Hue and cry for Fritillaria cirrhosa D.Don, a threatened medicinal plant in the Western Himalaya

The unique Himalayan ecosystems are repositories to the wild populations of diverse flora and fauna. The high value medicinal and aromatic plant species (MAPs) are an example of the same. Since time immemorial, these MAPs have been traditionally used by the local inhabitants and have eventually developed a high market value all over the world. Increasing market demand engenders over-extraction of species, unsustainable collection further catalyses decline in wild populations. The current communication raises high conservation concern on the rapid population decline of Fritillaria cirrhosa D.Don in the Western Himalaya. Harvested and traded with a new trade name i.e., ‘Jangli lehsun’ probably to disguise common Allium species, the species is facing tremendous decline in wild populations due to its illegal harvesting and trade in Himachal Pradesh. Further, F. cirrhosa faces threat due to unorganized, over-extraction, unsustainable and premature harvesting of the bulbs, coupled with illegal hidden markets functioning parallelly. Considering that this valuable species is under multiple threats being a medicinally important plant, priority should be given for its conservation through in-situ such as identification of medicinal plant conservation areas and ex-situ methods for its propagation and multiplication. Further, to ensure the long-term conservation of Fritillaria cirrhosa, prioritized conservation strategies such as strengthening of the Biodiversity Management Committees, capacity building through awareness programs for the key stakeholders and sustainable harvesting would be the practical solution.

of MAPs along with isolation of these ecosystems for centuries in terms of culture, geography, politics and physiography has resulted in a unique health care system [2]. Earlier, very little was known and documented about traditional medicinal system i.e., the Tibetan Medicinal System (TMS) or Amchi system of medicine (Sowa-Rigpa) [3], with globalization and road accessibility that is no longer the case. These regions too, are no longer cut off from global societies resulting in cultural and social transformations [4]. Meager opportunities for employment and fewer alternative sources of sustenance force the local inhabitants towards over-exploitation of the existing natural resources, aggravating their degradation [4]. One such example is the steadily increasing pressure on the medicinal plant populations from the wild. These ecosystems are naturally gifted with MAPs growing in wild even more than in cultivation and, form an important source of sustenance and profit making for the locals however, unsustainable harvesting has resulted in rapid decline of the wild populations of these species. For instance, the annual demand of some important MAPs such as, Gentiana kurroo Royle and Picrorhiza kurroa Royle ex Benth. is almost 5000 tonnes each and that of Aconitum heterophyllum Wall. ex Royle is 1000 tonnes, where as the supply of these species is only 100 tonnes per year [5]. According to Walter and Gillett [6], imbalance between demand and supply, unfortunately is one of the reasons for the documented extinction of more than 19 plant species in the wild from India, 41 species suspected of having recently become extinct, 102 species are believed to have moved to higher category of rarity, 152 species in threat of extinction if the unsustainable extraction continues and, about 251 species under high risk and only restricted to limited areas. Owing to high demand, small or restricted population, several MAPs have undergone rapid decline in wild population from the Indian Himalayan Region (IHR), for example, rampant collection of Trillium govanianum Wall. ex D.Don (Nagchatri) from the Western Himalaya has grown tremendously in the past few years. It is being sold at rates approximately INR 1000 to 1500 kg −1 dry weight. The unregulated trade flourishes despite the ban by the Government of Himachal Pradesh on transportation of the plant material of Nagchatri [7]. Another well-known insect fungus commonly known as Keedajadi or Yartsagunbu (Ophiocordyceps sinensis syn. Cordyceps sinensis) with multiple medicinal uses, has received high attention in terms of demand and price in the Himalayan region, especially Uttarakhand, Nepal and China. The collection serves as a lucrative livelihood opportunity to the local collectors however, unrestrained extraction coupled with livelihood dependency hasled to depletion of Keedajadi in the wild [8]. Similarly, the reported population of Indian spikenard or Indian Valerian (Nardostachys jatamansi (D.Don) DC.) has declined 70-80% in the wild from IHR [9]. Whereas, Salampanja or Hathajadi (Dactylorhiza hatagirea D.Don Soó) has created high demand in the Indian and International markets owing to its high medicinal and edible value [10] with annual demand reaching around 5000 tonnes [11]. The regeneration capacity of this high value orchid is rather poor due to pollinator specificity and requirement of mycorrhizal association [12] therefore, over extraction from the wild poses a serious threat.

