The Value of Biological and Conditional Factors for Staging of Patients with Resectable Pancreatic Cancer Undergoing Upfront Resection: A Nationwide Analysis

Background Novel definitions suggest that resectability status for pancreatic ductal adenocarcinoma (PDAC) should be assessed beyond anatomical criteria, considering both biological and conditional factors. This has, however, yet to be validated on a nationwide scale. This study evaluated the prognostic value of biological and conditional factors for staging of patients with resectable PDAC. Patients and Methods A nationwide observational cohort study was performed, including all consecutive patients who underwent upfront resection of National Comprehensive Cancer Network resectable PDAC in the Netherlands (2014–2019) with complete information on preoperative carbohydrate antigen (CA) 19-9 and Eastern Cooperative Oncology Group (ECOG) performance status. PDAC was considered biologically unfavorable (RB+) if CA19-9 ≥ 500 U/mL and favorable (RB−) otherwise. ECOG ≥ 2 was considered conditionally unfavorable (RC+) and favorable otherwise (RC−). Overall survival (OS) was assessed using Kaplan–Meier and Cox-proportional hazard analysis, presented as hazard ratios (HRs) with 95% confidence interval (CI). Results Overall, 688 patients were analyzed with a median overall survival (OS) of 20 months (95% CI 19–23). OS was 14 months (95% CI 10 months—median not reached) in 20 RB+C+ patients (3%; HR 1.61, 95% CI 0.86–2.70), 13 months (95% CI 11–15) in 156 RB+C− patients (23%; HR 1.86, 95% CI 1.50–2.31), and 21 months (95% CI 12–41) in 47 RB−C+ patients (7%; HR 1.14, 95% CI 0.80–1.62) compared with 24 months (95% CI 22–27) in 465 patients with RB−C− PDAC (68%; reference). Conclusions Survival after upfront resection of anatomically resectable PDAC is worse in patients with CA19-9 ≥ 500 U/mL, while performance status had no impact. This supports consideration of CA19-9 in preoperative staging of resectable PDAC. Supplementary Information The online version contains supplementary material available at 10.1245/s10434-024-15070-w.

3][4] In contrast to formerly preferred upfront resection followed by adjuvant chemotherapy, neoadjuvant treatment has gained interest over the last decennium.3][14] Internationally, the most commonly used resectability criteria are the National Comprehensive Cancer Network (NCCN) guidelines, which define pancreatic tumors as resectable in case of no arterial contact and ≤ 180° portomesenteric venous tumor contact without vein contour irregularity. 126][17] Biological factors include preoperative serum carbohydrate antigen (CA)19-9 and preoperative regional lymph node metastasis, while the patients' condition is reflected by the Eastern Cooperative Oncology Group (ECOG) performance status. 18ecently, the International Association of Pancreatology (IAP) has proposed to expand the preoperative staging criteria by redefining borderline resectable PDAC with biological and conditional criteria, suggesting that resectability status should be assessed beyond the anatomic relationship between tumor and vessels. 19This has, however, yet to be validated for patients undergoing upfront resection in a nationwide setting.
Therefore, the aim of this study was to evaluate the prognostic value of biological and conditional factors for staging patients with primary resectable pancreatic cancer.

Study Design
A nationwide observational cohort study was performed in all 16 Dutch centers for pancreatic cancer surgery.The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines for reporting observational studies were followed. 20Patients who underwent upfront resection of histologically proven, primary resectable PDAC according to the NCCN criteria between 2014 and 2019 were identified from the mandatory Dutch Pancreatic Cancer Audit (DPCA). 21Patients with an unknown resectability status were excluded.During the study period, the recommended treatment strategy for patients with resectable PDAC was upfront resection.Neoadjuvant treatment was only administered in the context of randomized trials. 5,9herefore, patients who received neoadjuvant treatment were excluded from this study.

