Fishermen’s perceptions and experiences toward the impact of climate change and anthropogenic activities on freshwater fish biodiversity in Côte d’Ivoire

Africa’s freshwater ecosystems are impacted by many threats due to climate change and several anthropogenic activities. In Côte d’Ivoire, the economy relies mostly on agriculture, which covers around 64% of the land surface. The study aimed to investigate Côte d’Ivoire fishermen’s perception of climate change, its drivers and other anthropogenic activities impacting freshwater fish biodiversity. The fish species locally threatened in the Ivorian freshwater ecosystems were also investigated. Face to face interviews and a structured questionnaire were used. The data obtained were analysed using descriptive statistics such as frequency, percentage, means, and standard deviation, minimum and maximum. The multinomial logit regression was also applied to test factors that influence fishermen’s awareness and perception of climate change and their perception of change in fish abundance. The results show that around 72.7% of fishermen were aware of climate change. Decreases of precipitation (87.9%) and increases of temperature (76.1%) were observed by fishermen. Also, a significant decrease in fish quantity was observed by respondents. Climate change and anthropogenic activities such as gold mining, water withdrawal, use of small-mesh fishing nets, overfishing, industrial waste pollution, pesticides use for agricultural purposes along watersheds, obnoxious fishing practices and population growth, were highlighted as the main reasons for this decline. Species such as Lates niloticus, Heterotis niloticus, Parachanna obscura, Malapterurus electricus, Sarotherodon galilaeus, Tilapia zillii, Polpyterus endlicheri and Labeo coubie were listed by fishermen as locally threatened within Ivorian freshwater ecosystems. Actions and policies are needed to be taken by local authorities to ensure the sustainability of fisheries resources. The information obtained from this study constitutes a prerequisite for developing and implementing studies or policies aimed at mitigating and combating the negative effect of climate change and anthropogenic activities on the Ivorian freshwater ecosystem.


Sampling of fishermen
The study was conducted as part of a project titled "Predicting the impact of climate change and anthropogenic activities on the distribution and genetic diversity of Parachanna species in Côte d'Ivoire" funded by the West African service centre of Climate Change and Adapted Land-use scholarship programme, for three years and half (started in September 2019). Thus, a preliminary investigation was done in the three selected climatic zones with 90 fishermen, to identify the proportion of them fishing or trading on Parachanna fish species among their global target species. The fishermen selected were those who were familiar with Parachanna species and who had already been involved in fishing or trading on Parachanna species. These fishermen were also involved in fishing or trading other common fish species found at the sampling sites. The survey was conducted among fishermen in different types of freshwater ecosystems, including lakes, rivers and streams. The investigation resulted in the proportion of 60% of fishermen fishing on Parachanna among the global interviewed fishermen. This proportion was used in [23] formula with a 95% confidence level to calculate the necessary sample size: N = the total number of fishermen to be surveyed; U 2 1 −α /2 represents the value of the normal random variable for a probability value of 1-α/2 = 0.975; U 2 1 −α /2 = 1.96; p represents the estimated proportion of fishermen fishing on Parachanna fish species among their target species, obtained from the preliminary investigation (p = 0.60); d is the margin of error of a parameter estimated from the sample fixed at 0.05. From the formula, the sample size of 369 fishermen was the minimum needed, but to be more accurate, 381 fishermen were interviewed in this study.

Data collection
Face to face interviews were conducted using a structured questionnaire from October 2020 to February 2021. Fishermen were randomly selected on each sampling site. Fish landing sites within each sampling site were used as collection sites. The questionnaire was divided into three sections (Appendix 1). The first section was structured to assess the respondent's general demographic background (e.g., age, sex, household size, education level, occupation, monthly income, etc.) and the second section was on the fishermen's knowledge of climate change (e.g., awareness, causes and drivers, perceived change in variables, etc.). The last section was linked to respondents' perception of the impact of climate change and human activities on freshwater fish biodiversity (e.g., change in fish quantity, how climate change drew change in fish biodiversity, major human activities threatening freshwater fish biodiversity, locally threatened fish species).

