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In Situ Hybridization

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 82))

Abstract

In situ hybridization has proven to be a valuable technique for the analysis of gene expression in plants. It allows the investigator to determine the specific cell and tissue types expressing a particular gene. The basic procedure is to hybridize a labeled probe with tissue sections zn sztu. The nonspecifically bound probe is then washed off and the tissue sections are exposed to photographic film for autoradioagraphy.

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© 1998 Human press Inc, Totowa, NJ

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Drews, G.N. (1998). In Situ Hybridization. In: Martinez-Zapater, J.M., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/0-89603-391-0:353

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  • DOI: https://doi.org/10.1385/0-89603-391-0:353

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-391-7

  • Online ISBN: 978-1-59259-268-5

  • eBook Packages: Springer Protocols

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