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Vacuum Blotting

An Inexpensive, Flexible, Qualitative Blotting Technique

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New Protein Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 3))

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Abstract

The first system we used to transfer proteins from polyacrylamide gels onto nitrocellulose was a capillary blotting system, based on the work of Renart et al. (1). Although we were very enthusiastic about the wide range of possible applications created by the protein blotting technique, we were unsatisfied with the slow and inefficient transfer. Electroblotting (2,3) seemed to be much more efficient, but at that time there were serious financial limitations in the department.

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References

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© 1988 The Humana Press Inc.

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Peferoen, M. (1988). Vacuum Blotting. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:383

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  • DOI: https://doi.org/10.1385/0-89603-126-8:383

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-126-5

  • Online ISBN: 978-1-59259-490-0

  • eBook Packages: Springer Protocols

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