Data Collection
Prospective baseline characteristics and perioperative data were retrieved from the audit database after approval of the DPCG scientific committee.Additionally, detailed data on adjuvant therapy, follow-up, and survival were retrieved from patients' medical records.Data on ethnicity and race of patients were not obtained, as these data are not available in the DPCA. 21or each patient, anatomical resectability status was determined retrospectively according to NCCN criteria. 12ubsequently, patients with primary resectable (R) PDAC were categorized on the basis of biological and conditional factors.Patients were considered to have biologically unfavorable PDAC (R B+ ) when preoperative serum CA19-9 level was ≥ 500 U/mL.Patients with serum CA19-9 levels < 500 U/mL were considered R B− .Patients were deemed to have conditionally unfavorable PDAC (R C+ ) if their baseline ECOG performance status was ≥ 2, and patients with an ECOG performance status of 0-1 were classified as having R C− PDAC. 17,19Based on these criteria, patients were stratified into one of four groups, having either (1) R B+C+ PDAC; (2) R B+C− PDAC; (3) R B−C+ PDAC or (4) R B−C− PDAC.The serum CA19-9 level closest to the date of resection was used if multiple preoperative serum CA19-9 samples were available.

Outcomes
The primary outcome was overall survival (OS), defined as the time between the date of tumor resection until the date of death from any cause.The secondary outcome was disease-free survival (DFS), defined as the time between the date of tumor resection until the date of PDAC recurrence diagnosis.PDAC recurrence was pathologically proven, or suspected through imaging, and preferably confirmed by consensus during a multidisciplinary meeting.Alive patients were censored at the date of last follow-up.

Statistical Analysis
Statistical analysis was performed including only patients with complete information on "key variables," i.e., preoperative serum CA19-9 and ECOG performance status.To assess potential selection bias resulting from the complete case analysis, baseline characteristics of included patients were compared with baseline characteristics of patients who were excluded owing to missing data.Other missing baseline data were considered missing at random and imputed based on a Markov Chain Monte Carlo method (five imputations; ten iterations). 22The original cohort and cohort after multiple imputations were compared for inconsistencies.Descriptive statistics were used to compare the prespecified groups.Categorical variables were presented as frequencies and compared using the chi-squared or Fisher's exact test.Parametric continuous variables were shown as mean with standard deviation (SD) and compared using one-way analysis of variance (ANOVA).Non-parametric continuous variables were reported as median with interquartile range (IQR) and compared using the Kruskal-Wallis test.Kaplan-Meier survival curves were used to assess OS and DFS for each group, and univariate Cox proportional hazard analyses were performed to calculate survival differences.Results were presented as hazard ratio (HR) with 95% confidence interval (CI).
Several sensitivity analyses were performed to assess robustness of findings when accounting for underlying factors that may affect the results.4][25] Second, a sensitivity analysis was conducted in a subset of patients with serum CA19-9 levels ≥ 5 U/mL, excluding patients who are considered non or low-secretors of CA19-9. 26,27A third sensitivity analysis was done using a lower CA19-9 threshold for R B+ PDAC, i.e., 200 U/mL, based on the results of a recent study. 16Furthermore, a fourth sensitivity analysis was performed using a lower ECOG threshold, i.e., ≥ 1, to define R C+ PDAC.
Finally, multivariable Cox proportional hazard analyses were conducted to investigate the association between serum CA19-9 and OS, and ECOG performance status and OS, adjusted for potential confounders (i.e., age, sex, tumor size, nodal stage, resection margin status, tumor differentiation, and perineural invasion).
Statistical analyses were performed using R version 3.5.1 (Bell Laboratories, NH, USA) using the "mice" and "survival" packages.A two-tailed P value of ≤ 0.05 was considered statistically significant.

Study Population
Overall, 1906 patients were identified, of whom 1443 underwent upfront resection of NCCN resectable PDAC.Of those, 688 patients were included (Fig. 1).No differences in baseline characteristics were observed in patients who were excluded owing to missing "key variables" compared with included patients (Supplementary Digital Content 1).
A lower serum CA19-9 threshold of ≥ 200 U/mL for defining R B+ resulted in more patients being staged as having R B+C+ PDAC (n = 29, 4%), and R B+C-PDAC (n = 297, 43%).Similar survival differences were found between reclassified groups, with R B+C+ PDAC having the worst median OS of 12 months (95% CI 10-17 months; Fig. 3).A lower threshold for R C+ (ECOG performance status ≥ 1; Supplementary Digital Content 4) resulted in a larger number of patients considered R B+C+ (n = 100; 15%), while the number of patients in the R B-C+ (n = 248; 36%) group also increased.Interestingly, survival was now lowest for patients in the R B+C+ PDAC group, with a median OS of 12 months (95% CI 10-14 months).

