while Fig. 4(d, e) show a larger amount of Ag nanoparticles. This owes to the amount of AgNO 3 solution (60 µl) used in the preparation. In addition to some agglomerations of Ag nanoparticles that appeared clearly in Fig. 4(e).

Contact time effect
The antimicrobial activity of rGO, FrGO sheets, FrGO-Ag nanocomposites of fixed concentration (100 µg/ml) is illustrated in Fig. 5(a-c) for different periods of time up to two hours against S. aureus, P. aeruginosa, and C. albicans, respectively.
The rGO sheets showed a sharp decrease in the colony count up to 60 min (around 65%) and reached about 70% after 120 min. This result is found to be in good agreement with those reported in literature. For instance, Gurunathan et al. [35] treated P. aeruginosa with rGO with a constant concentration (75 µg/ml) and found that rGO had a negative effect on bacterial growth, as the turbidity measurements decreased steadily over a 6 h period. After 15 h of exposure to GO, and rGO, there was significant growth inhibition of up to 92%. Akhavan and Ghaderi [68] reported the antibacterial activity of rGO nanowalls (rGONWs) toward E. coli and S. aureus. They found that rGONW exhibited stronger antibacterial activity than their activities against E. coli bacteria. Actually after 1 h, only 5(± 1) % of the S. aureus bacteria was observed on the surface of RGNWs. Although, our results agree with the above-mentioned authors, they disagree with the results reported by Min Peng et al. [69], who reported no significant antimicrobial activity of rGO against oral pathogens namely; C. albicans, Lactobacillus acidophilus, Streptococcus mutans.
The FrGO sheets showed slight antimicrobial activity (around 9%) for almost all the microorganisms. This could be attributed to the functionalization of rGO by PVP, which hindered the oxygen functional group found on the surface of rGO sheets. After decoration of FrGO with AgNPs, apparent inhibition of the colony count occurred, especially for FrGO-Ag nanocomposite which was prepared with high amounts (50 and 60 µl/ml) of AgNO 3 . In Particular, FrGO-Ag nanocomposite prepared with low amounts (10, 20, 30, and 40 µl/ml) of AgNO 3 , showed a gradual decline (range of 58-78%) in the colony count up to 90 min and reach total inhibition after 120 min. On the other hand, FrGO-Ag nanocomposite prepared with high amounts (50 and 60 µl/ml) of AgNO 3 , achieved a maximum loss in colony count of 69% after 60 min and reached total colony loss after 90 min. To sum up, the antimicrobial activity has been enhanced in FrGO-Ag nanocomposite which has been prepared with 60 μl/ml of AgNO 3 due to the decoration FrGO sheets by AgNPs for P. aeruginosa, S. aureus, and C. albicans by nearly 100%, 96%, and 90.5%, respectively.

Dose effect
The minimum dose of inhibition was investigated by using the colony count method for 2 h incubation. The antimicrobial activity of different doses of (25, 50, 75, 100 µg/ml) of rGO, FrGO sheets, and FrGO-Ag nanocomposites (prepared by different amounts of 10, 20, 30, 40, 50, and 60 µl/ml of AgNO 3 ) against S. aureus, P. aeruginosa, and C. albicans was studied and illustrated in Fig. 6(a-c), respectively. Despite the fact that rGO sheets at a concentration of 100 g/ml had a significant effect on colony counts, with a maximum percentage of microorganism killed of approximately 77%, FrGO sheets at the same concentration had only a minor antimicrobial effect, with nearly 8% colony loss. The antibacterial properties of rGO against P. aeruginosa are consistent with the findings of Gurunathan et al. [35] who demonstrated antibacterial properties of rGO against P. aeruginosa incubated for 2 h at 37 °C. They demonstrated that when the cells were exposed to rGO, significant loss was observed at concentrations of 75 µg/ml and 100 µg/ml and maximum loss of P. aeruginosa viability was detected after incubation with 150 µg/ml of rGO.
It could be concluded that decoration of FrGO with AgNPs prepared using high AgNO 3 amounts (50 and 60 µl/ml) significantly improves its antimicrobial activity nearly by 92%, leading to a total loss in colony counts of all studied microorganisms at the dose of 75 μg/ml.

