Abstract
Random oligonucleotide mutagenesis is a method to generate diversity that consists of incorporating random mutations, encoded on a synthetic oligonucleotide, into a specific region of a gene. The number of mutations in individual enzymes within the population can be controlled by varying the length of the target sequence and the degree of randomization during synthesis of the oligonucleotides.
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References
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© 2003 Humana Press Inc., Totowa, NJ
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Sneeden, J.L., Loeb, L.A. (2003). Random Oligonucleotide Mutagenesis. In: Arnold, F.H., Georgiou, G. (eds) Directed Evolution Library Creation. Methods in Molecular Biology™, vol 231. Humana Press. https://doi.org/10.1385/1-59259-395-X:65
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DOI: https://doi.org/10.1385/1-59259-395-X:65
Publisher Name: Humana Press
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Online ISBN: 978-1-59259-395-8
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