Data analysis
Descriptive statistics (frequency, percentage, means and standard deviation, minimum and maximum) were calculated to analyse fishermen's demographic information, their perception related to climate change and its impact on freshwater fish biodiversity and their perception of human impact on fish biodiversity. The multinomial logit regression model (MLN) was applied to analyse factors that influence fishermen's awareness of climate change, their perception of climate change, and their perception of change in fish abundance. This model has been widely used in many studies to test factors that influence individual knowledge of climate change, climate adaptation and climate change risk perception [24,25]. The multinomial logit regression is used to predict the probability of category membership of a dependent variable based on several independent (predictor) variables. The MLN model is a simple extension of the binary logit regression that allows for more than two categories of the dependent or independent variables. In this model, the independent variables can either be nominal or ordinal [26]. MLN uses maximum likelihood estimation to evaluate the probability of categorical membership in a dependent variable based on multiple predictor variables. In this study, we considered sociodemographic variables (e.g., age, gender, education level, household size, ethnic group) of fishermen as the predictors and perception variables (e.g., awareness of climate change, perceived change in climate or fish quantity) as dependent variables. The MLN results reflect the expected change in the probability that fishermen perceive climate change or fish quantity change or be aware of climate change with respect to a one-unit change in a predictor variable, keeping all the other predictors constant. Alpha (α) lesser than 0.05 was considered statistically significant. SPSS version 2.2 software was used for the descriptive statistics, while the multinomial logit regression was implemented in R 3.6.3 software.

Livelihood strategies of the fishermen
Whereas 80% of the respondents were engaged in fishing as their main occupation, 14.2% were only engaged in fish trade. The remaining percentage was shared among farming, teaching and shopkeeping. Regarding the secondary occupation, respondents were mostly involved in farming (59.1%), followed by fishing (3.9%), fish trading (2.6%), livestock farming (1.6%), and others (11.8%). Around 21% of respondents were not engaged in any secondary activities. Concerning fishermen'' monthly incomes, the arithmetic mean monthly income was about 130 EUR, with a minimum of 15 EUR and a maximum of 763 EUR. However, it varied among the climate zones. Thus, Guinean climate zone fishermen had the highest mean monthly incomes of about 145 EUR. Comparatively, Sudano-Guinean climate zone fishermen recorded the middle monthly mean income (126 EUR), more than those of Sudanian climate zone with the lowest one (93 EURO). The complete results of the interviewees' livelihood are presented in Tables 2 and 3.

Fishermen's perception of climate change, its drivers, its impact on Ivorian freshwater fish abundance
About 72.7% of fishermen were aware of climate change, whereas 27.3% were not. Of the total percentage of the respondents who were aware of climate change, 53% and 24% believed that the drivers of climate change are deforestation and greenhouse gas emissions, respectively, whereas 20% of the respondents attribute the cause of climate change to the will of God, and 2% believe that natural processes such as volcanic eruptions are the main cause of climate change (Fig. 2). A decrease in precipitation (87.9%) and an increase in temperature (76.1%) were observed by respondents. Additionally, interviewees testified to have observed delay in the onset of the rainy season and its length shortening (Fig. 3). Concerning the occurrence of floods, 55.6, 3.7, and 40.7% of the respondents observed an increase, decrease and no change respectively ( Table 4).
Most of the fishermen reported significant decreases in fish abundance (81.6%), while 10.0, 3.4, and 5% observed little, moderate and no decrease, respectively. Concerning the impact of climate change on fish abundance, the respondents stated that change in temperature has mainly led to a decrease in fish abundance (65.9%), while 3.4% of the respondents argued that change in temperature has led to an increase in fish abundance, 22.8% observed no effect and 7.9% did not know. As far as the effect of change in precipitation on the fish abundance is concerned, 71.1% of the respondents specified that it has led to a decrease, 8.1% to an increase, and 5% did not know. For the remaining 15.7%, change in precipitation has not affected fish abundance.  Table 4 shows the perception of interviewees on the impact of human activities on the Ivorian freshwater fish. Gold mining, water withdrawal for human needs, use of small-mesh fishing nets, overfishing, industrial waste discharge, pesticides use for agricultural purposes along watersheds, obnoxious fishing practices and increase in human population, were identified by Ivorian fishermen as the major anthropogenic perturbations affecting Côte d'Ivoire freshwater fish biodiversity. Specifically, fishermen of Bia River argued that the river is mainly impacted by obnoxious fishing practices (44.8%), overfishing (31.0%) and gold mining (22.4%). The use of small-mesh fishing nets (1.7%) was also identified as a stressor for the fish biodiversity of this river. Fishermen in the artificial lake Ayame, resulting from the construction of the hydroelectric dam on Bia River, identified in decreased order, gold mining (44.8%), overfishing (41.4%), water withdrawal and obnoxious fishing practices as stressors of the fish biodiversity in this lake. Use of small-mesh fishing nets, water withdrawal and industrial waste discharge were recognised as factors threatening fish diversity of lake Faye. Pesticides used for agricultural purposes along watersheds were implicated as the main human activity that threatens Buyo lake and Nzo River fish communities. Regarding Kan Lake located within the Sudano-Guinean climate zone and Bagoue river located in the Sudanian climate zone, overfishing was perceived to be the principal factor of fish biodiversity decline.

