Skip to main content
SpringerLink
Log in

Uptake of P from apatite by Pinus sylvestris seedlings colonised by different ectomycorrhizal fungi

  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

The role of ectomycorrhizal (EM) fungi in increasing apatite dissolution was tested in a pot system with Pinus sylvestris (L.) seedlings growing in a sand/peat mixture. Non-mycorrhizal seedlings and seedlings inoculated with one of three different isolates of EM fungi were grown for 210 days in pots divided into a root-containing and a root-free compartment. The EM mycelium was allowed to colonise the root-free compartment, to which apatite had been added as a P source in half of the pots. All mineral nutrients except P were supplied in the form of a balanced nutrient solution. Seedlings grown with apatite as their P source grew significantly better and had higher P concentrations (1.1–1.5 mg/g) compared with seedlings growing without any P source (0.6–1.0 mg/g), indicating that they were able to use apatite-P. A weathering budget based on P uptake indicated that 6.7–18.9 mg apatite was weathered per pot which corresponds to 0.3–0.9% of the added apatite. A similar budget based on Sr uptake indicated that the apatite weathering rate was lower (0.13-0.3%). One Suillus variegatus isolate and an unidentified fungus had a significant positive influence on the dissolution of apatite, while another S. variegatus isolate had colonised roots poorly and did not influence weathering significantly. Oxalic acid was detected in root-free soil and was especially abundant in compartments colonised by S. variegatus. The concentration of oxalic acid was correlated to the concentration of phosphate in the soil solution of root-free soil, indicating that oxalic acid influences apatite dissolution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen E B Allen M F Helm D J Trappe J M Molina R and Rincon E 1995 Patterns and regulation of mycorrhizal plant and fungal diversity. Plant Soil 170, 47–62.

    Article  CAS  Google Scholar 

  • Andersson S and Söderström B 1995 Effects of lime (CaCO3) on ectomycorrhizal colonization of Picea abies (L.) Karst. seedlings planted in a spruce forest. Scand. J. For. Res. 10, 149–154.

    Google Scholar 

  • Bolan N S 1991 A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant Soil 134, 189–207.

    Article  CAS  Google Scholar 

  • Cromack K Sollins P Graustein W C Speidel K Todd A W Spycher G Li C Y and Todd R L 1979 Calcium oxalate accumulation and soil weathering in mats of hypogeous fungus, Hysterangium crassum. Soil Biol. Biochem. 11, 463–468.

    Article  CAS  Google Scholar 

  • Cumming J R and Weinstein L H 1990 Utilization of AlPO4 as a phosphorus source by ectomycorrhizal Pinus rigida seedlings. New Phytol. 116, 99–106.

    Article  CAS  Google Scholar 

  • Dahlberg A and Stenström E 1991 Dynamic changes in nursery and indigenous mycorrhiza of Pinus sylvestris seedlings planted out in forest and clearcuts. Plant Soil 136, 73–86.

    Article  Google Scholar 

  • Duddridge J A 1986 The development and ultrastructure of ectomycorrhizas. III. Compatible and incompatible interactions between Suillus grevillei (Klotsch) Sing. and 11 species of ectomycorrhizal hosts in vitro in the absence of exogenous carbohydrate. New Phytol. 103, 457–464.

    Article  CAS  Google Scholar 

  • Ekblad A Wallander H Carlsson R and Huss-Danell K 1995 Fungal biomass in roots and extramatrical mycelium in relation to macronutrients and plant biomass of ectomycorrhizal Pinus sylvestris and Alnus incana. New Phytol. 131, 443–451.

    Article  Google Scholar 

  • Entry J A Rose CL and Cromack K 1992 Microbial biomass and nutrient concentrations in hyphal mats of the ectomycorrhizal fungus Hysterangium setchellii in a coniferous forest soil. Soil Biol. Biochem. 24, 447–453.

    Article  Google Scholar 

  • Entry J A, Rygiewicz P T and Emmingham W H 1994 90Sr uptake byPinus ponderosa and Pinus radiata seedlings inoculated with ectomycorrhizal fungi. Environ. Pollut. 86, 201–206.

    Article  PubMed  CAS  Google Scholar 

  • Finlay R D 1989 Functional aspects of phosphorus uptake and carbon translocation in incompatible ectomycorrhizal associations between Pinus sylvestris and Boletinus cavipes. New Phytol. 112, 185–192.

    Article  CAS  Google Scholar 

  • Fox T R, Comerford N B and McFee W W 1990. Kinetics of phosphorus release from spodosols: effects of oxalate and formate. Soil Sci. Soc. Am. J. 54, 1441–1447.

    Article  CAS  Google Scholar 

  • Gianinazzi-Pearson V and Gianinazzi S 1986 The physiology of improved phosphate nutrition in mycorrhizal plants. In Physiological and Genetical Aspects of Mycorrhizae. Eds V. Gianinazzi-Pearson & S. Gianinazzi. Proc. 1st European Symposium on Mycorrhizae, Dijon, 1-5 July 1985. pp 101–109. INRA, Paris.

    Google Scholar 

  • Griffiths R P Baham J E and Caldwell B A 1994 Soil solution chemistry of ectomycorrhizal mats in forest soil. Soil Biol. Biochem. 26, 331–337.