Frtillaria cirrhosa D.Don: a high conservation concern
The current communication highlights sudden spurt in demand and market trade of yet another MAP, Frtillaria cirrhosa (syn. Fritillaria royeli Hook.) with little knowledge of its usage as well as market or end users is a cause of grave concern in its distribution range in the Western Himalaya. Recently, as a part of Wildlife Institute of India's research project on medicinal and aromatic plants in Himachal Pradesh with logistics support from the Himachal Pradesh Forest Department (HPFD) and funds received from the United Nations Development Program and Global Environment Facility, the authors after a span of more than 2 years (2018-2021) report drastic decline in the population of F. cirrhosa in the Lahaul and Pangi landscapes of Himachal Pradesh (Fig. 1). Pangi spreads over 1601 km 2 area is positioned approximately between 32°11′30″-33°13′06″ N and 75°45′-77°03′33″ E with 54 inhabited villages. Lahaul lies between 32°61′92″ N and 77°37′84″ E with 132 villages. These landscapes are located between the Dhauladhar-Pir Panjal and Zanskar ranges in Himachal Pradesh in the upper catchment of Chandrabhaga (Chenab) that forms a transition zone between the Greater and Trans-Himalaya.
Commonly known as Kakoli, F. cirrhosa is an Asian species of herbaceous plant belonging to Liliaceae (lily) family (Fig. 2). The name Fritillaria derived from the Latin 'fritillus' meaning dice-box, possibly refers to the checkered pattern on the flowers. It is mainly distributed in Pakistan, China, India, Nepal, Bhutan and Myanmar. In India, it shows distribution in the states of Uttarakhand, Himachal Pradesh and Jammu & Kashmir [13] which grows mainly on rocky and grassy slopes in alpine and sub-alpine regions amidst shrubberies such as Lonicera spp., Rosa spp. and Salix spp. at an elevation ranging from 3000 to 4200 m [14]. It generally flowers between May-July and fruits from September-October. This herbaceous plant has yellowish green to brownish purple bell-shaped solitary flowers, leaves are linear, lanceolate and long pointed. Also known as yellow Himalayan fritillary, F. cirrhosa forms an important component of a polyherbal formulation of eight herbs (asthavarga) [15]. The sub-terranean bulb is used as febrifuge, galactagogue, haemostatic, expectorant, aphrodosiac, anti-rheumatic, spermatogenic and tonic, the rhizome is useful in excessive thirst, rheumatic pain and heamatemesis [16][17][18][19][20][21].
Based on semi-structured questionnaire surveys (n = 15), individual interviews, group discussions, scrutiny of export data obtained from respective forest divisions and visits to villages (29) and local markets (06) in Himachal Pradesh, the current study reports severe threat to the species predominantly due to premature collection of bulbs, over exploitation, unorganized harvesting and illegal trade in the landscapes. Harvested and traded with a new trade name i.e., 'Jangli lehsun' probably to disguise common Allium species, the species is facing tremendous decline in wild populations due to its illegal harvesting and trade in the landscapes. Unfortunately, no information on the medicinal use has been reported from the local inhabitants however, the species sells in the market like a hot cake. Enthusiastic collection of premature bulbs during July (instead of September-October on bulbs maturity) has resulted in the absence of perennating buds for the next growing season. For instance, premature bulbs collected by a local was observed at the site with more than 5 kg costing INR 60,000 in 2019 (Fig. 3). Unfortunately, contrary to other MAPs, there is no harvesting and collection protocol developed for this species. This has created ambiguity in terms of the ultimate market and actual use of Jangli lehsun in the region. In order to reduce illegal harvesting of Jangli lehsun, HPFD issues permits to local plant collectors with permit fee of INR 10,000 quintal −1 ; however, illegal trade continues to flourish owing to the hidden markets functioning parallelly. Further, as one moves up in the value addition hierarchy, the intermediaries and middlemen receive higher returns than the collectors at the grassroot level in the market chain [22]. The price along the trade chain was also studied from the production level in landscape villages, small aggregation markets in towns and large mandis (markets) in cities. The result illustrated an increase in the price of MAPs as it moves from village to city (Fig. 4). Although, extensive field-based study on available growing stock and population status of F. cirrhosa would be essential in the landscapes, the present study concludes that the prevailing unselective and unsustainable harvesting may lead to the regional extinction of F. cirrhosa in its natural habitats in the Western Himalaya.
Owing to its population reduction by 58-77% during the last 20-30 years [13], the species has been assessed using IUCN Red List Criteria and has been listed as 'Critically Endangered' in Uttarakhand and 'Endangered' in Himachal Pradesh and Jammu and Kashmir [23]. The market demand of this species is increasing while the supply is gradually decreasing [24]. Thus, extensive regional assessments of MAPs in Himachal Pradesh [25,26] have also categorized this species as 'Endangered' , hence, restoring wild populations is crucial.