Fish species locally under threats according to the fishermen
This section summarizes the fish species within Ivorian freshwaters for which fishermen perceived drastic population declines, such that they are considered to be very scarce over the past years. Tilapia zillii and Malapterurus electricus were the principal fish species listed by Bia River fishermen, while those of Ayame lake mainly identified Heterotis niloticus and Parachanna obscura. Labeo cubii, Parachanna obscura and Tilapia zillii species were mostly reported threatened within buyo Lake. Meanwhile, Heterotis niloticus was the only species reported threatened by Faye Lake fishermen. About 89.1% of the interviewees from the area surrounding Kan Lake identified Tilapia zillii as under threat in this lake. About River Bagoue and lake Nzo, no species was identified by the fishermen as being threatened in these water bodies (Table 5).

Factors influencing fishermen's awareness of climate change and perception of change climate
Tables 6 and 7 present the results of the multinomial logit regression models concerning the variables that influence respondents' awareness and perception of climate change.
Variables such as climatic zone, household size and education level were significantly associated with respondents' awareness of climate change. Positive relationships between respondents' education level and awareness of climate change were found, meaning that respondents' awareness increased with high education level. The same positive relationship was found with respondents' household size, which increased fishermen's awareness of climate change. However, a negative relationship was obtained between fishermen's awareness of climate change and the climate zone. Thus, respondent awareness of climate change decreased when going from the Guinean forest zone to the Sudanean savanna zone.
Concerning fishermen's perception of climate change, age, household size, gender, and education level were strongly influencing fishermen's perception of change in rainfall. A decrease in rainfall was mainly observed by young adults, males, and low education level fishermen. Perceived change in temperature was significantly influenced by fishermen's fishing watersheds and ethnic groups. Perception of temperature decreases decreased when going from Bia River, Lake Ayame, Lake Buyo, Lake Faye, Lake Kan, Bagoue River to Nzo River fishermen. Perceived change in flooding was significantly determined by watersheds, ethnic group, main occupation and household size.

Factors determining fishermen's perception of change in fish quantity
Change in fish abundance was significantly associated with respondents' age, education level, ethnic group and climate zone. Perceived decrease in fish abundance (low, moderate, and significant) increases with an increase in respondents' ages. Positive relationships between fishermen's education level and their perception of significant decrease in fish abundance were observed (Table 8). Fishermen's perception of significant decrease in fish abundance increased when going from the Guinean climate zone to the Sudanean zone.

