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Comparative Analysis of the HAP2/GCS1, GEX2 Gene Expression in Maize Lines of Saratov Selection

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Abstract

The haploid induction phenomenon has scientific and practical aspects for development of effective haploid-inducing maize line and creation of diploid homozygous lines. The article discusses the relationship between the maize haploid induction and the disturbance of gamete interactions, in particular, for GEX2-HAP2/GCS1-mediated adhesion and fusion of gamete membranes in Zarodyshevyi Marker Saratovskii Purpurnyi (ZMSP) maize line. Using real-time RT-PCR, it was found that the GEX2 and HAP2/GCS1 genes are expressed not only in sperm but also in ovules; however, the maize haploid-inducing ability does not correlate with their expression. It was shown that the ZMSP haploid-inducing line has 27 SNPs, one 9-bp insertion, and one 2-bp polymorphism in the GEX2 gene and the corresponding ten amino acid substitutions with two insertions in the GEX2 protein in comparison with the B73 reference line. These substitutions, possibly, affect the protein conformation and the interaction of gamete membranes. It was found that HAP2/GCS1, a more evolutionarily conserved protein for gamete membrane fusion in maize, has only one amino acid substitution in ZMSP in comparison with B73 and Korichnevyi Marker maize lines.

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ACKNOWLEDGMENTS

We are grateful to O.I. Yudakova for reading the manuscript and valuable comments, V.V. Fadeev for help in preparation of the manuscript, and the reviewer for useful comments.

Funding

This work was supported by the Russian Foundation for Basic Research (project nos. 20-016-00020a and 20-316-80020/20mol-e), a grant from the President of the Russian Federation (no. MK-4527.2022.1.4, Ministry of Education and Science), and the Basic Research Program of the State Academies of Sciences for 2021–2023 (no. 121031700141-7).

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Subdivision of the Federal Research Center, Saratov Scientific Centre of the Russian Academy of Sciences, 410049, Saratov, Russia

    E. M. Moiseeva, Y. S. Gusev & M. I. Chumakov

  2. Chernyshevsky Saratov National Research State University, 410012, Saratov, Russia

    Y. S. Gusev & O. V. Gutorova

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  1. E. M. Moiseeva
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  2. Y. S. Gusev
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  3. O. V. Gutorova
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  4. M. I. Chumakov
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Correspondence to M. I. Chumakov.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Moiseeva, E.M., Gusev, Y.S., Gutorova, O.V. et al. Comparative Analysis of the HAP2/GCS1, GEX2 Gene Expression in Maize Lines of Saratov Selection. Russ J Genet 59, 281–288 (2023). https://doi.org/10.1134/S1022795423030092

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