Market and trade
Frtillaria cirrhosa is one of the 18 species that are actively traded throughout the world and constitutes large scale industry; worth 400 million US dollar per annum demand in China [27]. In India, market value of dry bulbs is approximately 10,000-15,000 kg −1 INR in local markets [28]. Although, Frtillaria cirrhosa is prohibited in India for export if the plants are collected from the wild. Raw drug obtained from cultivated material can however, be exported. According to Goraya and Ved [29], trade per annum and price of F. cirrhosa was found to be < 10 MT with price ranging from 1200 to 6000 kg −1 respectively. As per the HPFD during 2017 to 2021, the total estimated volume of the exported quantity of Fritillaria cirrhosa was 291.16 quintals in the Pangi landscape [22] (Table 1). Further, the average market price of F. cirrhosa was recorded between 9666.66 ± 881.92 to 17,666.66 ± 1452.97 (Rs/kg ± SD) in major markets viz., Udaipur (Lahaul), Killar (Pangi), Keylong, Manali and Amritsar [22] (Fig. 4). According to Goraya and Ved [29], Jangli lehsun witnessed a sudden spurt between 2009-10 and 2014-15 in its distributional range in the states of Himachal Pradesh, Jammu & Kashmir and Uttarakhand for its bulbs. The wild populations of F. cirrhosa have succumbed to this high annual harvesting pressure pushing these species towards possible extinction.

Conclusion
High demand of F. cirrhosa, makes this species one of the most intensively harvested alpine Himalayan medicinal bulbs. Due to demand exceeding supply, the price of F. cirrhosa bulbs has increased dramatically. For example, the price of wild harvested F. cirrhosa bulbs increased over nine-fold, from the equivalent of US$60 in 2002 to US$560 kg −1 in 2017 [20]. Owing to its strict habitat requirements, domestication and cultivation is extremely difficult, therefore, majority of F. cirrhosa is still gathered from the wild. Thus, to reduce pressure on the wild populations, designing sustainable harvesting and collection protocols, and development of tools and techniques for its cultivation is the need of the hour. As this valuable species is under multiple threats due to its high demand, market and intensive grazing is a problem as Fritillaria plants are eaten, reducing seed set and germination from seed. Coupled to slow rates of vegetative reproduction from bulbs, grazing has resulted in a significant population size decline [20], the present investigation concludes that sustainable management coupled with organized collection and utilization would be the practical solution for the conservation of F. cirrhosa. Overexploitation of wild populations is not the only concern when it comes to unsustainable harvest, but may also have a ripple-effect on other Fritillaria species. Therefore, additional compounding factors influencing the sustainable harvest and conservation of F. cirrhosa populations need to be taken into account [20]. Subsequently, strengthening of Biodiversity Management Committee for establishing inventories on the existing natural resources of the region and spreading awareness on the dwindling populations can be the way forward. Identifying and building the capacities of stakeholders such as Forest Department staff, local collectors, amchis or traditional healers and medicinal plant traders in the process of conservation can help in community based natural resource management. Introduction of state of art technology in cultivation, harvesting and storage of MAPs in general and Jangli lehsun specifically, would also help to avail the best rates at the grass root level. Thus, considering that F. cirrhosa is under multiple threats being a high value medicinal plant, this species should be given priority for conservation through establishment of in-situ such as establishment of Medicinal Plant Conservation Areas and ex-situ methods as potential source areas.