Skip to main content
SpringerLink
Log in

Molecular cloning of the GhLOM and GhROX genes associated with axillary meristem formation in cotton

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Axillary meristem (AM) is the prerequisite of shoot branch development, which then determines plant aerial architecture. Many transcription factors are known to influence AM initiation. In this study, we isolated two genes from cotton (Gossypium hirsutum L.): GhLOM and GhROX. Sequence analysis showed that GhLOM was a GRAS protein and GhROX was a bHLH transcription factor. They shared conserved protein domains with LOM and ROX, whose mutants showed the AM defect in Arabidopsis thaliana. Phylogenetic analysis of protein sequences found GhLOM in the same group as LOM-like proteins closely related to PtHAM and SlHAM, while GhROX was clustered with ROX. Expression analysis was performed by RTPCR in different plant organs and revealed that both genes predominantly accumulated in apices. The study of subcellular localization demonstrated that GhLOM and GhROX were mainly concentrated in nucleus. Transactivation assay was performed and showed that both genes were transcriptionally active.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AM:

axillary meristem(s)

ORFs:

open reading frames

SAM:

shoot apical meristem

References

  1. McSteen, P. and Leyser, O., Shoot branching, Annu. Rev. Plant Biol., 2005, vol. 56, pp. 353–374.

    Article  CAS  PubMed  Google Scholar 

  2. Snow, M. and Snow, R., The determination of axillary buds, New Phytol., 1942, vol. 41, pp. 13–22.

    Article  Google Scholar 

  3. Garrison, R., Studies in the development of axillary buds, Am. J. Bot., 1955, vol. 42, pp. 257–266.

    Article  Google Scholar 

  4. Bennett, T. and Leyser, O., Something on the side: axillary meristems and plant development, Plant Mol. Biol., 2006, vol. 60, pp. 843–854.

    Article  CAS  PubMed  Google Scholar 

  5. Cline, M.G., Concepts and terminology of apical dominance, Am. J. Bot., 1997, vol. 84, pp. 1064–1069.

    Article  CAS  PubMed  Google Scholar 

  6. Napoli, C.A., Beveridge, C.A., and Snowden, K.C., Reevaluating concepts of apical dominance and the control of axillary bud outgrowth, Curr. Top. Dev. Biol., 1999, vol. 44, pp. 127–169.

    Article  CAS  PubMed  Google Scholar 

  7. Gomez-Roldan, V., Fermas, S., Brewer, P.B., Puech-Pagès, V., Dun, E.A., Pillot, J.P., Letisse, F., Matusova, R., Danoun, S., and Portais, J.C., Strigolactone inhibition of shoot branching, Nature, 2008, vol. 455, pp. 189–194.

    Article  CAS  PubMed  Google Scholar 

  8. Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., and Yamaquchi, S., Inhibition of shoot branching by new terpenoid plant hormones, Nature, 2008, vol. 455, pp. 195–200.

    Article  CAS  PubMed  Google Scholar 

  9. Kebrom, T.H., Spielmeyer, W., and Finnegan, E.J., Grasses provide new insights into regulation of shoot branching, Trends Plant Sci., 2013, vol. 18, pp. 41–48.

    Article  CAS  PubMed  Google Scholar 

  10. Janssen, B.J., Drummond, R.S.M., and Snowden, K.C., Regulation of axillary shoot development, Curr. Opin. Plant Biol., 2014, vol. 17, pp. 28–35.

    Article  PubMed  Google Scholar 

  11. Waldie, T., McCulloch, H., and Leyser, O., Strigolactones and the control of plant development: lessons from shoot branching, Plant J., 2014, vol. 79, pp. 607–622.

    Article  CAS  PubMed  Google Scholar 

  12. McSteen, P., Hormonal regulation of branching in grasses, Plant Physiol., 2009, vol. 149, pp. 46–55.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Schumacher, K., Schmitt, T., Rossberg, M., Schmitz, G., and Theres, K., The Lateral suppressor (Ls) gene of tomato encodes a new member of the VHIID protein family, Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 290–295.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Schmitz, G., Tillmann, E., Carriero, F., Fiore, C., Cellini, F., and Theres, K., The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 1064–1069.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Greb, T., Clarenz, O., Schafer, E., Muller, D., Herrero, R., Schmitz, G., and Theres, K., Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation, Genes Dev., 2003, vol. 17, pp. 1175–1187.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Keller, T., Abbott, J., Moritz, T., and Doerner, P., Arabidopsis REGULATOR OF AXILLARY MERISTEMS1 controls a leaf axil stem cell niche and modulates vegetative development, Plant Cell, 2006, vol. 18, pp. 598–611.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Muller, D., Schmitz, G., and Theres, K., Blind homologous R2R3 Myb genes control the pattern of lateral meristem initiation in Arabidopsis, Plant Cell, 2006, vol. 18, pp. 586–597.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Yang, F., Wang, Q., Schmitz, G., Müller, D., and Theres, K., The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis, Plant J., 2012, vol. 71, pp. 61–70.

