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Cytoskeletal turnover and Myosin contractility drive cell autonomous oscillations in a model of Drosophila Dorsal Closure

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Abstract

Oscillatory behaviour in force-generating systems is a pervasive phenomenon in cell biology. In this work, we investigate how oscillations in the actomyosin cytoskeleton drive cell shape changes during the process of Dorsal Closure (DC), a morphogenetic event in Drosophila embryo development whereby epidermal continuity is generated through the pulsatile apical area reduction of cells constituting the amnioserosa (AS) tissue. We present a theoretical model of AS cell dynamics by which the oscillatory behaviour arises due to a coupling between active myosin-driven forces, actin turnover and cell deformation. Oscillations in our model are cell-autonomous and are modulated by neighbour coupling, and our model accurately reproduces the oscillatory dynamics of AS cells and their amplitude and frequency evolution. A key prediction arising from our model is that the rate of actin turnover and Myosin contractile force must increase during DC in order to reproduce the decrease in amplitude and period of cell area oscillations observed in vivo. This prediction opens up new ways to think about the molecular underpinnings of AS cell oscillations and their link to net tissue contraction and suggests the form of future experimental measurements.

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Authors and Affiliations

  1. Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK

    P. F. Machado

  2. Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK

    G. B. Blanchard

  3. Centro de Biología Molecular Severo Ochoa, CSIC, C/ Nicolás Cabrera 1, 28049, Madrid, Spain

    J. Duque & N. Gorfinkiel

Authors
  1. P. F. Machado
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  2. G. B. Blanchard
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  3. J. Duque
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  4. N. Gorfinkiel
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Correspondence to P. F. Machado.

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Machado, P.F., Blanchard, G.B., Duque, J. et al. Cytoskeletal turnover and Myosin contractility drive cell autonomous oscillations in a model of Drosophila Dorsal Closure. Eur. Phys. J. Spec. Top. 223, 1391–1402 (2014). https://doi.org/10.1140/epjst/e2014-02197-7

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  • DOI: https://doi.org/10.1140/epjst/e2014-02197-7

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