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Expression of Fox Head Protein 1 in Human Eutopic Endometrium and Endometriosis

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Abstract

The objective of this study is to examine the localization and expression of FOXP1 in human endometrium during the menstrual cycle and in endometriotic lesions and endometrial adenocarcinoma. FOXP1 protein expression was analyzed by immunohistochemistry and Western blot. FOXP1 expression was significantly different between glandular epithelial and stromal nuclei and cytoplasm in both endometrial functionalis and basalis. FOXP1 immunostaining was significantly reduced in the early secretory stage in comparison to the mid proliferative stage in the functionalis and the early proliferative stage in the basalis. FOXP1 expression was found in endometriotic lesions but not in endometrial adenocarcinoma. Multiple protein bands of FOXP1 were identified, and their presence varied considerably among patients. Protein expression levels were significantly higher in the mid and late secretory stages in comparison to early proliferative and early secretory stages. FOXP1 protein is present in human endometrium with evidence of dependent changes in expression.

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Authors and Affiliations

  1. Centre for Women’s Health Research, Monash University Department of Obstetrics and Gynaecology, 246 Clayton Road, Clayton, Victoria, 3168, Australia

    Lulu Fu MB, MSc, Jane E. Girling BSc Hons, PhD & Peter A. W. Rogers BSc Hons, PhD

  2. Monash Medical Centre, Monash Institute of Medical Research, Clayton, Victoria, Australia

    Lulu Fu MB, MSc, Jane E. Girling BSc Hons, PhD & Peter A. W. Rogers BSc Hons, PhD

Authors
  1. Lulu Fu MB, MSc
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  2. Jane E. Girling BSc Hons, PhD
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  3. Peter A. W. Rogers BSc Hons, PhD
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Corresponding author

Correspondence to Jane E. Girling BSc Hons, PhD.

Additional information

Ms Fu was supported by the Australian Postgraduate Award. Dr Girling’s salary was provided by NH & MRC grant 384195. Dr Rogers’s salary was provided by NH & MRC fellowship grant 143805.We thank Leonie Cann and Dr Anna Ponnampalam for their technical assistance with immunohistochemistry and Western blotting, respectively.We also thank clinical research nurses Nancy Taylor, Nicki Sam, and Pam Mamers and the surgeons for tissue collection and the patients of Monash Medical Centre for their participation.

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Fu, L., Girling, J.E. & Rogers, P.A.W. Expression of Fox Head Protein 1 in Human Eutopic Endometrium and Endometriosis. Reprod. Sci. 15, 243–252 (2008). https://doi.org/10.1177/1933719107312626

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  • DOI: https://doi.org/10.1177/1933719107312626

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