    Article  CAS  PubMed  Google Scholar 

  19. Li, X., Qian, Q., Fu, Z., Wang, Y., Xiong, G., Zeng, D., Wang, X., Liu, X., Teng, S., Hiroshi, F., Yuan, M., Luo, D., Han, B., and Li, J., Control of tillering in rice, Nature, 2003, vol. 422, pp. 618–621.

    Article  CAS  PubMed  Google Scholar 

  20. Komatsu, M., Maekawa, M., Shimamoto, K., and Kyozuka, J., The LAX1 and FRIZZY PANICLE 2 genes determine the inflorescence architecture of rice by controlling rachis-branch and spikelet development, Dev. Biol., 2001, vol. 231, pp. 364–373.

    Article  CAS  PubMed  Google Scholar 

  21. Komatsu, K., Maekawa, M., Ujiie, S., Satake, Y., Furutani, I., Okamoto, H., Shimamoto, K., and Kyozuka, J., LAX and SPA: major regulators of shoot branching in rice, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 11765–11770.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Tabuchi, H., Zhang, Y., Hattori, S., Omae, M., Shimizu-Sato, S., Oikawa, T., Qian, Q., Nishimura, M., Kitano, H., Xie, H., Fang, X., Yoshida, H., Kyozuka, J., Chen, F., and Sato, Y., LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems, Plant Cell, 2011, vol. 23, pp. 3276–3287.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Ritter, M.K., Padilla, C.M., and Schmidt, R.J., The maize mutant barren stalkl is defective in axillary meristem development, Am. J. Bot., 2002, vol. 89, pp. 203–210.

    Article  PubMed  Google Scholar 

  24. McConnell, J.R. and Barton, M.K., Effect of mutations in the PINHEAD gene of Arabidopsis on the formation of shoot apical meristems, Dev. Genet., 1995, vol. 16, pp. 358–366.

    Article  Google Scholar 

  25. Talbert, P.B., Adler, H.T., Parks, D.W., and Comai, L., The REVOLUTA gene is necessary for apical meristem development and for limiting cell divisions in the leaves and stems of Arabidopsis thaliana, Development, 1995, vol. 121, pp. 2723–2735.

    CAS  PubMed  Google Scholar 

  26. Otsuga, D., de Guzman, B., Prigge, M.J., Drews, G.N., and Clark, S.E., REVOLUTA regulates meristem initiation at lateral positions, Plant J., 2001, vol. 25, pp. 223–236.

    Article  CAS  PubMed  Google Scholar 

  27. Hibara, K., Karim, M.R., Takada, S., Taoka, K., Furutani, M., Aida, M., and Tasaka, M., Arabidopsis CUPSHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation, Plant Cell, 2006, vol. 18, pp. 2946–2957.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Schulze, S., Schäfer, B.N., Parizotto, E.A., Voinnet, O., and Theres, K., LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems, Plant J., 2010, vol. 64, pp. 668–678.

    Article  CAS  PubMed  Google Scholar 

  29. Wang, L., Mai, Y.X., Zhang, Y.C., Luo, Q., and Yang, H.Q., MicroRNA171c-targeted SCL6-II, SCL6-III, and SCL6-IV genes regulate shoot branching in Arabidopsis, Mol. Plant, 2010, vol. 3, pp. 794–806.

    Article  PubMed  Google Scholar 

  30. Czechowski, T., Stitt, M., Altmann, T., Udvardi, M.K., and Scheible, W.R., Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis, Plant Physiol., 2005, vol. 139, pp. 5–17.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    T. C. Dai & Z. M. Wang

Authors
  1. T. C. Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. M. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z. M. Wang.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dai, T.C., Wang, Z.M. Molecular cloning of the GhLOM and GhROX genes associated with axillary meristem formation in cotton. Russ J Plant Physiol 63, 375–382 (2016). https://doi.org/10.1134/S1021443716030031

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443716030031

Keywords

Search

Navigation