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Effects of UV-B Irradiation Alone and in Combination with Salicylic Acid on the Growth and Active Ingredients of Dendrobium officinale

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Abstract

The quality of Dendrobium officinale Kimura et Migo in cultivation was worse than those collected in wild, this work was conducted to explore a method that can improve the quality of D. officinale in cultivation. Seedlings were sprayed with different concentrations of SA with UV-B irradiation (15.6 µW/cm2) for 4 h per day. After 8 days, partial growth indicators and active ingredients in the seedlings were determined. The results showed that UV-B irradiation alone could increase the contents of malondialdehyde (MDA), total polysaccharides, total alkaloids and total flavonoids as well as the activities of antioxidant enzymes, and decrease the contents of chlorophyll in D. officinale. Moreover, UV-B irradiation combined with SA could significantly increase the contents of total polysaccharides, total flavonoids, and chlorophyll as well as the activities of antioxidant enzymes, and decreased the contents of MDA and total alkaloids of D. officinale, when compared with UV-B alone. Importantly, the growth and main active ingredients of D. officinale were enhanced when the seedlings were treated with various concentrations (1.0–3.0 mM) of SA and grown under intense UV-B irradiation. At the optimal concentration (2.5 mM) of SA, the content of polysaccharides was further increased by 27.82%, while the content of MDA was decreased by 43.51%, when compared with UV-B alone. This study provides an insight for improving the quality of D. officinale bionic cultivated in its habitat, which might promote its industrialization.

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Funding

This work was supported by Guangdong Science and Technology Department, China under Grant (project nos. 2016A040402020 and 2017A020108006).

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    Authors and Affiliations

    1. School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China

      X. Y. Feng, D. D. Cui, L. J. Zeng, Z. X. Wu, X. X. Xie, J. S. Zhang & J. L. Huang

    2. Key Laboratory of State Administration of Traditional Chinese Medicine for Production and Development of Cantonese Medicinal Materials, Guangzhou, China

      X. Y. Feng, L. J. Zeng, Z. X. Wu, X. X. Xie, J. S. Zhang & J. L. Huang

    3. Shaoguan Institute of High-Tech Industry, South China University of Technology, Shaoguan, China

      D. D. Cui & X. Y. Zhang

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    1. X. Y. Feng
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    Authors X.Y. Feng and D.D. Cui contributed equally to this work and should be considered co-first authors.

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    Correspondence to L. J. Zeng.

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    Statement on the welfare of humans or animals. This article does not contain any studies involving animals performed by any of the authors.

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    Abbreviations: CAT—catalase; CK—control group; MDA— malondialdehyde; POD—peroxidase; ROS—reactive oxygen species; SA—salicylic acid; SOD—superoxide dismutase; UV— ultraviolet.

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    Feng, X.Y., Cui, D.D., Zeng, L.J. et al. Effects of UV-B Irradiation Alone and in Combination with Salicylic Acid on the Growth and Active Ingredients of Dendrobium officinale . Russ J Plant Physiol 68, 483–490 (2021). https://doi.org/10.1134/S1021443721030043

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