Abstract
Leishmaniasis is a parasitic disease with a limited arsenal of drugs for therapeutical care, and the available treatments present a variety of side effects, demonstrating the need for alternative therapies. Handroanthus impetiginosus (Mart. ex DC.) Mattos, Bignoniaceae (ipê-roxo), is recognized for showing a wide number of biological activities. Hence, this paper aims to investigate the extracts of different parts of H. impetiginosus and their antileishmanial activity through in vitro assays. The evaluation of the antileishmanial activity of the leaf and seed ethanolic extracts showed the best results after 24 h with IC50 4.49 and 2.24 µg/ml, respectively. Already, amphotericin B (positive control) showed an IC50 of 0.012 µg/ml. The chemical composition revealed metabolites such as flavonoids, triterpenoids, iridoid glycosides, hydroxyanthraquinones, naphtofurans, naphthoquinones, coumaric acid derivatives, lignans, and phenolic compounds. The results obtained in this paper contribute to the scientific knowledge regarding the chemical profile and biological potential of ipê-roxo.
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References
Araújo MV, David CC, Clementino Neto J, Oliveira LAPL, Silva KCJ, Dos Santos JM, Silva JKS, Brandão VBCA, Silva TMS, Camara CA, Alexandre-Moreira MS (2017) Evaluation on the leishmanicidal activity of 2-N, N’- dialkylamino-1,4- naphtoquinone derivatives. Exp Parasitol 176:46–51. https://doi.org/10.1016/j.exppara.2017.02.004
Costa EVS, Brígido HPC, E Silva JVS, Coelho-Ferreira MR, Brandão GC, Dolabela MF (2017) leishmanicidal activity of Handroanthus serratifolium (Vahl) S. Grose (Bignoniaceae). Evid based Complement Altern Med 2017:8074275. https://doi.org/10.1155/2017/8074275.
de Carvalho BL, Figueiredo MR (2022) Bioprospecção de atividade anticâncer dos gêneros Garcinia e Clusia: uma breve revisão. Rev Fitos 16:247–266
Draize JH, Woodard G, Calvery HO (1944) Method for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther 82:338–390
Forbes VE, Forbes TL (1994) Ecotoxicology in theory and practice. Champman and Hall.
Gomes CL, Sales VDAW, de Melo CG, da Silva RMF, Nishimura RHV, Rolim LA, Neto PJR (2021) Beta-lapachone: natural occurrence, physicochemical properties, biological activities, toxicity and synthesis. Phytochemistry 186:112713. https://doi.org/10.1016/j.phytochem.2021.112713
Gontijo B, Carvalho MLR (2003) Leishmaniose tegumentar americana. Rev Bras Med Trop 36:71–80
Gonzalez-Coloma A, Reina M, Sáenz C, Lacret R, Mesia R, Arán V, Sanz J, Martínez-Díaz RZ (2012) Leishmanicidal, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants. Parasitol Res 110:1381–1392. https://doi.org/10.1007/s00436-011-2638-3
Lorenzi H (2002) Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil, 4ed. Nova Odessa: Editora Plantarum.
Martins L, Lago AA, Cicero SM (2011) Qualidade fisiológica de sementes de Tabebuia avellanedae e Tabebuia impetiginosa submetidas à ultra-secagem. Rev Bras Sementes 33:626–634
Moraes PSP, Carvalho CS, Chaves DA, Sousa MAM, Lemes SR, Araujo LA, Mrué F, Melo-Reis PR (2016) Avaliação da atividade angiogênica da solução aquosa da Tabebuia impetiginosa (Ipê-roxo). Revista EVS 43:47–53
Nakamura CV, Santos AO, Vendrametto MC, Luize PS, Dias Filho BP, Cortez DAG, Ueda-Nakamura T (2006) Atividade antileishmania do extrato hidroalcoólico e de frações obtidas de folhas de Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. Rev Bras Farmacogn 16:61–66. https://doi.org/10.1590/S0102-695X2006000100011
Neusaumer S, Bonnabry P, Veuthey JL, Fleury-Souverain S (2011) Analysis of anticancer drugs: a review. Talanta 85:2265–2289. https://doi.org/10.1016/j.talanta.2011.08.034
Newman DJ (2017) The influence of Brazilian biodiversity on searching for human use pharmaceuticals. J Braz Chem Soc 28:402–414. https://doi.org/10.21577/0103-5053.20160225
