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NOE Assignment With ARIA 2.0

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 278))

Abstract

The assignment of nuclear Overhauser effect (NOE) resonances is the crucial step in determining the three-dimensional structure of biomolecules from nuclear magnetic resonance (NMR) data. Our program, Ambiguous Retraints for Iterative Assignment (ARIA), treats Noe assignment as an integral part of the structure determination process. This chapter briefly outlines the method and discusses how to carry out a complete structure determination project with the new version 2.0 of ARIA. Two new features greatly streamline the procedure: a new graphical user interface (GUI) and the incorporation of the data model of the Collaborative Computing Project for the NMR community (CCPN). The GUI supports the user in setting up and managing a project. The CCPN data model facilitates data exchange with a great variety of other programs. We give practical guidelines for how to use ARIA and how to analyze results.

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Author information

Authors and Affiliations

  1. Unité de Bio-Informatique Structurale, Institut Pasteur, Paris, France

    Michael Habeck, Wolfgang Rieping, Jens P. Linge & Michael Nilges

Authors
  1. Michael Habeck
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  2. Wolfgang Rieping
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  3. Jens P. Linge
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  4. Michael Nilges
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Oxford, Oxford, UK

    A. Kristina Downing

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© 2004 Humana Press Inc., Totowa, NJ

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Habeck, M., Rieping, W., Linge, J.P., Nilges, M. (2004). NOE Assignment With ARIA 2.0. In: Downing, A.K. (eds) Protein NMR Techniques. Methods in Molecular Biology™, vol 278. Humana Press. https://doi.org/10.1385/1-59259-809-9:379

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  • DOI: https://doi.org/10.1385/1-59259-809-9:379

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-246-9

  • Online ISBN: 978-1-59259-809-0

  • eBook Packages: Springer Protocols

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