A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including plants. However, screening edited alleles, particularly those with multiplex editing, from herbicide- or antibiotic-resistant transgenic plants and segregating out the Cas9 transgene represent two laborious processes. Current solutions to facilitate these processes rely on different selection markers. Here, by taking advantage of the opposite functions of a d-amino acid oxidase (DAO) in detoxifying d-serine and in metabolizing non-toxic d-valine to a cytotoxic product, we develop a DAO-based selection system that simultaneously enables the enrichment of multigene edited alleles and elimination of Cas9-containing progeny in Arabidopsis thaliana. Among five DAOs tested in Escherichia coli, the one encoded by Trigonopsis variabilis (TvDAO) could confer slightly stronger d-serine resistance than other homologs. Transgenic expression of TvDAO in Arabidopsis allowed a clear distinction between transgenic and non-transgenic plants in both d-serine-conditioned positive selection and d-valine-conditioned negative selection. As a proof of concept, we combined CRISPR-induced single-strand annealing repair of a dead TvDAO with d-serine-based positive selection to help identify transgenic plants with multiplex editing, where d-serine-resistant plants exhibited considerably higher co-editing frequencies at three endogenous target genes than those selected by hygromycin. Subsequently, d-valine-based negative selection successfully removed Cas9 and TvDAO transgenes from the survival offspring carrying inherited mutations. Collectively, this work provides a novel strategy to ease CRISPR mutant identification and Cas9 transgene elimination using a single selection marker, which promises more efficient and simplified multiplex CRISPR editing in plants. Supplementary Information The online version contains supplementary material available at 10.1007/s42994-023-00132-6.

thaliana, Cas9 overexpression by a strong constitutive promoter (e.g., 35S promoter) often results in chimeric mutations in the T 1 generation (Feng et al. 2014;Tsutsui and Higashiyama 2017;Wolabu et al. 2020).In contrast, using an egg cell-specific promoter to drive Cas9 expression can produce nonchimeric (that is, heterozygous, homozygous or biallelic) T 1 mutants, although with a relatively lower efficiency (Wang et al. 2015).The identification of CRISPR-edited transgenic plants routinely requires PCR-based genotyping and/or target amplicon sequencing, which is time-consuming and costly.Therefore, strategies to enrich CRISPR-edited plants during mutant screening are highly desirable, particularly in the case of multiplex editing.Recently, surrogate systems based on the restoration of a defective hygromycin resistance gene (HygR) have been developed to confer effective enrichment of rice mutant plants in CRISPR editing (Tian et al. 2023), base editing (Xu et al. 2020a), and prime editing (Xu et al. 2020b).CRISPR-mediated disruption of MAR1, an endogenous transporter gene, has been demonstrated to facilitate co-selection of target gene editing in Arabidopsis and tomato (Rinne et al. 2021).A visible selection system has also been established in Arabidopsis to enrich CRISPR-induced mutants in the T 1 generation, which is based on a glabrous phenotype caused by mutating the GLABRA2 gene that is involved in trichome formation (Kong et al. 2021).
On the other hand, the retention of Cas9 transgene after target gene knockout can significantly increase the risk of off-target editing (Fu et al. 2013;Zhang et al. 2018;Cullot et al. 2019).Moreover, in transgenic complementation experiments for validating the mutant phenotype and target gene correlation, it is necessary to eliminate the Cas9 before the target gene is re-introduced into the edited mutant plant.Furthermore, in CRISPR-mediated molecular breeding, the inclusion of the Cas9 transgene in crops raises regulatory concerns.Although the Cas9 transgene can be eliminated through genetic segregation, the process of screening nontransgenic progeny with desired mutations is laborious.Therefore, several strategies have been developed to facilitate the isolation of Cas9-free mutant plants (He and Zhao 2019).One approach for eliminating Cas9 from genome-edited plants involves RNA interference-induced herbicide susceptibility, which allows the survival of Cas9-free plants (Lu et al. 2017).Alternatively, nontransgenic seeds or plants could be visually distinguished from transgenic ones by integrating a transgene giving rise to detectable fluorescence (Gao et al. 2016;Wang and Chen 2020), early flowering (Liu et al. 2019;Lao et al. 2023), or anthocyanin accumulation (Liu et al. 2019).Although He and colleagues have created an ingenious strategy, called TKC (Transgene Killer CRISPR), enabling self-elimination of the CRISPR construct after targeted gene editing in rice (He et al. 2018), this approach cannot be applied to Arabidopsis that is transformed by the floral dip method (Clough and Bent 1998) rather than tissue culture.Of note, to date, no strategy has been developed to enable both the enrichment of CRISPR-edited mutants and subsequent elimination of the Cas9 transgene from the mutant offspring.
