Abstract
Hydrogen peroxide formed in the lung tissue in ischemia/reperfusion or released from activated leukocytes causes oxidative injury of the vascular endothelial cells (1–3). H2O2-degrading enzyme, catalase, has been extensively explored in order to protect cells and tissues against H2O2-mediated injury (4). Catalase, however, has short lifetime in the bloodstream and provides only marginal protective effect after intravascular administration in animals (5). Catalase modification (conjugation with polyethylene glycol (6) or encapsulation in liposomes [7]), prolongs catalase lifetime in the circulation and facilitate its cellular uptake. These modifications, however, do not provide it with an affinity to the endothelial cells. In order to provide catalase with such an affinity, catalase could be chemically conjugated with a carrier antibodies recognizing the surface endothelial antigens (8). A monoclonal antibody (MAb) against such an antigen, angiotensin-converting enzyme (anti-ACE MAb 9B9, produced by Dr. Sergei M. Danilov [9]), accumulates in the pulmonary endothelium after systemic injection (10). Therefore, MAb 9B9 may serve as an affinity carrier for targeting of catalase to the pulmonary endothelial cells, for specific augmentation of their antioxidative defense.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fantone, J. C. and Ward, P. A. (1982) Role of oxygen-derived free radicals and metabolites in leukocytes-dependent inflammatory reactions. Am. J. Pathol. 107, 397–424.
Fisher, A. B., Dodia, C., Tan, Z., Ayene, I., and Eckenhoff, R. G. (1991) Oxygendependent lipid peroxidation during lung ischemia. J. Clin. Invest. 88, 674–679.
bSchraufstatter, I., Revack, S., and Cochrane, C. (1984). Proteases and oxidants in experimental pulmonary inflammatory injury. J. Clin. Invest. 73, 1175–1184.
Greenwald, E. (1990) Superoxide dismutase and catalase as therapeutic agents for human diseases. Free Rad. Biol. Med. 8, 201–209.
Turrens, J. F., Crapo, J. D., and Freeman, B. A. (1984) Protection against oxygen toxicity by intravenous injection of liposome-entrapped catalase and superoxide dismutase. J. Clin. Invest. 73, 87–95.
Beckman, J. S., Minor, R. L., White, C. W., Repine, J. E., Rosen, G. M., and Freeman, B. A. (1988) Superoxide dismutase and catalase conjugated with polyethy lene glycole increase endothelial enzyme activity and oxidant resistance. J. Biol. Chem. 263, 6884–6892.
Freeman, B. A., Young, S. L., and Crapo, J. D. (1983) Liposome-mediated augmentation of superoxide dismutase in endothelial cells prevents oxygen injury. J. Biol. Chem. 258, 12,534–12,542.
Sakharov, D. V., Muzykantov, V. R., Domogatsky, S. P., and Danilov, S. M. (1987) Protection of cultured endothelial cells from hydrogen peroxide by antibody-conjugated catalase. Biochim. Biophys. Acta 930, 140–144.
Danilov S., Allikmets, E., Sakharov, I., Dukhanina, E., and Trakht, I. (1987) Monoclonal antibodies against ACE. Biotechnol. Appl. Biochem. 9, 312–322.
Muzykantov V. and Danilov, S. (1995) Targeting of radiolabeled monoclonal antibody against ACE to the pulmonary endothelium, in Targeted Delivery of Imaging Agents (Torchilin, V., ed.), CRC, Boca Ration, FL, pp. 465–485.
Green, M. (1975) Avidin, in Advances in Protein Chemistry (Afunsen, C., Edsall, J., and Richards, F., eds.), Academic, New York, pp. 85–133.
Wilchek, M. and Bayer, E. (1988) The avidin-biotin complex in bioanalytical research. Anal. Biochem. 171, 1–32.
Paganelli, G., Magnani, P., Belloni, C., Siccardi, A., and Fazio, F. (1992) Twostep tumor targeting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin. Eur. J. Nucl. Med. 19, 322–329.
Taylor, R., Sutherland, W., Reist, C., Webb, D. J., Wright, S., and Labunguen, R. (1991) Use of heteropolymeric monoclonal antibodies to attach antigens to the C3b receptor of human erythrocytes: a potential therapeutic treatment. Proc. Natl. Acad. Sci. USA 88, 3305–3309.
Bickel, U., Yoshikawa, T., Landaw, E., Faul, K., and Pardridge, W. (1993) Pharmacological effects in vivo in brain by vector-mediated peptide drug delivery. Proc. Natl. Acad. Sci. USA 90, 2618–2622.
Wu, D., Boado, R., and Pardridge, W. (1996) Complete inactivation of target mRNA by biotinylated antisense oligonucleotide-avidin conjugates. J. Pharm. Exp. Therap. 276, 206–211.
Muzykantov, V., Gavriljuk, V., Reinecke, A., Atochina, E., Kuo, A., Barnathan, E., and Fisher, A. (1995) The functional effects of biotinylation of anti-AEC monoclonal antibody in terms of targeting in vivo. Anal. Biochem. 226, 279–287.
Muzykantov, V., Atochina, E., Gavriljuk, V., Danilov, S., and Fisher, A. (1994) Immunotargeting of streptavidin to the pulmonary endothelium. J. Nucl. Med. 35, 1358–1365.
Muzykantov, V., Atochina, E., Ischiropoulos, H., Danilov, S., and Fisher, A. (1996) Immunotargeting of antioxidant enzymes to the pulmonary endothelium. Proc. Natl. Acad. Sci. USA 93, 5213–5218.
Muzykantov, V. (1997) Conjugation of catalase to a carrier antibody via streptavidin-biotin cross-linker. Biotechnol. Appl. Biochem. 26, 103–109.
Danilov S., Muzykantov, V., Martynov, A., Atochina, E., Sakharov, I., Trakht, I., and Smirnov, V. (1991) Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme. Lab. Invest. 64, 118–124.
Danilov, S., Atochina, E., Hiemish, H., Churakova T., Moldobayeva, A., Sakharov, I., Deichman, G., Ryan, U., and Muzykantov, V. (1994) Interaction of monoclonal antibody to ACE with antigen in vitro and in vivo. Int. Immunol. 6, 1153–1160.
Muzykantov, V., Atochina, E., Granger, D., and Fisher, A. (1997) ICAM-1directed delivery of catalase to the pulmonary vasculature. Am. J. Resp. Crit. Care Med. 155(4), A192.
Atochina, E., Balyasnikova, I., Danilov, S., Granger, D., Fisher, A., and Muzykantov, V. (1998) Immunotargeting of catalase ACE and ICAM-1 protects perfused rat lungs against oxidative stress. Am. J. Physiol. 275, (Lung Cell. Mol. Physiol. 19)L806–L817.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Muzykantov, V.R. (2000). 14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium. In: Francis, G.E., Delgado, C. (eds) Drug Targeting. Methods in Molecular Medicine™, vol 25. Humana Press. https://doi.org/10.1385/1-59259-075-6:241
Download citation
DOI: https://doi.org/10.1385/1-59259-075-6:241
Publisher Name: Humana Press
Print ISBN: 978-0-89603-531-7
Online ISBN: 978-1-59259-075-9
eBook Packages: Springer Protocols