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Laser-assisted fluorescence microscopy for measuring cell membrane dynamics

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Abstract

Membranes of living cells are characterized by laser-assisted fluorescence microscopy, in particular a combination of microspectrofluorometry, total internal reflection fluorescence microscopy (TIRFM), fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) spectroscopy. The generalized polarization (GP, characterizing a spectral shift which depends on the phase of membrane lipids) as well as the effective fluorescence lifetime (teff) of the membrane marker laurdan were revealed to be appropriate parameters for membrane stiffness and fluidity. GP decreased with temperature, but increased during cell growth and was always higher for the plasma membrane than for intracellular membranes. Microdomains of different fluorescence lifetimes teff were observed at temperatures above 30 °C and disappeared during cell aging. Non-radiative energy transfer was used to detect laurdan selectively in close proximity to a molecular acceptor (DiI) and may present a possibility for measuring membrane dynamics in specific microenvironments.

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Authors and Affiliations

  1. Fachhochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430, Aalen, Germany

    Herbert Schneckenburger, Michael Wagner & Martina Kretzschmar

  2. Institut für Lasertechnologien in der Medizin und Messtechnik an der Universität Ulm, Helmholtzstr. 12, 89081, Ulm, Germany

    Herbert Schneckenburger, Wolfgang S. L. Strauss & Reinhard Sailer

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  1. Herbert Schneckenburger
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  2. Michael Wagner
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  3. Martina Kretzschmar
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  4. Wolfgang S. L. Strauss
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  5. Reinhard Sailer
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Schneckenburger, H., Wagner, M., Kretzschmar, M. et al. Laser-assisted fluorescence microscopy for measuring cell membrane dynamics. Photochem Photobiol Sci 3, 817–822 (2004). https://doi.org/10.1039/b317047k

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