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The Melanogenesis-Inhibitory Effect and the Percutaneous Formulation of Ginsenoside Rb1

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Abstract

Ginsenoside Rb1 (Rb1) is the most predominant ginsenoside isolated from the roots of ginseng (Panax ginseng C. A. Meyer). This compound is active in various human biological pathways that are involved in human collagen synthesis and inhibition of cell apoptosis. In this study, the skin-whitening effects of Rb1 were investigated in B16 melanoma cells. Our results showed that Rb1 inhibited melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16 cells in a dose-dependent manner, which collectively indicated that Rb1 may have skin-whitening effects and may be formulated into skin-whitening products for skin care. Accordingly, a ginsenoside collagen transdermal patch was developed as a vehicle to topically deliver Rb1 into pig skin. The percutaneous permeation, retention within skin, and release in vitro of Rb1 from seven transdermal patch formulas were studied. It was determined that the best formula for ginsenoside collagen transdermal patch is made of protein collagen hydrolysate powder (PCHP) 2.0% (w/w), methyl cellulose (MC) 0.5% (w/w), polyethyleneglycol 6000 (PEG6000) 0.5% (w/w), ginsenoside 0.036% (w/w), azone 0.4% (v/w), menthol 0.20% (w/w), and water.

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Acknowledgments

This work was supported by Jiangsu Overseas Research and Training Program for University Prominent Young and Middle-aged Teachers and Presidents sponsored by Jiangsu Provincial Department of Education, and also by Hong Kong Baptist University FRG2/08-09/089. The authors also would like to greatly thank Mulin Yang for his proof reading and English correction and Dr. Yu Hua and Yuen Tsz-kin from the Center for Cancer and Inflammation Research of Hong Kong Baptist University for their patient technical guidance in B16 cell culture test.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowlong Tong, Kowloon, Hong Kong, China

    Lin Wang, Ai-Ping Lu, Zhi-Ling Yu, Zhao-Xiang Bian, Li-Min Zhou, HuBiao Chen, Min Xu & Zhijun Yang

  2. College of Pharmacy, Suzhou Health College, Suzhou, 215009, China

    Lin Wang

  3. Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong, China

    Ricky N. S. Wong, Hoi-Hin Kwok & Patrick Ying-Kit Yue

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  1. Lin Wang
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  2. Ai-Ping Lu
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  11. Zhijun Yang
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Correspondence to Zhijun Yang.

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Wang, L., Lu, AP., Yu, ZL. et al. The Melanogenesis-Inhibitory Effect and the Percutaneous Formulation of Ginsenoside Rb1. AAPS PharmSciTech 15, 1252–1262 (2014). https://doi.org/10.1208/s12249-014-0138-3

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  • DOI: https://doi.org/10.1208/s12249-014-0138-3

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