DISCUSSION
This study showed that survival after upfront resection is significantly worse for patients with anatomically resectable but biologically unfavorable PDAC (serum CA19-9 ≥ 500 U/mL) than for patients with resectable PDAC and serum CA19-9 < 500 U/mL.ECOG performance status did not impact survival.Similar survival differences were found when applying a lower preoperative serum CA19-9 threshold (≥ 200 U/mL), affecting an even larger group of patients.These findings suggest that serum CA19-9 levels are valuable for preoperative staging of patients with resectable PDAC.
The resectability definition for localized PDAC is traditionally based on vascular tumor involvement.This  definition was introduced to identify patients with LAPC or borderline resectable disease who have a higher risk of R1 resections, in which initial systemic treatment instead of upfront resection is recommended. 28Currently, most guidelines regarding treatment strategies of PDAC focus on anatomical criteria only, although the value of biological and conditional factors is increasingly emphasized. 18,29Considering that accurate preoperative staging of PDAC patients is relevant for guiding treatment strategies and supporting shared-decision making, evaluation of the additional value of these factors is of great importance. 30reviously, two smaller single-center studies were published to validate the IAP proposal, including both biological and conditional factors. 31,32Hayasaki et al. studied 285 patients who received preoperative neoadjuvant chemoradiotherapy, reflecting a different patient group than patients who underwent upfront resection in our study. 32The study by Kato et al. included only 12 conditionally unfavorable patients, impeding a proper analysis of the value of conditional factors. 31The current study is the first to validate the IAP proposal in a large, nationwide cohort of patients who underwent upfront resection.This resulted in a larger number of patients available for analysis in each subgroup, enhancing the power of findings.Moreover, in contrast to previous studies, this study also investigates the interplay of biological and conditional factors, providing a deeper understanding.
An important finding of this study was that, despite having anatomically resectable PDAC, patients with R B+ disease have a dismal prognosis when compared with patients with R B-disease.After correction for potential inaccuracy of CA19-9 owing to hyperbilirubinemia and after exclusion of non or low-secretors of CA19-9, survival differences remained at a similar disadvantage for the R B+ PDAC group.2][33][34][35][36][37] One study determined CA19-9 ≥ 200 U/mL to be associated with worse survival outcomes after PDAC resection, while another study established the threshold of CA19-9 at 1000 U/mL. 16,31Considering that a lower cutoff for CA19-9 may impact a higher number of patients, a sensitivity analysis was performed with a less strict threshold FIG. 2 Kaplan-Meier curves and results from Cox proportional hazard analysis comparing overall survival between patients with R B+C+ , R B+C− , R B−C+ , and R B−C− PDAC; R B+ was defined as preoperative serum CA19-9 levels ≥ 500 U/ mL and R B− as CA19-9 < 500 U/mL; R C+ was considered with an ECOG performance status ≥ 2 and R C− with ECOG 0-1

