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Optical collection of extracellular vesicles in a culture medium enhanced by interactions with gold nanoparticles

  • Special Issue: Original Paper
  • Novel Analytical Approaches towards SDGs
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Abstract

Extracellular vesicles (EVs) exist in biological fluids such as blood, urine, and cerebrospinal fluid and are promising cancer biomarkers. Attempts to isolate and analyze trace EVs, however, have been a challenge for researchers studying their functions and secretion mechanisms, which has stymied the options for diagnostic application. This study demonstrated a collection of EVs that was enhanced by gold nanoparticles (AuNPs) via the use of optical force. The collection system consists of an inverted microscope equipped with a CCD camera, a square capillary connected with a PTFE tube, and an Nd:YAG laser that generates optical force. The laser beam was focused on a capillary wall in which a cell culture medium containing EVs flowed continuously. Control of the surface charges on both the capillary wall and the AuNPs achieved the collection and retention of EVs on the capillary wall. The positively charged capillary wall retained EVs even after the laser irradiation was halted due to the negative charges inherent on the surface of EVs. Conversely, positively charged AuNPs had a strong electrostatic interaction with EVs and enhanced the optical force acting on them, which made collecting them a much more efficient process.

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Data availability statement

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Numbers JP19H04675 and JP20H02766.

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Authors and Affiliations

  1. Department of Chemistry, Okayama University, Okayama, 700-8530, Japan

    Yumeki Tani, Kenta Ochiai & Takashi Kaneta

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  1. Yumeki Tani
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  2. Kenta Ochiai
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  3. Takashi Kaneta
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Correspondence to Takashi Kaneta.

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The authors declare that they have no known competing financial interests or personal relationships that could appear to influence the work reported in this paper.

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Tani, Y., Ochiai, K. & Kaneta, T. Optical collection of extracellular vesicles in a culture medium enhanced by interactions with gold nanoparticles. ANAL. SCI. 39, 643–651 (2023). https://doi.org/10.1007/s44211-022-00207-2

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  • DOI: https://doi.org/10.1007/s44211-022-00207-2

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