Discussion
Climate change has become the focus of scientists, politicians, policymakers and the general public since the last decade. The representatives of indigenous peoples have been pushing to engage with climate change agreements since the adoption of the Kyoto Protocol in 1997 [27]. This study, which aims to capture Ivorian local fishermen's perceptions and personal experience of climate change, represents an important contribution to the global effort of combating and reducing climate change. Fishermen are directly dependent on the aquatic ecosystems for their livelihoods. Thus, understanding their perception and knowledge of climate change is an essential step towards developing and implementing adaptation and mitigation strategies to deal with the effects of climate change on aquatic ecosystems.
The high proportion of awareness of climate change obtained is consistent with a study by [28] who argued that the majority of Africa's citizens are aware of change in climate patterns. Many other studies conducted in several parts of Africa found similar results with households [29], farmers [24] and fishers [30,31]. Since the twentieth century, increases in temperature and decreases in precipitation have been experienced in Cote d'Ivoire [20,32]. In our study, fishermen mainly observed an increase in temperature, a decrease in precipitation and an increase in flood occurrence. Ivorian  fishermen are therefore well informed of the climate trend. Being aware of local perception and knowledge of climate change is essential for formulating climate change education, communication interventions, and for effectively implementing risk reduction strategies [25]. Our result, which suggests that most of the Ivorian fishermen are well informed of climate change, is a positive feature for global actions against climate change in Cote d'Ivoire. Local fishermen should be associated with the process of decision-making concerning climate change adaptation and mitigation strategies.
According to previous studies, the demographic background of individuals represents an important factor that influences their awareness and perception of climate change [24,29,33,25]. For instance, [25] have shown that sociodemographic factors such as gender, age and education have a great influence on farmers' knowledge and perception of climate change. The result of the multinomial logit regression model, which highlighted the significant importance of demographic variables such as age, gender, household size ethnic group and education level in fishermen awareness and perception of climate change, corroborates these findings. Interviewees' awareness of "climate change" was positively influenced by educational level. Our finding is consistent with those of [29] and [34] which showed an increase of respondents' knowledge of climate with education level. Thus, people with higher educational levels are more likely to get access to information related to climate change than those with low educational levels. The same observations were  done by [35], where higher levels of knowledge of individuals allowed them to better assess the threats posed by climate change and reduced the perceived level of uncertainty related to climate change and the impact of pro-environmental behaviours.
Freshwater fishes represent an important and vital component of the food of many people in sub-Saharan Africa [11]. Our study suggests that most of the respondents observed significant declines in fish quantity. Climate change, especially change in temperature and rainfall were acknowledged to contribute to this decline in Ivorian freshwater fish abundance. Additionally, many anthropogenic activities including gold mining, water withdrawal for human needs, use of small-mesh fishing nets, overfishing, industrial waste discharge, pesticides use for agricultural purposes along watersheds, obnoxious fishing practices and increase in human population, were listed by fishermen as other stressors contributing in depletion of Ivorian freshwater fish abundance. Since the independence of Côte d'Ivoire, a set of laws and regulations has been adopted for the sustainable management of the fisheries sector [36]. The most recent is the law n° 2016-554 of 26 July 2016 on fisheries and aquaculture, which provides dispositions relating to the regulation and sustainability of aquatic resources, in particular in its articles 8, 9, 10, 11 and 12 [37]. The dispositions in these articles define the set of measures such as the types of fishing engines allowed, the size of fishing nets allowed, the prohibition of fishing on protected species, the prohibition of the use of any explosive materials, toxic substances or baits likely to weaken, stun, excite or kill aquatic resources or to infect their habitats, etc. However, based on information provided by fishermen, there is clear evidence of a lack of application of these measures by fishermen communities. Thus, awareness-raising campaigns on the important values of freshwater ecosystem biodiversity, especially those of fish species, for human livelihoods, and the necessity to preserve these ecosystems for the benefit of future generations are necessary. Similarly, populations living in areas surrounding freshwater habitats should be informed on practices that negatively impact the biodiversity of freshwater ecosystems. In addition, further measures such as a prohibition on fishing during reproduction seasons of fish species, introduction of laws imposing prison sentences to those engaged in obnoxious fishing practices, establishment of buffer zones between watersheds and agriculture farms in order to prevent pesticides used in agriculture to get water bodies, enforcement of mining and agricultural industries to treat their waste before discharging it into waterways, should be taken by the Ivorian government in agreement with local fishery management authorities. These additional measures will contribute to strengthening the existing aquatic resources management policy, then allow the sustainable use of these resources. To reduce the effect of climate change on fish abundance, greenhouse gas emissions and deforestation must be reduced, and reforestation campaigns should be increased all over the country. Another solution for the mitigation of climate change is the protection of watershed forests.
