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Advances In Biotreatment of Acid Mine Drainage and Biorecovery of Metals: 2. Membrane Bioreactor System for Sulfate Reduction

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Abstract

Several biotreatmemt techniques for sulfate conversion by the sulfate reducing bacteria (SRB) have been proposed in the past, however few of them have been practically applied to treat sulfate containing acid mine drainage (AMD). This research deals with development of an innovative polypropylene hollow fiber membrane bioreactor system for the treatment of acid mine water from the Berkeley Pit, Butte, MT, using hydrogen consuming SRB biofilms. The advantages of using the membrane bioreactor over the conventional tall liquid phase sparged gas bioreactor systems are: large microporous membrane surface to the liquid phase; formation of hydrogen sulfide outside the membrane, preventing the mixing with the pressurized hydrogen gas inside the membrane; no requirement of gas recycle compressor; membrane surface is suitable for immobilization of active SRB, resulting in the formation of biofilms, thus preventing washout problems associated with suspended culture reactors; and lower operating costs in membrane bioreactors, eliminating gas recompression and gas recycle costs. Information is provided on sulfate reduction rate studies and on biokinetic tests with suspended SRB in anaerobic digester sludge and sediment master culture reactors and with SRB biofilms in bench-scale SRB membrane bioreactors. Biokinetic parameters have been determined using biokinetic models for the master culture and membrane bioreactor systems. Data are presented on the effect of acid mine water sulfate loading at 25, 50, 75 and 100 ml/min in scale-up SRB membrane units, under varied temperatures (25, 35 and 40 °C) to determine and optimize sulfate conversions for an effective AMD biotreatment. Pilot-scale studies have generated data on the effect of flow rates of acid mine water (MGD) and varied inlet sulfate concentrations in the influents on the resultant outlet sulfate concentration in the effluents and on the number of SRB membrane modules needed for the desired sulfate conversion in those systems. The pilot-scale data indicate that the SRB membrane bioreactors systems can be applied toward field-scale biotreatment of AMD and for recovery of high purity metals and an agriculturally usable water.

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References

  • Abram JW & Nedwell DB (1978) Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch. Microbiol. 117: 89–92

    Google Scholar 

  • Badzioug W & Thauer RK (1978) Growth yields and growth rates of Desulfovibrio vulgaris (Marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as sole energy sources. Arch. Microbiol. 117: 209214

    Google Scholar 

  • Battaglia-Brunet F, Foucher S, Ignatiadis I & Morin D. (2000) Production of H2S by sulfate reducing bacteria in a two-column gas/liquid reactor for the purification of metal-containing effluents. XXI IMPC. Roma, 2327 July 2000, pp. B 129/17

  • Battaglia-Brunet F, Foucher S, Denamur A. Ignatiadis I, Michel C & Morin D (2002) Reduction of chromate by fixed films of sulfatereducing bacteria using hydrogen as an electron source. J. Indust. Microbiol. Biotechnol. 28: 154–159

    Google Scholar 

  • Brandis A & Thauer R (1981) Growth of Desulfovibrio species on hydrogen and sulfate as sole energy source. J. Gen. Microbiol. 126: 249–252

    Google Scholar 

  • Brandt KK, Vester F, Jensen AN & Ingvorsen K (2001) Sulfate reduction dynamics and enumeration of sulfate reducing bacteria in hypersaline sediments of the Great Salt Lake (Utah, USA). Microb. Ecol. 41: 1–11

    Google Scholar 

  • Brindle K & Stephenson T (1996) The application of membrane biological reactors for the treatment of wastewaters. Biotechnol. Bioeng. 44: 596–594

    Google Scholar 

  • Buisman CJN, Uspeert P, Hol A, Janssen AJH, tenHagen R & Lettinga G (1991) Kinetic parameters of a n-fixed culture oxidizing sulfide and sulfur with oxygen. Biotechnol. Bioeng. 38: 813–820

    Google Scholar 

  • Choo KH & Lee CH (1996) Membrane fouling microorganisms in the membrane-coupled anaerobic bioreactor. Wat. Res. 30: 1771–1780

    Google Scholar 

  • Cote P, Bersillon JL, Huyard A & Faup G (1988) Bubble-free aeration using membrane process analysis. J.Wat. Pollut. Contr. Fed. 60: 1986–1992

    Google Scholar 

  • Du Preez LA, Odendaal JP, Maree JP & Pansenby M (1992) Biological removal of sulfate from industrial effluents using producer gas as energy source. Environ. Technol. 13: 875–882

    Google Scholar 

  • Dvorak DH, Hedin RS, Edenborn HM & McIntire PE (1992) Treatment of metal contaminated water using bacterial sulfate reduction: Results from pilot scale reactors. Biotechnol. Bioengr. 40: 609–616

    Google Scholar 

  • Federovich V, Greben M, Kalyuzbnyi S, Lens P & Hulshoff Pol LW (2000) Use of hydrophobic membranes to supply hydrogen to sulfate reducing bioreactors. Biodegradation 11: 295–303

