Abstract
In order to elucidate the role of proteins CLV1, CLV2, CLV3, and WUS in the mechanism underlying the maintenance of compartmental structure (spatial arrangement of the zones of biosynthesis of marker proteins) of the shoot apical meristem, a model of such mechanism was developed. Computational experiments led to biologically plausible solutions only when synthesis of substance W in a space between the organizing center and meristem apex was limited by the mechanism based on interaction of CLV3 with membrane receptor CLV1/CLV2 and lower boundary of the zone of W synthesis was determined by isoline of the corresponding threshold level of substance Y concentration. The model of the “reaction-diffusion” type formalizing the role proteins CLV1/CLV2, CLV3, and WUS can describe the basis of the mechanism underlying regulation of the compartmental structure of the shoot apical meristem and positioning of the organizing center in a certain site of the cell ensemble of such meristem.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashe, H.L. and Briscoe, J., The Interpretation of Morphogen Gradients, Development, 2006, vol. 133, pp. 385–394.
Clark, S.E., Organ Formation at the Vegetative Shoot Meristem, Plant Cell, 1997, vol. 9, pp. 1067–1076.
Clark, S.E., Running, M.P., and Meyerowitz, E.M., CLAVATA3 Is a Specific Regulator of Shoot and Floral Meristem Development Affecting the Same Processes as CLAVATA, Development, 1995, vol. 121, pp. 2057–2067.
Laux, T., Mayer, K.F.X., Berger, J., and Jurgens, G., The WUSCHEL Gene Is Required for Shoot and Floral Meristem Integrity in Arabidopsis, Development., 1996, vol. 122, pp. 87–96.
Lenhard, M. and Laux, T., Stem Cell Homeostasis in the Arabidopsis Shoot Meristem Is Regulated by Intercellular Movement of CLAVATA3 and Its Sequestration by CLAVATA1, Development, 2003, vol. 130, pp. 3163–3173.
Meicenheimer, R.D., Relationships between Shoot Growth and Changing Phyllotaxy of Ranunculus, Am. J. Botan., 1979, vol. 66, pp. 557–569.
Nikolaev, S.V., Kolchanov, N.A., Fadeev, S.I., et al., A Study of One-Dimensional Model of Regulation of Restorative Zone Sizes in Biological Tissue, Vychislit. Tekhnologii, 2006, vol. 11, no. 2, pp. 67–81.
Nikolaev, S.V., Fadeev, S.I., Penenko, A.V., et al., Systemic Approach to Studying Morphogenesis in Arabidopsis thaliana. 2. Modeling of Regulation of the Shoot Meristem Structure, Biofizika, 2007, ((in press).
Reddy, G.V. and Meyerowitz, E.M., Stem-Cell Homeostasis and Growth Dynamics Can Be Uncoupled in the Arabidopsis Shoot Apex, Science, 2005, vol. 310, pp. 663–667.
Reinhardt, D., Frenz, M., Mandel, T., and Kuhlemeier, C., Microsurgical and Laser Ablation Analysis of Interactions between the Zones and Layers of the Tomato Shoot Apical Meristem, Development, 2003, vol. 130, pp. 4073–4083.
Rojo, E., Sharma, V.K., Kovaleva, V., et al., CLV3 Is Localized to the Extracellular Space, Where It Activates the Arabidopsis CLAVATA Stem Cell Signaling Pathway, Plant Cell, 2002, vol. 14, pp. 969–977.
Sharma, V.K., Carles, C., and Fletcher, J.C., Maintenance of Stem Cell Populations in Plants, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 11823–11829.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S.V. Nikolaev, A.V. Penenko, V.V. Lavreha, E.D. Mjolsness, N.A. Kolchanov, 2007, published in Ontogenez, 2007, Vol. 38, No. 6, pp. 457–462.
Rights and permissions
About this article
Cite this article
Nikolaev, S.V., Penenko, A.V., Lavreha, V.V. et al. A model study of the role of proteins CLV1, CLV2, CLV3, and WUS in regulation of the structure of the shoot apical meristem. Russ J Dev Biol 38, 383–388 (2007). https://doi.org/10.1134/S1062360407060069
Received:
Issue Date:
DOI: https://doi.org/10.1134/S1062360407060069