Skip to main content

Advertisement

SpringerLink
Log in

Intermittent exposure to fluorescent light extends lifespan of human diploid fibroblasts in culture

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

SHORT exposure (3 or 4 h) to fluorescent light one to three times weekly enhances the proliferation rate of human diploid fibroblasts in culture1. This proliferative response is reversible and is mediated through the culture medium, Dulbecco Vogt's supplemented with 10% foetal bovine serum. Conceivably, this increase in proliferation rate might also influence the lifespan of human diploid fibroblasts which undergo a limited number of population doublings (PDs) in culture2. To evaluate the long term effects of repeated light exposure on lifespan, three lines of human fibroblasts originating from adult skin (HU-274), a mince of whole foetus (HU-278), and foetal lung (WI-38), were assayed in quantitative experiments to determine the lifespan measured as cumulative cell number and total PDs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Parshad, R. & Sanford, K. K. J. cell. Physiol. (in the press).

  2. Hayflick, L. Expl Cell Res. 37, 614–636 (1965).

    Article  CAS  Google Scholar 

  3. Ryan, J. M., Sharf, B. B. & Cristofalo, V. J. Expl Cell Res. 91, 389–392 (1975).

    Article  CAS  Google Scholar 

  4. Macieira-Coelho, A. Experientia 22, 390 (1966). Cristofalo, V. J. Adv. geront. Res. 4, 45–79 (1972). Cristofalo, V. J. Adv. exp. Med. Biol. 61, 57–79 (1975)

    Article  CAS  Google Scholar 

  5. Wang, R. J. In Vitro 12, 19–22 (1976). Pereira, O. M., Smith, J. R., & Packer, L., Photochem. Photobiol. 24, 237–242 (1976).

    Article  CAS  Google Scholar 

  6. Litwin, J. Expl Geront. 7, 381–386 (1972).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, College of Medicine, Howard University, Washington DC, 20059

    RAM PARSHAD

  2. Biochemistry Laboratory, National Cancer Institute, National Institutes Health, US Public Health Service, Bethesda, Maryland, 20014

    KATHERINE K. SANFORD

Authors
  1. RAM PARSHAD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. KATHERINE K. SANFORD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

PARSHAD, R., SANFORD, K. Intermittent exposure to fluorescent light extends lifespan of human diploid fibroblasts in culture. Nature 268, 736–737 (1977). https://doi.org/10.1038/268736a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/268736a0

  • Springer Nature Limited

This article is cited by

Search

Navigation