    Google Scholar 

  • Foucher S, Battaglia-Brunet A, Ignatiadis I & Mpron D (2001) Treatment by sulfate-reducing bacteria of Chessy acid-mine drainage and metals recovery. Chem. Bioengr. Sci. 56: 1639–1645

    Google Scholar 

  • Ghyoot WR & Verstraete WH ( 1997) Coupling membrane filtration to anaerobic primary sludge digestion. Environ. Technol. 18: 569–580

    Google Scholar 

  • Govind R, Rao P & Tabak HH (2000) Membrane reactor studies for treatment of acid mine drainage. U.S. EPA Report, National Risk Management Research Laboratory, ORD, Cincinnati, OH, 30 September 2000, pp.85

    Google Scholar 

  • Greben M (1999) Biological sulfate reduction in a membrane bioreactor. Ms. Thesis Wageningen Agricultural University, Wageningen, The Netherlands

    Google Scholar 

  • Holder EL & Tabak HH (2002) Use of respirometry to measure hydrogen consumption by sulfate reducing bacteria in the presence of toxic metals. SETAC 23rd Annual Meeting in North America, Salt Lake City, Utah, USA, 16–20 November 2002

  • Hulshoff Pol L, Lens P, Stains AJM & Lettinga G (1998) Anaerobic treatment of sulfaterich wastewaters. Biodegradation 9: 213–224

    Google Scholar 

  • Ingvorsen K & Jorgensen BB (1984) Kinetics of sulfate uptake by freshwater and marine species of Desulfovibrio. Arch. Microbiol. 139: 61–68

    Google Scholar 

  • Ingvorsen K, Zehnder AJB & Jorgensen BB (1984) Kinetics of sulfate and acetate uptake by Desulfovibrio postgatei. Appl. Environ. Microbiol. 47(1): 403–408

    Google Scholar 

  • Ito T, Okabe S, Satoh H & Watanabe Y (2002) Successional development of sulfate reducing bacterial populations and their activities in a wastewater biofilm growing under microaerophilic conditions. Appl. Environ. Microbiol. 68(3): 1392–1402

    Google Scholar 

  • Kalyuzhnyi S and Fedorowich V (1997) Integrated mathematical model of UASB reactor for competition between sulfate reduction and methanogenesis. Wat. Sci. Technol. 36(6–7): 201–208

    Google Scholar 

  • Lovley DR, Dwyer D & Klug MJ (1982) Kinetic analysis of competition between sulfate reducers and methanogenesis for hydrogen in sediments. Appl. Environ. Microbiol. 43: 1373–1379

    Google Scholar 

  • Lupton FS, Conrad R & Zeikus JG (1984) Physiological function of hydrogen metabolism during growth of sulfidogenic bacteria on organic substrates. J. Bacteriol. 159(3): 843–849

    Google Scholar 

  • Lupton FS & Zeikus JG (1984) Physiological basis for sulfatedependent hydrogen competition between sulfidogens and methanogens. Curr. Microbiol. 11: 7–12.

    Google Scholar 

  • Maree JP & Hill E (1989) Biological removal of sulfate from industrial effluents and concomitant production of sulfur. Wat. Sci. Tech 21: 265276

    Google Scholar 

  • Mizuno O, Takagi H & Noike T (1998) Biological sulfate removal in an acetogenic bioreactor with an ultrafiltration membrane system. Wat. Sci. Tech. 38(4/5): 513–520

    Google Scholar 

  • Nanninga HJ & Gottschal (1987) Properties of Desulfovibrio carbonicolicus sp. Nov. and other sulfate-reducing bacteria isolated from an anaerobic-purification plant. Appl. Environ. Microbiol. 53(3): 802–809

    Google Scholar 

  • Nielsen PH (1987) Biofilm dynamics and kinetics during highrate sulfate reduction under anaerobic conditions. Appl. Environ. Microbial. 53: 27–32

    Google Scholar 

  • Noguera DR, Brusseau GA & Rittmann B & Stahl DA (1998) A unified model describing the role of hydrogen in the growth of Desulfovibrio vulgaris under different environmental conditions. Biotechnol. Bioengr. 59(6): 732–746

    Google Scholar 

  • Odom JM & Peck Jr. HD (1981) Hydrogen cycling as a general mechanism for energy coupling in the sulfate-reducing bacteria, Desulfovibrio sp. FEMS Microbiol. Lett. 12: 47–50

    Google Scholar 

  • Okabe, S. Nielsen PH, Jones WL & Charaklis WG (1995) Rate and Stoichiometry of microbial sulfate reduction by Desulfovibrio desulfuricans in biofilms. Biofouling 9: 63–83

    Google Scholar 

  • Okabe S, Itoh, T, Satob H & Watanabe Y (1999) Analyses of spatial distribution of sulfate reducing bacteria and their activity in aerobic wastewater biofilms. Appl. Environ. Microbiol. 65: 5107–5116

    Google Scholar 

  • Pankhania M, Stephenson T & Semmens MJ (1994) Hollow fibre bioreactor for wastewater using bubbleless membrane aeration. Wat. Res. 28:10 2233–2236

    Google Scholar 

  • Paques Biosystems BV PO Box 52, 8560 AB Balk, The Netherlands (2000) Bioprocess Technology: Sulfate removal and metal recovery; Heavy metals removal; Hydrogen sulfide removal. Pamphlets and pers. comm.

