Abstract
Mycoplasma contaminations have a multitude of effects on cultured cell lines that can potentially influence the results of experiments and pollute bioactive substances used in human medicine. The elimination of mycoplasma contaminations in cell cultures has become a practical alternative to discarding and re-establishing important or irreplaceable cell lines. Different quinolones, tetracyclins, and macrolides shown to have strong antimycoplasma properties are employed for the decontamination. We provide detailed descriptions to assure eradication of mycoplasma, to prevent formation of resistant mycoplasma strains, and to cure heavily contaminated and damaged cells. To date, no consistent and permanent alterations that affect the eukaryotic cells during or after the treatment have been detected.
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© 2005 Humana Press Inc., Totowa, NJ
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Uphoff, C.C., Drexler, H.G. (2005). Eradication of Mycoplasma Contaminations. In: Helgason, C.D., Miller, C.L. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 290. Humana Press. https://doi.org/10.1385/1-59259-838-2:025
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DOI: https://doi.org/10.1385/1-59259-838-2:025
Publisher Name: Humana Press
Print ISBN: 978-1-58829-284-1
Online ISBN: 978-1-59259-838-0
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