Abstract
Aconite and cinnamon can treat many diseases. The study aimed to assess the effectiveness and mechanism of aconite, cinnamon, and aconite-cinnamon in bone formation-bone resorption. The co-culture system was treated with serum containing aconite, cinnamon, and aconite-cinnamon, and the effects on osteoblast and osteoclast differentiation were evaluated through ALP activity, ALP staining, alizarin red staining, TRAP activity detection, TRAP staining, and F-actin staining. The study also examined the effects of aconite-cinnamon on intercellular factors using ELISA kits, and the effects on different genes were detected through RT-qPCR and Western blotting. Results showed that aconite-cinnamon improved osteoblast differentiation and activity, reduced the release of RANKL, inhibited osteoclast differentiation and activity, and activated the AMPK/SIRT1 signaling pathway in both osteoblasts and osteoclasts. Therefore, aconite-cinnamon can improve osteogenic differentiation activity and reducing osteoclast differentiation and activity, while activating the AMPK/SIRT1 signaling pathway in both osteoblasts and osteoclasts.
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Data Availability
All data generated or analyzed during this study are included in this published article. The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
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Acknowledgements
We appreciate the support from the Public Research Platform of the Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University.
Funding
This work was supported by National Natural Science Foundation Youth Project of China (No. 82004247), Zhejiang Provincial Natural Science Foundation of China under Grant No. LQ21H270002, and Natural Science Foundation of Chongqing No. CSTB2022NSCQ-MSX0652.
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LQ, LZ, and QZ participated in the design of the experiment; LY and PS did experiments and data analysis; the draft of the manuscript was written by LY. All the authors read and approved the final manuscript. The authors declare that all data were generated in-house and that no paper mill was used.
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Yao, L., Zhang, Q., Sun, P. et al. Effect of Cinnamon and Aconite on Bone Formation-Bone Absorption Coupling in Bone Microenvironment. Rev. Bras. Farmacogn. 34, 511–521 (2024). https://doi.org/10.1007/s43450-023-00504-5
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DOI: https://doi.org/10.1007/s43450-023-00504-5