Abstract
Type 2 diabetes mellitus is accompanied by hyperglycaemia, liver dysfunction and intestinal dysbiosis. Bioactive peptides, as functional food, ameliorate type 2 diabetes mellitus by preventing oxidative damage to the liver. Bacillus amyloliquefaciens fmb50 was used in our earlier studies to create the lipopeptide surfactin, which was shown to alleviate type 2 diabetes mellitus by regulating the intestinal flora. In this study, high-fat diet/streptozotocin-induced type 2 diabetes mellitus mice were treated with surfactin from B. amyloliquefaciens fmb50 for 12 weeks. Faecal metabolomics profiling was analysed to explore the antihyperglycaemic mechanism of surfactin in type 2 diabetes mellitus mice. The results suggested that 32 differential metabolites were identified from faeces, suggesting that surfactin administration partly exerted an antihyperglycaemic effect in type 2 diabetes mellitus mice. Finally, surfactin was shown in further experiments to lower serum and liver triacylglycerides, reduce liver fat accumulation, inhibit adipocyte hypertrophy and reverse liver, pancreas, adipose tissue and colon tissue damage in type 2 diabetes mellitus mice. These findings suggest that early surfactin supplementation might delay the development of type 2 diabetes mellitus.
Graphical abstract
Potential mechanism by which surfactin relieves T2DM. Green arrows pointing down indicate decreased parameters or metabolites in the M group, and green arrows pointing up indicate increased parameters or metabolites in the M group. Red arrows pointing down indicate decreased parameters or metabolites in the T2DM group, and red arrows indicate pointing up increased parameters or metabolites in the T2DM group.
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All relevant data have been included in the manuscript and supplementary file.
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This study was supported by the National Natural Science Foundation of China (No. 32072182).
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Among the authors in the list, XC and JH mainly took charge of the study design and in the preparation and drafting of the manuscript. HZ and YL contributed to the data collection and analysis. FM, ZL, YL and FL played the guiding role in revising the manuscript.
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Xiaoyu Chen and Jiaming Huang are co-first authors.
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Chen, X., Huang, J., Zhao, H. et al. Faecal Untargeted Metabolomics Analysis Elucidates the Antihyperglycaemic Effect of Surfactin in High-Fat Diet/Streptozotocin-Induced Type 2 Diabetic Mice. Rev. Bras. Farmacogn. 34, 407–419 (2024). https://doi.org/10.1007/s43450-023-00499-z
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DOI: https://doi.org/10.1007/s43450-023-00499-z