Abstract
By the hot water extraction method, galactomannan was extracted (4.5% yield of the seed mass) from the seeds of Chinese honey locust (Gleditsia sinensis Lam). It had a molecular weight of 1230 kDa, and its solutions had a high viscosity [η] of 1064 ml/g and optical activity [α]D of +21.4°. The polysaccharide consists of mannose and galactose residues in the molar ratio 2.69: 1. In the galactomannan macromolecule the backbone is formed by 1,4-β-D-mannopyranose residues, 37% of which are substituted by α-D-galactopyranose at C6. By 13C-NMR-spectroscopy, fragments of differently galactose-substituted mannobiose units were found to be in the galactomannan being studied: Man-Man, (Gal)Man-Man, and Man-Man(Gal) in the ratio of 0.23: 0.47: 0.30.
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Original Russian Text © D.N. Oleynikov, A.V. Rohin, 2010, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2010, Vol. 46, No. 1, pp. 113–118.
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Oleynikov, D.N., Rohin, A.V. Galactomannan from the seeds of chinese honey locust (Gleditsia sinensis Lam.). Appl Biochem Microbiol 46, 103–107 (2010). https://doi.org/10.1134/S0003683810010187
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DOI: https://doi.org/10.1134/S0003683810010187