    Article  CAS  Google Scholar 

  • Graustein W C Cromack K Sollins P 1977 Calcium oxalate: occurrence in soils and effect on nutrient and geochemical cycles. Science 198, 1252–1254.

    CAS  PubMed  Google Scholar 

  • Harley J L 1989 The significance of mycorrhiza. Mycol. Res. 92, 129–139.

    Article  Google Scholar 

  • Harley J L and Smith S E 1983 Mycorrhizal Symbiosis. Academic Press, London.

    Google Scholar 

  • Harrison A F Stevens P A Dighton J Quarmby C Dickinson A L Jones H E and Howard D M 1995 The critical load of N for Sitka spruce forests on stagnopodzols in Wales: role of nutrient limitation. For. Ecol. Manage. 76, 139–148.

    Article  Google Scholar 

  • Ingestad T and Kähr M 1985 Nutrition and growth of coniferous seedlings at varied relative nitrogen addition rate. Physiol. Plant. 65, 109–116.

    Article  Google Scholar 

  • Kårén O and Nylund J-E (1997) Effects of ammonium sulphate on the community structure and biomass of ectomycorrhizal fungi in a Norway spruce stand in SouthWest Sweden. Can. J. Bot. 75, 1628–1643.

    Google Scholar 

  • Lapeyrie F 1988 Oxalate synthesis from bicarbonate by the mycorrhizal fungus Paxillus involutus. Plant Soil 110, 3–8.

    Article  CAS  Google Scholar 

  • Lapeyrie F Ranger J and Vairelles D 1990 Phosphate-solubilizing activity of ectomycorrhzial fungi in vitro. Can. J. Bot. 69, 342–346.

    Google Scholar 

  • Leyval C and Berthelin J 1991 Weathering of a mica by roots and rhizospheric microorganisms of pine. Soil Sci. Soc. Am. J. 55, 1009–1016.

    Article  CAS  Google Scholar 

  • Lundström U S 1994 Significance of organic acids for weathering and the podzolisation process. Environ. Int. 20, 21–30.

    Article  Google Scholar 

  • Marx D H 1969 The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots of pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi. Phytopathology 59, 153–163.

    Google Scholar 

  • Malajczuk N and Cromack K 1982 Accumulation of calcium oxalate in the mantle of ectomycorrhizal roots of Pinus radiata and Eucalyptus marginata. New Phytol. 92, 527–531.

    Article  CAS  Google Scholar 

  • Nylund J-E and Wallander H 1989 Effects of ectomycorrhiza on host growth and carbon balance in a semi-hydroponic cultivation system. New Phytol. 112, 389–398.

    Article  Google Scholar 

  • Olsson P A and Wallander H 1998 Interactions between ectomycorrhizal fungi and the bacterial community in soils with applications of different primary minerals. FEMS Microbiol. Ecol. 27, 195–205

    Article  CAS  Google Scholar 

  • Read D J, Francis R and Finlay R D 1985 Mycorrhizal mycelia and nutrient cycling in plant communities. In Ecological Interactions in Soil: Plants, Microbes and Animals. Ed. A.H. Fitter. pp 193–217. British Ecological Society Special Publication No. 4.

  • Sen R 1990 Isozymic identification of individual ectomycorrhizas synthesized between Scots pine (Pinus sylvestris L.) and isolates of two species of Suillus. New Phytol 114, 617–626.

    Article  CAS  Google Scholar 

  • Ström L Olsson T and Tyler G 1994 Difference between calcifuge and acidifuge plants in root exudation of low-molecular organic acids. Plant Soil, 167, 239–245.

    Article  Google Scholar 

  • Tinker P B 1975 Soil chemistry of phosphorus and mycorrhizal effects on plant growth. In Ectomycorrhizae. Eds F E Sanders, B Mosse and P B Tinker. pp 353–371. Academic Press, London.

    Google Scholar 

  • Unestam T and Sun Y-P 1995 Extramatrical structures of hydrophobic and hydrophilic ectomycorrhizal fungi. Mycorrhiza 5, 301–311.

    Article  Google Scholar 

  • Wallander H and Nylund J-E 1992 Effects of excess nitrogen and phosphorus starvation on the extramatrical mycelium of ectomycorrhizas of Pinus sylvestris L. New Phytol. 120, 495–503.

    Article  CAS  Google Scholar 

  • Wallander H Arnebrant K Östrand F Kårén O 1997a Uptake of 15N labelled alanine, ammonium and nitrate in Pinus sylvestris ectomycorrhiza growing in forest soil. Plant Soil 195, 329–338.

    Article  CAS  Google Scholar 

  • Wallander H Wickman T Jacks G 1997b Apatite as a P source inmycorrhizal and non-mycorrhizal Pinus sylvestris seedlings. Plant Soil 196, 123–131.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbial Ecology, Lund University, Ecology Building, 223 62, Lund, Sweden

    Håkan Wallander

Authors
  1. Håkan Wallander
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wallander, H. Uptake of P from apatite by Pinus sylvestris seedlings colonised by different ectomycorrhizal fungi. Plant and Soil 218, 249–256 (2000). https://doi.org/10.1023/A:1014936217105

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014936217105

Search

Navigation