Strata
of CA19-9 ≥ 200 U/mL for R B+ .Survival outcomes after upfront resection for the larger group of R B+ patients with a serum CA 19-9 ≥ 200 U/mL were significantly worse compared with the other PDAC groups.Therefore, a preoperative serum CA19-9 threshold of ≥ 200 U/mL may be considered for preoperative staging of resectable PDAC.
In contrast to the widely studied importance of preoperative serum CA19-9, less is known about the need to incorporate conditional factors for preoperative staging of resectable PDAC.Conditional factors might be important, as they are negatively associated with complications after surgery, refraining from chemotherapy, and poor survival. 17,38revious studies reported ECOG performance status to be a major prognostic factor for survival in patients with PDAC. 17,31,32,38Moreover, other factors reflecting conditional status have also been associated with survival, such as radiomics and body composition measures on preoperative imaging. 39,40Nevertheless, only the ECOG performance status was included in proposed staging criteria.In our study, however, survival in patients with R B-C+ PDAC was similar to survival in R B-C-patients.For the small group of patients with R B+C+ , survival outcomes seemed mainly disadvantaged because of an unfavorable tumor biology.Furthermore, ECOG performance status was not associated with decreased OS in a multivariable model.Nevertheless, patients with a poor performance status might have been determined eligible for surgery only after careful selection during multidisciplinary team meetings.These meetings have been initiated to screen patients on frailty and surgical risk, herewith improving patient selection while also paying attention to prehabilitation to improve patient fitness before surgery. 41Consequently, failure to demonstrate a difference in survival between R B-C+ patients and R B-C-might be a result of confounding by indication.
The addition of biological and conditional parameters for preoperative staging has been proposed previously and usually contained elevated preoperative serum CA19-9. 18,29,42he IAP consensus statement regarding novel borderline resectability criteria also considered patients with preoperative regional lymph nodes metastasis, diagnosed by positron emission tomography-computed tomography (PET-CT) or nodal biopsy, to have borderline resectable disease. 19The value of preoperative regional lymph node metastasis as an expression of biologically unfavorable PDAC could not be assessed in this study since PET-CT and nodal biopsy were not performed routinely.Interestingly, however, higher rates FIG. 3 Sensitivity analysis: Kaplan-Meier curves and Coxproportional hazard analysis comparing overall survival between patients with R B+C+ , R B+C− , R B−C+ , and R B−C− PDAC, defining R B+ as CA19-9 ≥ 200 U/mL; R B+ was defined as preoperative serum CA19-9 levels ≥ 200 U/mL and R B− as CA19-9 < 200 U/mL; R C+ was considered with an ECOG performance status ≥ 2 and R C− with ECOG 0-1 of positive pathological lymph nodes were found for patients with R B+ .Evaluation of the additive value of biopsy or PET-CT to assess preoperative regional lymph node metastasis could therefore be a potential focus of future studies.
Currently, guidelines recommend administration of neoadjuvant chemo(radio)therapy for anatomically borderline resectable PDAC, supported by the results of recent randomized controlled trials. 43These studies showed better OS after neoadjuvant treatment in borderline resectable patients compared with upfront surgery.Since R B+ patients had a dismal prognosis after upfront tumor resection in our study, neoadjuvant treatment with intensive chemotherapeutic regimens, such as FOLFORINOX, could be suggested for this group.This has already been implemented in clinical practice in some largevolume pancreatic expert centers. 18However, to prove that this is the optimal treatment strategy for these patients, evidence from randomized controlled trials must be obtained.
This study has several limitations.First, the study population consisted only of patients who underwent upfront PDAC resection.Patients initially scheduled for pancreatic resection, but who refrained from resection owing to fast progressive disease and health deterioration, were not included.However, the results of this study are still applicable to the vast majority of patients, since approximately 75% of patients scheduled for surgery eventually undergo PDAC resection. 5,44,45Second, a complete case analysis based on completeness of "key variables" was performed to allow for a more accurate evaluation of the different staging categories.As a result, patients with unknown preoperative serum CA19-9 or ECOG performance status were excluded, causing potential selection bias.However, comparison of characteristics between included and excluded patients revealed no difference.Moreover, the R C+ groups were quite small, so nonsignificant differences may be due to insufficient power.Consequently, the findings with regard to the impact of ECOG performance status as a conditional factor should be interpreted with care.Finally, the current study only included patients with resectable PDAC, as the recommended treatment strategy for borderline resectable PDAC now consists of neoadjuvant therapy instead of upfront resection.Nevertheless, it would be valuable to further explore the impact of biological and conditional factors in patients with borderline resectable PDAC and in the context of neoadjuvant treatment as well, as this could aid in refining patient stratification.
In conclusion, this nationwide observational cohort study demonstrated that patients with anatomically resectable but biologically unfavorable PDAC (defined as a preoperative serum CA19-9 level ≥ 500 U/mL) have worse survival than patients with preoperative serum CA19-9 < 500 U/mL, independent of the patients' performance status.The inclusion of CA19-9 for preoperative staging of patients with resectable PDAC should be considered, although prospective studies will need to determine whether neoadjuvant treatment is beneficial for these patients.

TABLE 1
Baseline characteristics of 688 patients undergoing resection of anatomical resectable PDAC according to the predefined subgroups a B+ was defined as preoperative serum CA19-9 levels ≥ 500 U/mL, and B− as preoperative serum CA19-9 levels < 500 U/mL; C+ was defined as an Eastern Cooperative Oncology Group performance status ≥ 2, and C− as Eastern Cooperative Oncology Group performance status 0-1 b Major complications were defined as complications requiring a surgical or radiological intervention, intensive care unit admittance, organ failure, or death AJCC American Joint Committee on Cancer, ASA American Society of Anesthesiologists, BMI body mass index, ECOG Eastern Cooperative Oncology Group, IQR interquartile range, PDAC pancreatic ductal adenocarcinoma