Antimicrobial mechanism
The untreated bacterial (S. aureus) cell and the FrGO-Ag adhered to the treated bacterial (S. aureus) cell are shown in Fig. 7. As shown in Fig. 7(a), untreated S. aureus bacteria show a well-integrated cell wall. Following direct contact with FrGO-Ag, the bacterial cell wall [ Fig. 7(b-d)] appeared to loss its integrity and damage into pieces, followed by the total cellular damage of S. aureus. This irreversible damage could be explained by the direct contact of FrGO-Ag with the bacterial cell, assisting the physical damage through sharp edges. Meanwhile, the AgNPs released into the cytoplasm caused cellular disruption. It could be concluded that the antibacterial mechanism of functionalized graphene-based materials decorated with AgNPs based on the synergistic effect of graphene-based materials and AgNPs. Figure 8 shows the cytotoxic behavior of rGO, FrGO, and FrGO-Ag nanocomposite. After 48 h of incubation with the materials under investigation, cytotoxicity was assessed against the BJ1 normal human epithelial cell line at concentrations ranging from 100 to 0.78 μg/ml. The reduction of GO enhanced its cell viability effect by 10%, where about 35.8% of the cells are viable at rGO sheets against 25.8% at GO. In comparison with the cytotoxicity of FGO, the cell viability of FrGO was reduced (~ 12%) after a reduction from 64.4 to 52.5%. It could be concluded that functionalization of rGO improved the cell viability

Conclusion
It could be concluded from the results that rGO, and FrGO are dose-and time-dependent. The antimicrobial activity of FrGO-Ag nanocomposite prepared with 60 μl/ml of AgNO 3 has been increased owing to the decoration of FrGO sheets by AgNPs, which is nearly 100%, 96%, and 90.5% for P. aeruginosa, S. aureus, and C. albicans, respectively. The reduction of GO sheets reduced the cytotoxicity by about 10%, and the functionalization of rGO sheets by PVP reduced the cytotoxicity by nearly 16.7%. Finally, the biocompatibility of FrGO-Ag nanocomposite against the BJ1 normal human epithelial cell line reached up to 88% cell viability.

Materials
In this work, Synthetic graphite Gt powder was purchased from Sigma-Aldrich, USA, with a particle size of < 20 µm.

Preparation of graphene oxide (GO) sheets
According to the modified Hummers and Offeman's method, GO was prepared from the Gt powder as reported in our previous work [46,47]. NaNO 3 (5 g) was added to H 2 SO 4 (230 ml) in an ice bath, followed by 10 g of Gt (10 g) powder. KMnO 4 was added slowly under violent stirring. Drop by drop, 450 ml of distilled water was added into the flask where the temperature was controlled. Then, 1400 ml of hot distilled water was added under stirring. The reaction was completed by cautious and gentle addition of 50 ml of H 2 O 2 . The resulting Gt was filtered and repeatedly rinsed with dist. water till its pH reached 4.5. The washing process was achieved by centrifugation and dialysis to eliminate the residual acids, metal ions and acids. By ultrasonication of Gt sheets for 30 min, the exfoliated GO sheets were obtained and they were dried at 50 °C.

Preparation of reduced graphene oxide (rGO) sheets
GO solution of concentration (0.01 mg/ml) was dispersed by ultrasound irradiation for 30 min continuously in an ice bath to avoid thermal stress. Ultrasound irradiation was performed by Hummer sonicator. 0.138 g of l-A.A. was added to the dispersed GO under stirring. A few drops of NaOH (1 M) were used to elevate the pH of the medium up to pH 11. The obtained solution was sonicated by performing a pulse mode of 50 s on/50 s off. Sonication occurred at 60 °C for 2 h till the solution turned from a brown color to a brown black color. The obtained sample was filtered immediately and washed several times using deionized water and absolute ethanol to get rid of the excess l-A.A, finally dried at 50 °C.

