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Superoxide Dismutase and Catalase Activities in Tissues of the Black Sea Bivalve Mollusks Cerastoderma glaucum (Bruguière, 1789), Anadara kagoshimensis (Tokunaga, 1906) and Mytilus galloprovincialis Lam. as Related to Adaptation to Their Habitats

  • Comparative and Ontogenic Biochemistry
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Abstract

Tissue- and species-specific features of superoxide dismutase (SOD) and catalase activity, as well as levels of thiobarbituric acid reactive substances (TBARS), were investigated in the hepatopancreas, gills and foot of the three Black Sea bivalve mollusks—lagoon cockle Cerastoderma glaucum, mussel Mytilus galloprovincialis and blood clam Anadara kagoshimensis—sharing high natural oxidative stress tolerance. Cockle tissues displayed a far lower TBARS level compared to that in the mussel and blood clam, with values increasing in the following sequence: cockle–clam–mussel. Of the three bivalve species, a highest activity of both antioxidant enzymes was detected in all cockle tissues. The blood clam and lagoon cockle, as burrowing mollusks, shared a similar tissue specificity and displayed a highest activity of both enzymes in the gills. The blood clam was appreciably superior to the mussel as to SOD and catalase activity in the gills but inferior in terms of SOD activity in the hepatopancreas and foot. The revealed features reflect a different oxidative stress tolerance of the bivalves studied in their natural habitats. A highest oxidative stress sensitivity was found in the mussel. By contrast, the lagoon cockle was distinguished by a highest oxidative stress tolerance, while the blood clam occupied an intermediate position. These species-specific features enable mollusks to successfully adapt to oxidative stress which typically occurs in benthic biotopes of the Black Sea.

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ACKNOWLEDGMENTS

We are sincerely grateful to A.V. Borodina and A.Yu. Gostyukhin for the aid in collecting mollusks and to N.V. Nikolsky for his consultation on statistics.

Funding

This work was implemented within a state assignment to A.O. Kovalevsky Institute of Biology of the Southern Seas (Sevastopol) (АААА-А18-118021490093-4 of 14.02.2018 and АААА-А19-119060690014-5 of 06.06.2019).

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  1. A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Sevastopol, Russia

    O. L. Gostuykhina & T. I. Andreenko

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  1. O. L. Gostuykhina
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Correspondence to O. L. Gostuykhina.

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Gostuykhina, O.L., Andreenko, T.I. Superoxide Dismutase and Catalase Activities in Tissues of the Black Sea Bivalve Mollusks Cerastoderma glaucum (Bruguière, 1789), Anadara kagoshimensis (Tokunaga, 1906) and Mytilus galloprovincialis Lam. as Related to Adaptation to Their Habitats. J Evol Biochem Phys 56, 113–124 (2020). https://doi.org/10.1134/S0022093020020039

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