Abstract
The 5′-upstream non-coding region of an FI-carboxymethyl cellulase (CMCase) gene of Aspergillus aculeatus No. F-50 was obtained and sequenced up to −777 nucleotide in pCMG23. The 5′-upstream region of different lengths were constructed and fused to a reporter gene (Escherichia coli lacZ) in pAN923-42BD, and the resulted constructs were introduced into the A. nidulans. The β-galactosidase activities of the transformant with 5′-upstream fragment of larger than 319 bp were expressed by the induction, but that of 109 bp drastically decreased to the basal level. This suggests that the region between −109 and −319 of the 5′-upstream non-coding region is involved in the regulation of the FI-CMCase expression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bahl CP, Wu R, Narang SA (1980) Meth. Enzymol. 65: 877–885.
Bradford JF (1976) Anal. Biochem. 82: 267–270.
Cubero B, Scazzocchio C (1994) EMBO J. 13: 407–415.
Dowzer CEA, Kelly JM (1991) Mol. Cell. Biol. 11: 5701–5709.
Fantes PA, Roberts CF (1973) J. Gen. Microbiol. 77: 471–486.
Hanahan D (1983) J. Mol. Biol. 166: 557–580.
Hata Y, Natori K, Katsube Y, Ooi T, Arai M, Okada H (1994) J. Mol. Biol. 214: 278–280.
Kulmburg P, Mathieu M, Dowzer C, Kelly J, Felenbok B (1993) Mol. Microbiol. 7: 847–857.
Mathieu M, Felenbok B (1994) EMBO J. 13: 4022–4027.
Murao S, Sakamoto R, Arai M (1988) Meth. Enzymol. 160: 274–299.
Ooi T, Shinmyo A, Okada H, Hara S, Ikenaka T, Murao S, Arai M (1990a) Curr. Genet. 18: 217–222.
Ooi T, Shinmyo A, Okada H, Murao S, Kawaguchi T, Arai M (1990b) Nucleic Acids Res. 18: 5884.
Pinaga F, Fernandez-Espinar MT, Valles S, Ramon D (1994) FEMS Microbiol. Lett. 115: 319–324.
Sanger F, Nicklen S, Coulson AR (1977) Proc. Natl. Acad. Sci. USA 74: 5463–5467.
Stangl H, Gruger F, Kubicek CP (1993) Curr. Genet. 23: 115–122.
Strauss J, Mach RJ, Zeilinger S, Hartler G, Stoffler G, Wolschek M, Kubicek CP (1995) FEBS Lett. 376: 103–107.
Takashima S, Iikura H, Nakamura A, Masaki H, Uozumi T (1996) FEMS Microbiol. Lett. 145: 361–366.
Takashima S, Nakamura A, Hidaka M, Masaki H, Uozumi T (1998) Biosci. Biotechnol. Biochem. 62: 2364–2370.
Tilburn J, Sarkar S, Widdick DA, Espeso EA, Orejas M, Mungroo J, Panelva MA, Arst HN Jr (1995) EMBO J. 14: 779–790.
Van Gorcom RFM, Punt PJ, Pouwels PH, van den Hondel CAMJJ (1986) Gene 48: 211–217.
Yanish-Perron C, Vieira J, Messing J (1985) Gene 33: 103–109.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ooi, T., Morotomi, K., Kinoshita, S. et al. Analysis of 5′-upstream non-coding region of FI-carboxymethyl cellulase gene from Aspergillus aculeatus . Biotechnology Letters 21, 735–739 (1999). https://doi.org/10.1023/A:1005534925774
Issue Date:
DOI: https://doi.org/10.1023/A:1005534925774