Preparation of functionalized graphene oxide (FGO) sheets
Using a hummer sonicator, the GO solution of concentration (0.25 mg/ml) was dispersed for 30 min continuously. To control the increase in temperature, the GOsolution was dispersed in ice sonicator. Then, PVP (0.25 g) and l-A.A. (0.5 g) were added. The resulted mixture was incubated at 80 °C for 4 h. Immediately after the incubation, the obtained solution was filtered by sterilized cellulose nitrate membrane filter. The excess dissolved PVP and l-A.A. was removed by repeated washing then dried at 50 °C.

Preparation of functionalized reduced graphene oxide (FrGO) sheets
Dry FGO was dispersed in 1 ml de-ionized water (0.25 mg/ml) for 1 h (pulsed mode: 50 s on/10 s off) by hummer sonicator. 20 µl of NaBH 4 solution (0.1 M) was added under stirring. After 15 min of stirring, the whole solution was filtered and washed with deionized water. The FrGO sheets were obtained by drying the sample at 50 °C.

Preparation of functionalized reduced graphene oxide-silver (FrGO-Ag) nanocomposites
A solution of dispersed FGO (0.05 mg/ml) was prepared and dispersed under a hummer sonicator for 1 h (pulsed mode: 50 s on/10 s off) in an ice bath. Under stirring, 10 µl of AgNO 3 solution was added to the dispersed solution of FGO, and then 20 µl of NaBH 4 (0.1 M) was added to the mixture. With continuous stirring for 15 min, the sample turned from pale black to a yellowish black color. The obtained sample was filtered by sterilized cellulose nitrate membrane filter of 0.45 µ pore size and washed 3 times with de-ionized water. To obtain the functionalized reduced graphene silver (FrGO-Ag) nanocomposite, the obtained sample was dried at 50 °C.

Microorganisms used and preparation
Staphylococcus aureus (ATCC6538), P. aeruginosa (ATCC15442), and C. albicans (ATCC10231) were grown in tryptic soya broth (purchased from Thermo Fisher Scientific Oxoid-UK) at 37 °C during 24 h, their suspensions were homogenized and centrifuged at 3000 rpm for 20 min. The sterile phosphate buffered solution was used in washing three times. The density of each strain inocula was 106 CFU/ml.

Determination of antimicrobial effect
The antimicrobial effect and minimum inhibitory concentration (MIC) of prepared materials were determined against the abovementioned microbial strains [47]. Each one of stock suspension previously prepared was exposed to four concentrations of the materials under investigation (25,50,75, and 100 µg/ml) at three contact times (60, 90, and 120 min).

MTT Cytotoxicity test
The cytotoxicity of composites was reported in our previous work [48]. In brief, for non-radioactive quantification of cell proliferation and cell viability, the cytotoxicity of composites was determined by cell proliferation kit 1 (MTT assay). CO 2 incubator (Sheldon, TC2323, Cornelius, OR, USA) was used to incubate the normal skin fibroblast cell in humidified 5% CO 2 at 37 °C. Various concentrations (from 100 to 0.78 µg/ml) of the composite were added to the cells which were aliquoted in 96-well microliter plastic plates. The cells were then incubated for 48 h. Then, 200 µl of sodium dodecyl sulfate was added to stop the reaction and incubated 12 h at 37 °C. The microplate reader (Bio-Rad Laboratories, model 3350, USA) was used to measure the formazan amount produced at wavelength 595 nm.

Characterization
The UV-Vis absorption spectra were measured in the range between 200 and 800 nm using JASCO V-630 spectrophotometer, Japan. Fourier transform infrared(FTIR) spectra of the prepared samples was recorded by Bruker-VERTEX 70, Germany. The high-resolution transmission electron microscope HRTEM (JEOL Model, JEM-2100, Japan) was used to study the morphological structures of rGO, FrGO, and FrGO-Ag nanocomposite. X-ray diffraction (XRD) patterns were obtained by XRD (X'Pert Pro PANalytical, Holland), targeted by Cu Kα with secondary monochromator (45 kV, 40 mA).

Acknowledgments
This work was funded and supported by Science and Technology Development Fund STDF (Fund Number 12645).

Funding
Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).

Data availability
All data generated or analyzed during this study are included in this published article.

Declarations
Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval
The research carried out within this work did not involve human participants and/or animals.

Open Access
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