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Photodynamic therapy and two-photon bio-imaging applications of hydrophobic chromophores through amphiphilic polymer delivery

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Abstract

The synthesis and photophysical properties of two lipophilic quadrupolar chromophores featuring anthracenyl (1) or dibromobenzene (2) were described. These two chromophores combined significant two-photon absorption cross-sections with high fluorescence quantum yield for 1 and improved singlet oxygen generation efficiency for 2, in organic solvents. The use of Pluronic nanoparticles allowed a simple and straightforward introduction of these lipophilic chromophores into biological cell media. Their internal distribution in various cell lines was studied using fluorescence microscopy and flow-cytometry following a successful staining that was achieved upon 2 h of incubation. Finally, multiphoton excitation microscopy and photodynamic therapy capability of the chromophores were demonstrated by cell exposure to a 820 nm fs laser and cell death upon one photon resonant irradiation at 436 ± 10 nm, respectively.

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Authors and Affiliations

  1. Université de Lyon, CNRS UMR 5182, Institut de Chimie de Lyon, Université Lyon 1, Ecole Normale Supérieure de Lyon, Site Monod, 46 allée d’Italie, 69364, Lyon Cedex 07, France

    Thibault Gallavardin, Yann Bretonnière, Mikael Lindgren, Frédéric Lerouge, Stéphane Parola, Olivier Maury & Chantal Andraud

  2. Laboratoire de Spectrométrie Physique Université Joseph Fourier, CNRS - UMR 5588, BP 87 F-38402, Saint Martin d’Hères, France

    Mathieu Maurin, Timea Simon, Ana-Maria Gabudean, Patrick L. Baldeck & Olivier Stéphan

  3. INSERM, U851, UniversitéLyonl, IFR128, 21 AvenueTony Garnier, Lyon, F-69007, France

    Sophie Marotte, Yann Leverrier & Jacqueline Marvel

  4. Department of Physics, Norwegian University of Science and Technology, 7491, Trondheim, Norway

    Mikael Lindgren

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  1. Thibault Gallavardin
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  2. Mathieu Maurin
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  3. Sophie Marotte
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Correspondence to Chantal Andraud.

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Gallavardin, T., Maurin, M., Marotte, S. et al. Photodynamic therapy and two-photon bio-imaging applications of hydrophobic chromophores through amphiphilic polymer delivery. Photochem Photobiol Sci 10, 1216–1225 (2011). https://doi.org/10.1039/c0pp00381f

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