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Inhibition of the Phosphofructokinase Reaction in Perfused Rat Heart by Respiration of Ketone Bodies, Fatty Acids and Pyruvate

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Abstract

IN rat heart muscle the conversion of fructose-6-phosphate to fructose-1 : 6-diphosphate (catalysed by phosphofructokinase) is not capable of significant reversal (because of the extremely low fructose-1 : 6-diphosphatase activity of this tissue1). Conclusions may therefore be drawn concerning the activity of the phosphofructokinase reaction from measurements of the concentrations of glucose-6-phosphate, fructose-6-phosphate and fructose-1 : 6-diphosphate. An increase in the diphosphate concentration in association with a fall in the concentrations of monophosphates is interpreted as reflecting an increase in the activity of the phosphofructokinase reaction and vice versa1,2. In this way it has been shown that anoxia activates the phosphofructokinase reaction and that starvation and alloxan-diabetes lead to its inhibition2,3. Since in starvation and diabetes the availability to muscle of ketone bodies and fatty acids is increased it seemed important to investigate the effects of these substrates on hexose phosphate concentrations in the perfused rat heart.

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References

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Author notes

  1. K. L. MANCHESTER: Mr. and Mrs. John Jaffé Donation Student of the Royal Society.

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge,

    E. A. NEWSHOLME, P. J. RANDLE & K. L. MANCHESTER

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  1. E. A. NEWSHOLME
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  2. P. J. RANDLE
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  3. K. L. MANCHESTER
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NEWSHOLME, E., RANDLE, P. & MANCHESTER, K. Inhibition of the Phosphofructokinase Reaction in Perfused Rat Heart by Respiration of Ketone Bodies, Fatty Acids and Pyruvate. Nature 193, 270–271 (1962). https://doi.org/10.1038/193270a0

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