D-amino acid oxidases (DAOs) are specialized enzymes for D-amino acid metabolism, which are encoded by many eukaryotes but not plants (Pilone 2000).Interestingly, D-serine at high concentrations is toxic to plants but can be detoxified by transgenically expressed DAOs (Erikson et al. 2004;Lim et al. 2007;Gisby et al. 2012).In contrast, D-valine is non-toxic to plants but can be metabolized to a toxic product by a DAO transgene (Erikson et al. 2004;Gisby et al. 2012).In this study, we leveraged the dual functions of a DAO in metabolizing different D-amino acid substrates to develop a surrogate selection system for simplifying genome editing in Arabidopsis.This system can simultaneously facilitate the screening of CRISPR-edited alleles and subsequent elimination of mutant progeny containing the Cas9 transgene.

TvDAO confers D-serine resistance in Escherichia coli
To utilize a DAO-based surrogate selection marker for CRISPR editing, we first sought to screen a DAO with robust catalytic activity in detoxifying D-serine.For this purpose, we selected five homologous DAOs (Pilone 2000;Pollegioni et al. 2007Pollegioni et al. , 2018) ) from Rhodotorula gracilis (RgDAO), Homo sapiens (hDAO D31H), Fusarium verticillioides (FvDAO), Trigonopsis variabilis (TvDAO), and Mus musculus (MmDAO), respectively.These DAOs were codon-optimized (Supplementary Sequences) for optimal expression in Arabidopsis before gene synthesis.When using the Arabidopsis protoplast-based transient assay to evaluate the activity of these DAOs in detoxifying D-serine, we observed that Arabidopsis protoplasts were not sensitive to D-serine treatment.Considering that E. coli is a frequently used heterologous system for evaluating the function of a DAO in detoxifying D-amino acids (Pollegioni et al. 2007), we switched to E. coli to compare the activities of these DAOs.In three biological replicates, E. coli cells expressing TvDAO exhibited slightly better growth on the D-serine-containing medium than those expressing the other DAOs, particularly at a D-serine concentration of 60 mM (Fig. 1).Conversely, E. coli cells expressing the negative control CERK1, which encodes a receptor-like kinase from Arabidopsis, suffered obvious growth arrest in the presence of D-serine (Fig. 1).These findings suggest that all DAO transgenes are able to confer D-serine tolerance in E. coli.
TvDAO enables both positive and negative selection for transgenic Arabidopsis plants Considering that the TvDAO is not only smaller in size but also exhibited modestly stronger activity in conferring D-serine resistance in E. coli (Fig. 1), we next focused on TvDAO and attempted to validate whether it could be employed for D-serine-based positive selection and D-valine-based negative selection of transgenic plants.To this end, we generated transgenic Arabidopsis plants expressing TvDAO along with the HygR gene (Fig. 2A) for side-by-side comparison.When screening the transgenic T 1 plants on 1/2 Murashige and Skoog (MS) medium containing 5 mM D-serine or 25 mg L -1 hygromycin, the transgenic plants selected by D-serine exhibited a clearer resistance phenotype than those selected by hygromycin (Fig. 2B), implying that TvDAO may be a better selection marker gene than HygR in Arabidopsis.
The T 2 generation of two representative lines, TvDAO #2 and TvDAO #7, showed resistance segregation when germinated on the medium containing D-serine or Dvaline, whereas all wild-type plants were sensitive to D- serine but grew well on the D-valine-containing medium (Fig. 2C).Consistently, PCR-based genotyping of three randomly selected healthy seedlings (i.e., a1-a3) or dying seedlings (i.e., d1-d3) from the progeny of TvDAO #2 or TvDAO #7 grown in the presence of D-valine  showed that the a1-a3 were transgene-free, while the d1-d3 were transgenic (Fig. 2D).These results indicated that the TvDAO-based reporter supports both positive selection of transgenic plants by D-serine and negative selection of transgene-free plants by D-valine.
TvDAO-based selection system facilitates the screening of CRISPR-edited alleles in Arabidopsis Given that TvDAO can be used for both positive and negative selection in Arabidopsis, depending on the D- amino acid substrate, we hypothesized that it would serve as an excellent surrogate selection marker for enriching CRISPR-edited alleles and then eliminating Cas9-containing progeny.To achieve this, CRISPR-induced single-strand annealing (SSA) repair converting a dead TvDAO (dTvDAO) reporter to an active form was devised (Fig. 3A, and B).SSA is a homologous recombination-based DNA repair mechanism that promotes recombination between two repetitive DNA sequences flanking a double-stranded break (DSB), resulting in the deletion of the intervening fragment (Endo et al. 2021;Vu et al. 2022).To validate the effectiveness of SSA repair in plant cells, we tested a dead GFP (dGFP) reporter (Fig. S1A), in which GFP was split into two fragments sharing a 321-bp overlapping sequence (nucleotides 199-519).These two fragments were separated by a linker containing two consecutive stop codons and a human genome-derived Cas9 target site (TGATGA-Cas9 TS).To optimize the CRISPR-induced SSA efficiency, we compared three different human target sites (i.e., hEMX1-1, hEMX1-2 and hEfemp1) in the dGFP reporter using corresponding gRNAs, namely hEMX1-gRNA1, hEMX1-gRNA2 (Cong et al. 2013), and hEfemp1-gRNA (Xu et al. 2020a).None of these gRNAs have predictable off-target sites in Arabidopsis.Transfection of the dGFP reporters alone into Arabidopsis protoplasts failed to produce any GFP fluorescence, whereas coexpressing Cas9 and a gRNA targeting the cognate human target site efficiently restored GFP expression (Fig. S1B-D).Considering comparable GFP regeneration efficiencies between the three human target site/gRNA pairs, we chose the hEfemp1 TS/hEfemp1-gRNA pair to build the dTvDAO reporter because this pair has been used in planta in a recent study (Xu et al. 2020a).
