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Agomelatine prevents indomethacin-induced gastric ulcer in rats

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Abstract

Background

Gastric ulcer is a very common gastrointestinal disease that may be dangerous and even may lead to death. The current study was conducted to detect the prophylactic effects of agomelatine on indomethacin-induced gastric ulcer.

Methods

In this study, a total of 5 groups were created as the sham, ulcer, omeprazole, agomelatine 1 mg/kg and agomelatine 5 mg/kg groups. The effects of agomelatine on indomethacin-induced gastric injury were investigated. Total antioxidant and oxidant levels; the oxidant parameters like oxidative stress index and the inflammation markers such as tumor necrosis factor-α, interleukin-1β, interleukin-6 and interleukin-10 levels in stomach tissue were determined by ELISA. In addition, the gastric mucosal injury occurred in stomach wall was examined with histopathological methods.

Results

While the levels of the inflammatory markers, total oxidant status and oxidative stress index increased at an obvious level especially in the indomethacin group, the total antioxidant status levels decreased. It was observed that these parameters were improved at a significant level in agomelatine 1 mg/kg and agomelatine 5 mg/kg groups when compared to ulcer group; and the results were similar to omeprazole group. It was also observed that our histopathological findings were consistent with all our other results.

Conclusions

The results of this study showed that agomelatine usage in indomethacin-induced gastric ulcer model provides beneficial results.

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Abbreviations

HCl:

Hydrochloric acid

NSAID:

Nonsteroidal anti-inflammatory drug

ROS:

Reactive oxygen species

MT1 and MT2:

Melatonin receptor 1 and 2

AGO:

Agomelatine

GI:

Gastrointestinal

DMOS:

Dimethyl sulfoxide

TAS:

Total antioxidant status

TOS:

Total oxidant status

IL-1β:

Interleukin 1-beta

IL-6:

Interleukin-6

IL-10:

Interleukin-10

TNF-α:

Tumor necrosis factor-alfa

OSI:

Oxidative stress index

NF-κB:

Nuclear factor kappa B

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Acknowledgements

This study was supported by the Department of Scientific Research Projects of Atatürk University (Project No. 2016/51).

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Yozgat Bozok University, Yozgat, 66200, Turkey

    Ersen Eraslan

  2. Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Ayhan Tanyeli & Mustafa Can Güler

  3. Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Nezahat Kurt

  4. Department of Histology and Embryology, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Zeliha Yetim

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  1. Ersen Eraslan
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Eraslan, E., Tanyeli, A., Güler, M.C. et al. Agomelatine prevents indomethacin-induced gastric ulcer in rats. Pharmacol. Rep 72, 984–991 (2020). https://doi.org/10.1007/s43440-019-00049-2

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