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Supramolecular Complexes of Tetrapeptides Capable of Inducing the Human α-Lactalbumin β-Domain Conformational Transitions

  • STRUCTURE OF MACROMOLECULAR COMPOUNDS
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Abstract

It is shown using small-angle X-ray scattering (SAXS) that the tetrapeptide, which is an inductor of fibrillogenesis for a fragment of α-human lactalbumin β-domain, is active in the form of supramolecular complexes. Formation of these complexes was not detected by microscopy and analytical gel filtration. Molecular-dynamics simulation of the behavior of an ensemble of tetrapeptides using the free diffusion method confirmed their experimentally observed tendency to form oligomers. The obtained data and methodical approaches can be applied to develop new peptide drugs, which can modulate the activity of proteins by affecting their spatial structure.

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ACKNOWLEDGMENTS

The SAXS curves were obtained at the European Synchrotron Radiation Facility (Grenoble, France), experiment no. LS2508 @ID02.

Funding

This study was supported by the National Research Centre “Kurchatov Institute” (order no. 1363, dated June 25, 2019).

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Authors and Affiliations

  1. Konstantinov Petersburg Nuclear Physics Institute of National Research Centre “Kurchatov Institute”, 188300, Gatchina, Russia

    Ya. A. Zabrodskaya, A. V. Shvetsov, Yu. P. Garmay, D. V. Lebedev & V. V. Egorov

  2. Peter the Great St. Petersburg Polytechnic University, 195251, St. Petersburg, Russia

    Ya. A. Zabrodskaya & A. V. Shvetsov

  3. Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, 197376, St. Petersburg, Russia

    Ya. A. Zabrodskaya

  4. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia

    Ya. A. Zabrodskaya, A. V. Shvetsov, D. V. Lebedev & V. V. Egorov

  5. European Synchrotron Radiation Facility, CS 40220, 38043, Grenoble, France

    R. Dattani

  6. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    V. V. Egorov

Authors
  1. Ya. A. Zabrodskaya
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  2. A. V. Shvetsov
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  3. Yu. P. Garmay
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  4. D. V. Lebedev
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  5. R. Dattani
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  6. V. V. Egorov
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Corresponding author

Correspondence to V. V. Egorov.

Additional information

Translated by Yu. Sin’kov

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Zabrodskaya, Y.A., Shvetsov, A.V., Garmay, Y.P. et al. Supramolecular Complexes of Tetrapeptides Capable of Inducing the Human α-Lactalbumin β-Domain Conformational Transitions. Crystallogr. Rep. 66, 840–845 (2021). https://doi.org/10.1134/S1063774521050254

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