The listed factors threatening Ivorian freshwater fish biodiversity in our study are among the wide spectra of factors threatening Africans' freshwater ecosystems in general. Indeed, according to [11], climate change, water pollution, habitat loss due to deforestation, overfishing, mining, and agriculture represent the greatest threats to freshwater fishes in Africa and specifically in West Africa [11]. For instance, impact of climate change was observed in Lake Chad located in West Africa between 1960s and 2001, where the surface area of water was reduced to 5.4% of its initial level (50% caused by climate change), leading to the loss of many aquatic organisms taxa [9,38]. Also, according to [39], natural disasters (e.g., droughts) represent the second most serious cause of decline for around 70% of the freshwater fishes in northern Africa. In Kinshasa and Lagos, pollution from oil exploration, cars in the cities, factories or other urban industries, and from boat traffic are impacting freshwater ecosystems. Pollution from oil exploration, combined with habitat loss, specifically threatens many restricted-range species in the Niger Delta and may pose a threat to species in coastal freshwater of the Republic of Congo, Cabinda, Gabon and Angola [9]. Congo freshwater ecosystem was additionally reported to suffer from mercury discharge linked to gold mining activities. In Lower Guinea, freshwater species are particularly threatened by intensive land use and pollution from rubber, banana, and palm oil plantations [9]. Another example is the case of eastern slopes of Mount Cameroon, where freshwater fish biodiversity is impacted by habitat loss due to agriculture. In Benin and the Volta region of Africa, overfishing was reported to cause changes in fish community structures and distributions [40,11].
Research studies examining the survival of extremely rare, cryptic or possibly extinct species often rely on local knowledge rather than on direct study of these species. While there is considerable potential for error and bias in the collection and interpretation of traditional ecological knowledge from local informants [41], this research approach provides a unique source of data with substantial applicability for conservation [42,43]. Fish species populations declines have been supported by fisher's perceptions in several studies that have assessed freshwater fish species [42] and the marine one as well [44][45][46]. The study by [44] in the Eastern Brazilian Marine Park corroborated the decline of seven of the reef fish species by fishers. These declines were mostly due to heavy fishing pressure and overfishing along the Brazilian coast [44]. Another example is the study by [42], which demonstrated the strong awareness of fishers of the major declines in many fish species in the Yangtze River in recent decades. In our study, the species reported by respondents as locally scarce or under threat or with their populations in depletion are all classified in the category of least concern in the IUCN red list. Heterotis niloticus is a pelagic species that frequent shallow waters of rivers and lakes for the adults, and marshy places among aquatic vegetation for the young [47]. Tilapia zillii, Parachanna obscura and Lates niloticus are demersal and potamodromous species. Tilapia zillii is caught in shallow marginal waters, drowned forest areas and also on sandy shores [48]. Lates niloticus inhabits channels, lakes and irrigation canals, prefers sandy bottoms, but is also found in rocky to muddy bottoms. Vegetated areas in calm waters provide shelter for its larvae and young [49]. Parachanna obscura is a deep-water inhabitant and migratory freshwater species found mainly in floodplains, swamps, rivers, lakes, ponds and streams [50]. This species breeds throughout the year, but preferably during and just after floods. Sarotherodon galilaeus is also demersal and occurs in the shallow coastal waters of lakes and prefers open water, but juveniles and breeding adults can be found offshore [51]. In lakes, it is often associated with beds of submerged vegetation. Malapterurus electricus inhabits shallow waters with muddy or sandy bottoms, close to rocky areas, and prefers stagnant or slow-moving water [52]. Labeo coubie is a benthopelagic and potamodromous species living in rivers and lakes, and mainly sheltered bays [53]. All of these species breed on seasonal floodplains, overflooded areas or in marginal vegetated areas of the catchment. Thus, any change in the rainy season pattern (late or early arrival, shortening, etc.) or any reduction in rainfall can disrupt their reproduction and spawning. Also, given that fish are often adapted to a certain level of hydrological variability, changes in this variability could have negative effects on fish populations [54]. Unusually large floods or seasonal pulses can displace adults and displace or injure juveniles and larvae, and droughts or prolonged dry spells can lead to population declines as well as reductions in abundance and changes in species composition [55,54]. According to the fishermen, there has been a decrease in rainfall and a change in the onset and length of the rainy season as well as an increase in floods occurrence. Thus, this can probably explain why these species were reported as locally threatened by respondents. Freshwater fish are all exotherms, unable to regulate their body temperature by physiological means and having a body temperature virtually identical to that of their environment [54]. Consequently, increasing global temperatures can affect them by altering physiological functions such as thermal tolerance, growth, metabolism, food consumption, reproductive success and the capacity to maintain internal homeostasis in the face of a variable external environment [56]. Fish populations that experience changing thermal regimes may increase or decrease in abundance, experience range expansions or contractions, or be threatened with extinction [54]. The increase in temperature observed by fishermen can illustrate the decline of the population of these species. In addition, due to the preference of these species for vegetation or forest areas, agriculture expansion and pesticide use along watersheds may pose a threat to their habitat and populations. Agriculture and pesticide use along watersheds were listed by the fishermen in this study among anthropogenic activities threatening the Ivorian freshwater ecosystem. Most of the species listed are among the main species of the Ivorian fishery [57]. Overfishing has been reported by fishermen as one of the threats to the biodiversity of Ivorian freshwater fish. Thus, it may represent one of the probable causes of the rarity of these species. According to the result of a recent study on the assessment of the conservation status and the vulnerability of the ichthyofauna of the Bandama River in Côte d'Ivoire, species such as Lates niloticus, Labeo coubie, Heterotis niloticus and Sarotherodon galilaeus, despite their status as species of least concern, were assessed as vulnerable to fishing pressure [17]. Many other studies carried out on the African continent have reported that these species are locally threatened by many humans' actions and climate change. Lates niloticus is reported to suffer from over-exploitation in much of its native range, and to a lesser extent, pollution [58]. Heterotis niloticus and Parachanna obscura are also affected by overfishing in central and western Africa respectively, where they represent commercially important fish species [47,59]. Malapterurus electricus, Sarotherodon galilaeus, Tilapia zillii and Labeo coubie are threatened by dam constructions, water pollution, groundwater extraction and drought in northern Africa, whereas Malapterurus electricus and Sarotherodon galilaeus are additionally affected by overfishing in eastern Africa [51,53,52,48].

