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TSGA10 as a Potential Key Factor in the Process of Spermatid Differentiation/Maturation: Deciphering Its Association with Autophagy Pathway

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Abstract

Testis-specific gene antigen 10 (TSGA10) plays an important role in spermatogenesis. However, the exact TSGA10 role and its relationship with the autophagy pathway in the process of spermatids differentiation/maturation is still not clear. Therefore, the present study evaluates the role of TSGA10 gene in the spermatid differentiation/maturation through its effect on autophagy and explores possible underlying pathway(s). Sperm samples from patients with teratospermia were collected. The mRNA and protein level of TSGA10 in these samples were assessed by real-time PCR and western blotting. Using the ingenuity pathway analysis (IPA) software, the gene network and interactions of TSGA10 involved in sperm maturation and autophagy were investigated. Based on these analyses, the expression levels of identified genes in patient’s samples and healthy controls were further evaluated. Moreover, using flow cytometry analysis, the levels of reactive oxygen species (ROS( production in teratospermic sperm samples were evaluated. The results showed that the expression levels of TSGA10 mRNA and protein decreased significantly in the teratospermic patients compared to controls (P < 0.05). Moreover, a significant reduction in the expression of the important genes involved in sperm maturation and autophagy was observed (P < 0.05). Also, the levels of ROS production in teratospermic sperm samples were shown to be significantly higher compared to those in normal sperms (P < 0.05). Our findings provide new evidence that simultaneous decrease in TSGA10 and autophagy beside the increased level of ROS production in sperm cells might be associated with the abnormalities in the spermatids differentiation/maturation and the formation of sperms with abnormal morphology.

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Funding

This study has been supported by Kermanshah University of Medical Sciences, Kermanshah, Iran (grant number: 96118).

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Authors and Affiliations

  1. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Rezvan Asgari, Davood Rezazadeh & Kamran Mansouri

  2. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mitra Bakhtiari

  3. Translational Type 1 Diabetes Research, Department of Clinical Research, Steno Diabetes Center Copenhagen, Gentofte, Denmark

    Reza Yarani

  4. Infertility Treatment Research Center, Moatazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Farzaneh Esmaeili

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  1. Rezvan Asgari
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  2. Mitra Bakhtiari
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Correspondence to Kamran Mansouri.

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The present study has been approved by the Ethics Committee of the Kermanshah University of Medical Sciences (IR.KUMS.REC.1396.1).

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Asgari, R., Bakhtiari, M., Rezazadeh, D. et al. TSGA10 as a Potential Key Factor in the Process of Spermatid Differentiation/Maturation: Deciphering Its Association with Autophagy Pathway. Reprod. Sci. 28, 3228–3240 (2021). https://doi.org/10.1007/s43032-021-00648-6

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