Abstract
Major advances in our understanding of human hematopoiesis have come from the development of semisolid in vitro culture techniques for the detection of progenitor cells capable of colony formation (see Chapter 28, this volume). However, colony assays select for hematopoietic precursors committed to a specific lineage(s) and, therefore, are of limited value in the assessment of very early progenitor cells. Also, they do not provide a means of assessing the influence of microenvironmental cells deemed essential for maintaining hematopoiesis in vivo (1). Furthermore, for the assays to be valid, cells must be plated at a sufficiently low concentration to ensure clonality (a condition in which each colony has arisen from a single progenitor cell). Consequently, cell-cell interactions that may be important in exerting regulatory effects on hematopoiesis are likely to be minimal.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Singer, J. W., Keating, A., and Wright, T. H. (1985) The human hematopoietic micro-environment, in Advances in Haematology, vol. 4 (Hoffbrand, V., ed.) Churchill, London, pp. 1–24.
Dexter, T. M., Allen, T. D., and Lajtha, L. G. (1977) Conditions controlling the proliferation of hematopoietic cells in vitro. J. Cell. Physiol. 91, 335–344.
Gartner, S. and Kaplan, H. S. (1980) Long-term culture of human bone marrow cells. Proc. Natl. Acad. Sci. USA 77, 4756–1759.
Powell, J., Keating, A., Singer, J. W., and Adamson, J. W. (1983) Analysis of hematopoiesis in human long-term marrow cultures. International Society of Experimental Hematology, London, Exp. Hematol. II, 6.
Coulombel, L., Eaves, A. C, and Eaves, C. J. (1983) Enzymatic treatment of long-term marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer. Blood 62, 291–297.
Keating, A., Powell, J., Takahashi, M., and Singer, J. W. (1984) The generation of human long-term marrow cultures from marrow depleted of la (HLA-DR) positive cells. Blood 64, 1159–1162.
Chang, J., Morgenstern, G., Deakin, D., Testa, N. G., Coutinho, L., Scarffe, J. H., Harrison, C., and Dexter, T. M. (1986) Reconstitution of haemopoietic system with autologous marrow taken during relapse of acute myeloblastic leukemia and grown in long-term culture. Lancet 1, 294,295.
Takahashi, M., Keating, A., and Singer, J. W. (1985) A functional defect in irradiated adherent layer from chronic myelogenous leukemia long-term marrow cultures. Exp. Hematol. 13, 926–931.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 The Humana Press Inc.
About this protocol
Cite this protocol
Keating, A., Toor, P. (1990). Human Long-Term Bone Marrow Culture. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:331
Download citation
DOI: https://doi.org/10.1385/0-89603-150-0:331
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
eBook Packages: Springer Protocols