Conclusion and perspectives
Côte d'Ivoire is a climatically diverse country with a great diversity of freshwater ecosystems.
The results of this study show good awareness among local fishermen of climate change and its drivers. That climatic awareness represents a positive prerequisite for the implementation of measures toward climate mitigation and population adaptation. Fish abundance reduction was reported as a consequence of climate change and several anthropogenic activities. In response to this reduction in fish quantity, the government must ensure the strict application of policies and regulations in force in the fisheries sector, through the establishment of local management and monitoring committees. These should be done by involving fishermen associations (or cooperatives) in the process of decision making, their implementation, and monitoring. This will improve the effectiveness of actions and contribute to the sustainable management of fishing resources. In addition, to enable fishing communities to adapt to the decline of fish, they need to be trained to diversify their sources of income. For example, training them in agriculture (livestock, aquaculture, etc.) or other activities such as mechanics, commerce, electricity, etc. This will not only strengthen their adaptive capacities but also reduce excessive pressures on aquatic ecosystems. All these measures should be implemented through fishermen associations or cooperatives. In Côte d'Ivoire, fishermen are mainly organized in cooperatives or associations [60]. The roles of these are most often to contribute to food security, poverty eradication, promotion of collective action, empowerment of fishermen to respond to environmental and socio-economic changes, and sharing of responsibility in the practice of responsible fishing, but also to have a good voice in the social-economic process. However, the movement of cooperatives or associations in the fisheries sector is characterised by a lack of dynamism in Côte d'Ivoire. Those function with difficulty, thus giving the impression that there are no existing fisheries actors in Côte d'Ivoire [60]. Thus, the government should first revitalise the associations or cooperatives by providing financial supports, but also train them on their roles and duties.
The information provided by fishermen constitutes basic information on the evolutions and the main factors impacting fish abundance in Ivory Coast. These are useful for directing and approving adaptation and management strategies of fish resources. These results should be supported by further studies that will focus on quantifying the level of decrease in fish quantity, the contribution of each threat in this decline, the level of correlation between these threats, the identification of the most impactful threats, and the assessment of the vulnerability and the already existing local adaptation strategies in Ivorian local fishermen communities. These will give a clear view on which management strategies will be more appropriate for the sustainability of fish resources and how to improve the existing adaptation strategies.
Fish species listed by fishermen as being threatened are economically among the most important fish species for the livelihood of many African countries, especially in Côte d'Ivoire. The reassessment of the local status of these species using IUCN criteria is needed to guide management and conservation policies. Furthermore, more research studies are needed to assess and quantify the vulnerability of these species to climate change and human activities. The assessment of the distribution, genetic diversity and population dynamics of these species will also be necessary to develop appropriate management and conservation policies.