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Gene Delivery to Mammalian Cells pp 225–236Cite as

Gene Delivery to the Mouse Brain with Adeno-Associated Virus

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 246))

Abstract

The efficient transduction of postmitotic cells by adeno-associated virus (AAV) makes it an excellent vector to deliver marker, functional, or therapeutic genes to the mammalian brain. An attractive feature of AAV is that all the viral-coding sequences are removed when engineering the recombinant genome, thereby limiting the extent of cell toxicity and immune response that are often associated with viral gene transcription (1).

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Author information

Authors and Affiliations

  1. Department of Pathobiology and Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA

    Marco A. Passini, Deborah J. Watson & John H. Wolfe

  2. Division of Neurology, Children’s Hospital of Philadelphia, Philadelphia, PA

    Marco A. Passini & John H. Wolfe

  3. Department of Neurology, The Children’s Hospital of Philadelphia, Philadelphia, PA

    Deborah J. Watson

Authors
  1. Marco A. Passini
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  2. Deborah J. Watson
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  3. John H. Wolfe
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Editors and Affiliations

  1. Bio-Rad Laboratories, Hercules, CA

    William C. Heiser

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© 2004 Humana Press Inc., Totowa, NJ

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Passini, M.A., Watson, D.J., Wolfe, J.H. (2004). Gene Delivery to the Mouse Brain with Adeno-Associated Virus. In: Heiser, W.C. (eds) Gene Delivery to Mammalian Cells. Methods in Molecular Biology™, vol 246. Humana Press. https://doi.org/10.1385/1-59259-650-9:225

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  • DOI: https://doi.org/10.1385/1-59259-650-9:225

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-095-3

  • Online ISBN: 978-1-59259-650-8

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