Skip to main content

Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases

  • Protocol
Vascular Disease

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 30))

  • 485 Accesses

Abstract

To understand the molecular mechanisms of vascular diseases, studies are designed to process gene function from the discovery of relevant genes to examination of their expression and association with disease. Once a gene has been cloned, Northern blot analysis can directly monitor the gene expression at the mRNA level. Therefore, it is the most expedient strategy for investigating the potential relationship between specific gene expression and disease.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162(1), 156ā€“159.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  2. Chomczynski, P. (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques, 15(3), 532ā€“534.

    CASĀ  PubMedĀ  Google ScholarĀ 

  3. Chirgwin, J. M., et al. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemisrty, 18(24), 5294ā€“5299.

    ArticleĀ  CASĀ  Google ScholarĀ 

  4. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google ScholarĀ 

  5. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., et al., eds. (1993) Current Protocols in Molecular Biology, Vol. 2, Greene Publishing Associates, Inc., Wiley, New York, NY.

    Google ScholarĀ 

  6. Sawamura, T., Kume, N., Aoyam, T., Moriwaki, H., Hoshikawa, H., Aiba, Y., et al. (1997) An endothelial receptor for oxidised low-density lipoprotein. Nature (London) 386, 73ā€“77.

    ArticleĀ  CASĀ  Google ScholarĀ 

  7. Nagase, M., Hirose S., Sawamura T., Masaki T., and Fujita T. (1997) Enhanced expression of endothelial oxidized low-density lipoprotein receptor (LOX-1) in hypertensive rats. Biochem. Biophy. Res. Commun. 237, 496ā€“498.

    ArticleĀ  CASĀ  Google ScholarĀ 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

Ā© 1999 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Chen, M., Sawamura, T. (1999). Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases. In: Baker, A.H. (eds) Vascular Disease. Methods in Molecular Medicineā„¢, vol 30. Humana Press. https://doi.org/10.1385/1-59259-247-3:181

Download citation

  • DOI: https://doi.org/10.1385/1-59259-247-3:181

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-731-1

  • Online ISBN: 978-1-59259-247-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics