Abstract
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1.
Measurement of optimum concentration of antigen to coat wells.
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2.
Measurement of optimum dilution of enzyme-linked antibody.
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3.
Use of multichannel- and single-channel micropipets.
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4.
Revision of principles of dilution.
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5.
Makeup and storage of buffers and solutions.
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6.
Learning about observation of tests by eye, and by using multichannel spectrophotometers.
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7.
Handling data.
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8.
Problem solving.
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© 1995 Humana Press Inc.
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Crowther, J.R. (1995). Direct ELISA. In: Crowther, J.R. (eds) ELISA. Methods in Molecular Biology™, vol 42. Humana Press. https://doi.org/10.1385/0-89603-279-5:119
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DOI: https://doi.org/10.1385/0-89603-279-5:119
Publisher Name: Humana Press
Print ISBN: 978-0-89603-279-8
Online ISBN: 978-1-59259-529-7
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