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Effect of Spinal Cord Injury on P2 Signaling in the Cholinergic Synapse

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Abstract

Thoracic spinal cord injuries are well known to entail the degradation of spinal motoneurons, accompanied by axonal degeneration. In the present study, the functional integrity of neuromuscular transmission was assessed via stimulation mechanomiography in Wistar rats. We demonstrated a decrease in the modulating activity of ATP in the cholinergic synapse due to spinal cord injury (a model of spinal cord contusion injury) vs. hypodynamia (a model of anti-orthostatic hindlimb suspension). The revealed abnormal purine-mediated modulation of the neuromuscular junction provides evidence for axonal degeneration and suggests that trans-synaptic degeneration of motor neurons may occur below the spinal cord injury level in patients with similar traumas.

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Funding

This work was supported by the grant 2/22-5 to Kazan State Medical University, Ministry of Health Care of Russia, for the implementation of scientific research within the University Development Program and as part of the Strategic Academic Leadership of Kazan Federal University (PRIORITY-2030).

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Authors and Affiliations

  1. Kazan State Medical University, Kazan, Russia

    A. E. Khairullin, D. V. Efimova, S. N. Grishin & A. U. Ziganshin

  2. Kazan Federal University, Kazan, Russia

    A. E. Khairullin, A. A. Eremeev & D. E. Sabirova

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  1. A. E. Khairullin
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  2. D. V. Efimova
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  3. A. A. Eremeev
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  4. D. E. Sabirova
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  5. S. N. Grishin
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  6. A. U. Ziganshin
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All authors equally contributed to conceptualization and design of the study, as well as data collection and processing, writing and editing the manuscript.

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Correspondence to A. E. Khairullin.

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COMPLIANCE WITH ETHICAL STANDARDS

The experiments were carried out in compliance with current ethical standards. All applicable international, national and/or institutional principles of animal care and use were observed. All the procedures that involved laboratory animals met the ethical standards approved by the legal acts of the Russian Federation and principles of the Basel Declaration.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 5, pp. 588–599https://doi.org/10.31857/S0869813923050059.

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Khairullin, A.E., Efimova, D.V., Eremeev, A.A. et al. Effect of Spinal Cord Injury on P2 Signaling in the Cholinergic Synapse. J Evol Biochem Phys 59, 822–830 (2023). https://doi.org/10.1134/S0022093023030158

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