  • Phelps TJ, Conrad R and Zeikus JG (1985) Sulfate-dependent interspecies hydrogen transfer between Methanosarcina barkeri and Desulfovibrio vulgaris during coculture metabolism of acetate and methanol. Appl. Environ. Microbiol. 50(3): 583–594

    Google Scholar 

  • Ramsing NB, Kuhl M & Jorgensen BB ( 1993) Distribution of sulfate reducing bacteria, 02 and H2S in photosynthetic biofilms determined by oligonucleotide probes and microelectrodes. Appl. Environ. Microbiol. 59: 3840–3849

    Google Scholar 

  • Rinzema A & Lettinga G (1988) Anaerobic treatment of sulfate containing wastewater. In: Wise DL (Ed) Biotreatment Systems 3, CRC Press, Boca Raton, FL pp. 65–109

    Google Scholar 

  • Robinson JA & Tiedje JM (1984) Competition between sulfatereducing and methanogenic bacteria for hydrogen under resting and growing conditions. Arch. Microbiol. 137: 26–32

    Google Scholar 

  • Ruthemond C, Camper A & Wilderer PA (1994) Biofilms growing on gas permeable membranes. Wat. Sci. Tech. 29: 11447–11454

    Google Scholar 

  • Santegoeds CM, Ferdelman TG, Muyzer G & de Beer D (1998) Structural and functional dynamics of sulfate reducing populations in bacterial biofilms. Appl. Environ. Microbiol. 64: 3731–3739

    Google Scholar 

  • Shimada K (1987) Removal of heavy metals from mine wastewater using sulfate reducing bacteria. J.Wat.Wastewat. Jpn. 31: 52–56

    Google Scholar 

  • Sipma J, Lens P, Vieira A, Miron Y, Van Lier JB, Hulshoff Pol LW & Lettinga G (1999) Thermophilic sulphate reduction in upflow anaerobic sludge bed reactors under acidifying conditions. Proc. Biochem. 35(5): 509–522

    Google Scholar 

  • Sorensen J, Christensen D & Jorgensen BB (1981) Volatile fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl. Environ. Microbiol. 42(1): 5–11

    Google Scholar 

  • Traore AS, Fardeau M-L, Hatchikian CE, LeGall J & Belaich JP (1983) Energetics of growth of a defined mixed culture of Desulfovibrio vulgaris and Methanosarcina barkeri: Intraspecies hydrogen transfer in batch and continuous cultures. Appl. Environ. Microbiol. 46(5): 1152–1156

    Google Scholar 

  • Van Houten RT, Van der Spoul H, Van Aelst AC, Hulshoff Pol, LW & Lettinga G (1996) Biological sulphate reduction using synthesis gas. Biotechnol. Bioengr. 50: 136–144

    Google Scholar 

  • Vick RTB, Blanch HW & Wilke CR (1983) Microbial hollow fiber bioreactors. Trends in Biotechnol. 1: 135–139

    Google Scholar 

  • Weijma J, Hulshoff Pol LW, Stams AJM & Lettinga G (2000) Performance of a thermophilic sulfate and sulfite reducing high rate anaerobic reactor fed with methanol. Biodegradation 11(6): 429–439

    Google Scholar 

  • Widdel F & Pfennig N (1982) Studies on dissimilatory sulfatereducing bacteria that decompose fatty acids. II. Incomplete oxidation of propionate by Desulfovibrio propionicus gen. nov., sp, nov. Arch. Microbiol. 131: 360–365.

    Google Scholar 

  • Widdel F & Bak F (1991) Gram-negative mesophilic sulfatereducing bacteria. In: Balows A, Truper HG, Dworkin M, Harder W & Schleifer KH (Eds). The Prokaryotes. 2nd edn. Springer-Verlag, New York. pp. 3352–3378.

    Google Scholar 

  • Widdel F & Hansen TA (1991) The dissimilatory sulfate and sulfur-reducing bacteria. In Balows A, Truper HG, Dworkin M, Harderand W & Scleider K-H (Eds) The Prokaryotes, 2nd edn. (pp. 583–624). Springer-Verlag, New York.

    Google Scholar 

  • Yamamoto K, Hiasa M, Mahmood T & Matsuo T (1989) Direct solid-liquid separation using hollow fiber membrane in an activated sludge aeration tank. Wat. Sci. Technol. 21: 43–49.

    Google Scholar 

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Authors and Affiliations

  1. US Environmental Protection Agency, ORD, NRMRL, Cincinnati, OH, 45268, USA

    Henry H. Tabak

  2. Department of Chemical Engineering, University of Cincinnati, Cincinnati, OH, 45221, USA

    Rakesh Govind

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  1. Henry H. Tabak
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  2. Rakesh Govind
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Tabak, H.H., Govind, R. Advances In Biotreatment of Acid Mine Drainage and Biorecovery of Metals: 2. Membrane Bioreactor System for Sulfate Reduction. Biodegradation 14, 437–452 (2003). https://doi.org/10.1023/A:1027332918844

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