Osorio E, Gj A, Jiménez N, Alzate F, Ruiz G, Guitiérrez D, Ma P, Giménez A, Robledo S (2007) Antiprotozoal and citotoxic activities in vitro of Colombian Annonaceae. J Ethnopharmacol 111:630–635. https://doi.org/10.1016/j.jep.2007.01.015
Pereira JBA, Rodrigues MM, Morais IR, Vieira CRS, Sampaio JPM, Moura MG, Damasceno MFM, Silva JN, Calou IBF, Deus FA, Peron AP, Abreu MC, Militão GCG, Ferreira PMP (2015) O papel terapêutico do Programa Farmácia Viva e das plantas medicinais. Rev Bras Pl Med 17:55–561. https://doi.org/10.1590/1983-084X/14_008
Pereira PS, Oliveira C, Maia AJ, Vega-Gomez MC, Rolón M, Coronel C, Duarte AE, Coutinho H, Siyadatpanah A, Norouzi R, Sadati S, Wilairatana P, Silva TG (2022) Evaluation of the in vitro antiparasitic effect of the essential oil of Cymbopogon winterianus and its chemical composition analysis. Molecules 27:2753. https://doi.org/10.3390/molecules27092753
Pinto AV, Castro SL (2009) The trypanocidal activity of naphthoquinones: a review. Molecules 14:4570–4590. https://doi.org/10.3390/molecules14114570
Pradhan S, Schwartz R, Patil A, Grabbe S, Goldust M (2022) Treatment options for leishmaniasis. Clin Exp Dermatol 47:516–521. https://doi.org/10.1111/ced.14919
Sen R, Chatterjee M (2011) Plant derived therapeutics for the treatment of leishmaniasis. Phytomedicine 18:1056–1069. https://doi.org/10.1016/j.phymed.2011.03.004
Silva MLA, Lucarini R, Santos FF, Martins CHG, Pauletti PM, Januario AH, Santos MFC, Cunha WR (2021) Hypoglycemic effect of rosmarinic acid-rich infusion (RosCE) from Origanum vulgare in alloxan-induced diabetic rats. Nat Prod Res. https://doi.org/10.1080/14786419.2021.1990282
Soltani S, Foroutan M, Hezarian M, Afshari H, Kahvaz MS (2019) Cutaneous leishmaniasis: an epidemiological study in southwest of Iran. J Parasit Dis 43:190–197. https://doi.org/10.1007/s12639-018-1073-0
Suffness M, Pezzuto JM (1990) Assays related to cancer drug discovery. In: Hostettmann K (ed) Methods in plant biochemistry: assays for bioactivity, 6th edn. Academic Press, London, pp 71–33
Tavares G, Mendonça D, Lage DP, Granato J, Ottoni FM, Ludolf F, Chávez-Fumagalli MA, Duarte MC, Tavares C, Alves RJ, Coimbra ES, Coelho E (2018) Antileishmanial activity, cytotoxicity and mechanism of action of clioquinol against Leishmania infantum and Leishmania amazonensis species. Basic Clin Pharmacol Toxicol 123:236–246. https://doi.org/10.1111/bcpt.12990
Ullah N, Nadhman A, Siddiq S, Mehwish S, Islam A, Jafri L, Hamayu M (2016) Plants as leishmanicidal agents: current scenario. Phytother Res 30:1905–1925. https://doi.org/10.1002/ptr.5710
Acknowledgements
The authors thank the National Council for Science and Technology (Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq) and Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES) for financial support and the researchers from the Natural Products and Organic Synthesis Research Group (GEAPS-CNPq-UFES). The authors also thank the researchers Jairo K. Bastos and Nicoli Dias Oliveira.
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Acquisition of samples: DLM, AAM, PSSRC, and SGA; chemical characterization analysis of UPLC-ESI-qTOF experimental data: AKN, MFCS, OSC, and CPFS; biological activity analysis and interpretation of experimental data: WRC, MLAS, and LGM; drafting the article and revising it critically for important intellectual content: AHJ and PMP. All of the authors have read and approved the final submission.
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Mariano, R.L., Montagnini, D.L., Manuquian, H.A. et al. Antileishmanial Activity and Chemical Composition of Hydroalcoholic Extracts from Different Parts of Handroanthus impetiginosus (Ipê-Roxo). Rev. Bras. Farmacogn. 32, 851–857 (2022). https://doi.org/10.1007/s43450-022-00313-2
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DOI: https://doi.org/10.1007/s43450-022-00313-2