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Enantiomeric Composition Tests of Ketoprofen in Equine Plasma and Urine as Diastereomeric (S)-(-)-1-Phenylethylamides by Achiral GC–MS

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Abstract

The enantiomeric composition of ketoprofen in equine plasma and urine after administration of a commercial racemic ketoprofen product were determined as diastereomeric (S)-(-)-1-phenylethylamidation by achiral gas chromatography–mass spectrometry in selected ion monitoring mode. This method showed linearity (r ≥ 0.9986) over the range tested (5.0–5,000 ng), with acceptable precision (% RSD ≤ 9.5) and accuracy (% RE = −3.7–7.3). The ratio of (S)-ketoprofen in plasma after 6.0 h and in urine after 71.0 h increased progressively to final values of 67.3 ± 0.1 and 91.9 ± 2.2%, respectively, attributable to the inversion of (R)-ketoprofen to (S)-ketoprofen.

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References

  1. Kim JY, Kim SJ, Paeng KJ, Chung BC (2001) J Vet Pharmacol Ther 24:315–319. doi:10.1046/j.1365-2885.2001.00353.x

    Article  CAS  Google Scholar 

  2. Lombardino JG (1985) Nonsteroidal antiinflammatory drugs. In: Lombardino JG (ed) vol 5.Wiley, New York, pp 303–345

  3. Jamali F, Wainer IW (1993) Drug stereochemistry. In: Jamali F, Wainer IW (eds) Marcel Dekker, New York, pp 375–384

  4. Ghezzi P, Melillo G, Meazza C, Sacco S, Pellegrini L, Asti C et al (1998) J Pharmacol Exp Ther 287:969–974

    CAS  Google Scholar 

  5. Jaussaud P, Bellon D, Besse S, Courtot D, Delatour P (1993) J Vet Pharmacol Ther 18:373–376. doi:10.1111/j.1365-2885.1993.tb00185.x

    Article  Google Scholar 

  6. Verde CR, Simpson MI, Frigoli A, Landoni MF (2001) J Vet Pharmacol Ther 24:179–185. doi:10.1046/j.1365-2885.2001.00336.x

    Article  CAS  Google Scholar 

  7. Stanley SMR, Rodgers JP (1994) In: Proceedings of the 10th international conference of racing analysts and veterinarians, Stockholm, pp 348–349

  8. Short CR, Swan GE, Guthrie AJ, Mulders MSG, Killeen VM, Nurton JP et al (1996) In: Proceedings of the 11th international conference of racing analysts and veterinarians, Queensland, pp 360–363

  9. Presle N, Lapicque F, Gillet P, Hermann M-A, Bannwarth B, Netter P (1998) Eur J Clin Pharmacol 54:351–354. doi:10.1007/s002280050473

    Article  CAS  Google Scholar 

  10. Eichhold TH, Bailey RE, Tanguay SL, Hoke SH (2000) J Mass Spectrom 35:504–511. doi:10.1002/(SICI)1096-9888(200004)35:4<504::AID-JMS958>3.0.CO;2-L

    Google Scholar 

  11. Sallustio BC, Purdie YJ, Whitehead AG, Ahern MJ, Meffin PJ (1988) Br J Clin Pharmacol 26:765–770

    CAS  Google Scholar 

  12. Landoni MF, Lees P (1996) J Vet Pharmacol Ther 19:466–474. doi:10.1111/j.1365-2885.1996.tb00084.x

    Article  CAS  Google Scholar 

  13. Paik MJ, Lee YS, Goto J, Kim KR (2004) J Chromatogr B Analyt Technol Biomed Life Sci 803:257–265. doi:10.1016/j.jchromb.2003.12.026

    Article  CAS  Google Scholar 

  14. Paik MJ, Kim KR (2004) Arch Pharm Res 27:820–824

    Article  CAS  Google Scholar 

  15. Paik MJ, Nguyen DT, Kim KR (2004) Arch Pharm Res 27:1295–1301

    Article  CAS  Google Scholar 

  16. Paik MJ, Nguyen DT, Cho IS, Lee S, Kim KR (2006) Chromatographia 63:623–628. doi:10.1365/s10337-006-0788-8

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was performed with an Agilent 6890N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443–721, Republic of Korea.

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Authors and Affiliations

  1. Racing Laboratory, Korea Racing Authority, Gwacheon, 427-711, Republic of Korea

    J. Yu, M. B. Hu & S. J. Kim

  2. Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Republic of Korea

    J. Yu, Y. Jeong & K. R. Kim

  3. Institute for Neuroregeneration and Stem Cell Research, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea

    G. Lee, Y. H. Ahn & M. J. Paik

  4. Department of Molecular Science and Technology, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea

    G. Lee & M. J. Paik

  5. Institute for Medical Science, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea

    G. Lee

  6. Department of Neurosurgery, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea

    Y. H. Ahn

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  1. J. Yu
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  2. G. Lee
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  3. Y. Jeong
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  4. Y. H. Ahn
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  5. M. B. Hu
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  6. S. J. Kim
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  7. K. R. Kim
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  8. M. J. Paik
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Corresponding authors

Correspondence to K. R. Kim or M. J. Paik.

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Yu, J., Lee, G., Jeong, Y. et al. Enantiomeric Composition Tests of Ketoprofen in Equine Plasma and Urine as Diastereomeric (S)-(-)-1-Phenylethylamides by Achiral GC–MS. Chroma 69, 59–63 (2009). https://doi.org/10.1365/s10337-008-0820-2

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  • DOI: https://doi.org/10.1365/s10337-008-0820-2

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