Abstract
Transposon-mediated sequencing is an effective method for obtaining full-length high-quality DNA sequence. This method can be applied to the finishing stages of bacterial artificial chromosome (BAC) sequencing, allowing the user to expand a finishing repertoire of custom oligonucleotide sequencing, alternative chemistry sequencing, polymerase chain reaction (PCR), as well as other standard tools for acquiring high-quality data in low-coverage or low-quality regions. In addition to BAC subclones, transposon-mediated sequencing can be applied to cDNA or PCR products subcloned into vectors.
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© 2004 Humana Press Inc., Totowa, NJ
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Reeg, R., Madan, A. (2004). Transposon-Mediated Sequencing. In: Zhao, S., Stodolsky, M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology™, vol 255. Humana Press. https://doi.org/10.1385/1-59259-752-1:197
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DOI: https://doi.org/10.1385/1-59259-752-1:197
Publisher Name: Humana Press
Print ISBN: 978-0-89603-988-9
Online ISBN: 978-1-59259-752-9
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