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Current Status of Male Fertility Preservation in Humans

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Abstract

Fertility preservation is often needed in assisted reproduction and to preserve fertility of patients of reproductive age with spermatogenic failure, severe oligozoospermia, a history of ejaculatory or collection issues, or for those at risk of iatrogenic sterilization from medical procedures or treatments such as vasectomy, testicular surgery, radiation therapy and chemotherapy. This review focuses on the current status of different approaches and technologies in male fertility preservation including cryopreservation of sperm, testicular tissue, and male germline stem cells. The cryobiological characteristics of sperm cells and testicular tissue as well as the mechanisms of cryoinjuries are also discussed.

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Funding

This work was supported by the National Natural Science Foundation of China (the Joint Funds of NSFC and Henan Province U1604179), Henan Province Foundation (20B310013), Xinxiang City Foundation (GG2019022), Fertility Preservation Technology Innovation Team of Sanquan College of Xinxiang Medical University (SQTD201801) and Key Teacher Training Project of Sanquan College of Xinxiang Medical University (SQ2019GGJS06).

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  1. Key Laboratory of Fertility Preservation, Sanquan College of Xinxiang Medical University, 453003, Xinxiang, China

    Huanhuan Hu, Guojie Ji, Xiaowei Shi, Jing Zhang & Mingwen Li

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  1. Huanhuan Hu
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  2. Guojie Ji
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  3. Xiaowei Shi
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  4. Jing Zhang
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ABBREVIATIONS

SSC, spermatogonial stem cell; CPA, cryoprotective agents; IUI, intra-uterine insemination; IVF, in vitro fertilization; ICSI, intracytoplasmic sperm injection; IVS, in vitro spermatogenesis; TESA, testicular sperm aspiration; DMSO, dimethyl sulfoxide; EG, ethylene glycol; ROS, reactive oxygen species; GSH, reduced glutathione; MTG, monothioglycerol.

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Correspondence to Mingwen Li.

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Huanhuan Hu, Ji, G., Shi, X. et al. Current Status of Male Fertility Preservation in Humans. Russ J Dev Biol 53, 134–140 (2022). https://doi.org/10.1134/S1062360422020060

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