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In-silico Evaluation of Structurally Homologous Drosophila p53 with Human p53 to Identify Functional Differences for Future Therapeutic Research

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Abstract

p53 is a transcription factor, which is encoded by the TP53 gene in human. p53 regulates the cell cycle and functions as a tumour suppressor, thus, involved in preventing cancer. In vivo, analysis of p53 mechanisms in whole animal models are critical, alternatively Drosophila can be selected. Our present study worked on comparative and interactome profiling of p53 protein using PyMol and Haddock 2.4. The structural similarity resulted that p53 of Drosophila (Dmp53) is almost alike to that of human p53 (Hp53) protein. Hp53 interacting proteins were systematically docked and analyzed with Hp53 and Dmp53 both. Furthermore the associated complexes are analysed through Discovery Studio. The interactome study revealed better homologous interaction like human p53. Drosophila p53 exhibits characteristics that makes it a promising model for cancer research, bearing resemblance to human p53.

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AVAILABILITY OF DATA AND MATERIALS

The data that supports the findings of this study are available in the supplementary material of this article.

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ACKNOWLEDGMENTS

This work was supported by Oriental Institute of Science and Technology in the frame of our Research Programme.

Funding

This work was supported by ongoing institutional funding. No additional grants to carry out or direct this particular research were obtained.

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Authors and Affiliations

  1. Swami Vivekananda University, Barrackpore, North 24 Parganas, West Bengal, India

    Nutan Tudu

  2. Post Graduate Department of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, 721102, Midnapore, West Bengal, India

    Aniket Sarkar & Bidyut Bandyopadhyay

  3. Post Graduate Department of Biochemistry, Oriental Institute of Science and Technology, Vidyasagar University, 721102, Midnapore, West Bengal, India

    Sayan Mondal & Bidyut Bandyopadhyay

  4. Post Graduate Department of Biotechnology and Biochemistry, Molecular Informatics Laboratory, Oriental Institute of Science and Technology, Vidyasagar University, 721102, Midnapore, West Bengal, India

    Anindya Sundar Panja

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  1. Nutan Tudu
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Contributions

The Study and Method development by A.S.P and B.B, material preparation, analyzing data by N.T, experiments performed A.S and S.M, interpreted the results B.B and A.S.P and manuscript is written by A.S.P.

Corresponding author

Correspondence to Anindya Sundar Panja.

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This article does not contain studies with human or animal subjects performed by any of the authors that should be approved by Ethics Committee.

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Supplementary Information

10525_2024_9407_MOESM1_ESM.pdf

Supplementary Figs. 1–10. Representation of Bond interactome involving ATM, BCL2, CDK2, CDKN1, CHEK2, EP300, MDM2, MDM4, CREB and TP73 with Human p53 and Drosophila p53.

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Nutan Tudu, Sarkar, A., Mondal, S. et al. In-silico Evaluation of Structurally Homologous Drosophila p53 with Human p53 to Identify Functional Differences for Future Therapeutic Research. Biol Bull Russ Acad Sci 50 (Suppl 3), S310–S316 (2023). https://doi.org/10.1134/S1062359021101738

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