We designed the dTvDAO reporter by duplicating nucleotides 280-788 of the TvDAO coding sequence and inserting the linker of TGATGA-hEfemp1 TS between the two duplicated sequences (i.e., LH and RH) (Fig. 3B).We constructed the binary plasmid dTvDAO-gRNAs-Cas9 [pHEE401E-UBQ10pro::dTvDAO-poly A-(tRNA-gRNA)s] to express Cas9 using the egg cell-specific EC1.2-EC1.1 fusion (EC1f) promoter (Wang et al. 2015) and to express dTvDAO and polycistronic tRNA-gRNA repeats as a single transcript unit using the UBQ10 promoter (Tang et al. 2019) (Fig. 3A).The polycistronic tRNA-gRNA repeats could produce multiple gRNAs through the endogenous tRNA-processing machinery (Xie et al. 2015).In addition to the hEfemp1-gRNA, we incorporated two additional gRNAs in the tRNA-gRNA repeats that target to three endogenous genes related to trichome development in Arabidopsis, namely ETC2, CPC, and TRY (Kirik et al. 2004).ETC2 was targeted by one gRNA (i.e., ETC2-gRNA), whereas CPC and TRY were targeted by the other gRNA (i.e., CPC/TRY-gRNA).Notably, the pHEE401E vector also encodes the HygR (Fig. 3A), allowing hygromycin selection of transgenic plants as an alternative option.As a negative control, we constructed the binary plasmid dTvDAO-Cas9 (pHEE401E-UBQ10pro::dTvDAO-poly A) without polycistronic tRNA-gRNA repeats.None of the transgenic T 1 We harvested a total of 3.3 mL seeds from the plants transformed using the Agrobacterium cells carrying the binary plasmid dTvDAO-gRNAs-Cas9.These seeds were divided into two fractions, one (* 3 mL seeds) for D- serine selection and the other (* 0.3 mL seeds) for hygromycin selection.Among a total of 28 D-serine-re- sistant plants obtained, 27 (that is, S1-S28 except S15) were identified by PCR-based genotyping as transgenic plants, which all generated TvDAO amplicons with an identical size as those from the TvDAO transgenic line #2 (Figs.3C and S2).This indicated successful recovery of TvDAO by SSA, which was further confirmed by Sanger sequencing of TvDAO amplicons from these plants (Fig. 3D).Among a total of 40 hygromycin-resistant plants obtained, 36 (that is, H1-H40 except H9, H14, H15 and H23) were identified as transgenic plants, which produced PCR amplicons of edited or non-edited dTvDAO (Figs.3C and S2).
All 27 D-serine-resistant transgenic T 1 plants harbored mutations for at least two of the three endogenous target genes (Fig. S3).Strikingly, 25 out of the 27 (92.6%)contained concurrent mutations across all three genes (Figs. 3D and S3).Consistently, slightly clustered trichomes could be seen on the leaf surface of some plants (Fig. 3E), as exemplified by Cas9 #S1 that harbored homozygous mutation in CPC and heterozygous mutations in ETC2 and TRY (Figs. 3D and S3).By contrast, only 19 out of the 36 (52.8%) hygromycinresistant transgenic T 1 plants contained concurrent mutations for all three target genes (Fig. S3).Notably, there were five plants (that is, H1, H27 and H33-35) containing no mutations at all for the four target loci including dTvDAO (Figs.S3 and S4).Importantly, D-ser- ine-resistant transgenic plants not only showed an exceedingly higher co-editing frequency but also higher editing frequencies at individual endogenous target sites than hygromycin-resistant transgenic plants, namely 100% versus 66.7% for CPC, 96.3% versus 61.1% for ETC2, and 96.3% versus 77.8% for TRY (Fig. 3F).These findings demonstrated that the dTvDAO surrogate selection marker can ease the screening of mutant alleles produced by multiplex CRISPR editing in Arabidopsis.
TvDAO-based selection system facilitates the identification of Cas9-free progeny Next, we evaluated whether the TvDAO-based selection system could also be used for identifying Cas9-free mutant progeny.To this end, we germinated the T 2 progeny of the Cas9 #S1 plant on the 1/2 MS medium, with or without 15 mM D-valine.As anticipated, some seedlings were sensitive to D-valine (Fig. 4A).PCR-based genotyping revealed that three randomly selected healthy seedlings (i.e., A1-A3) were all Cas9-and TvDAOfree, whereas three randomly selected dying seedlings (i.e., D1-D3) all contained the transgenes (Fig. 4B).Sanger sequencing of target amplicons validated stable inheritance of T 1 mutations at all three endogenous target sites in the T 2 plant Cas9 #S1-A3, in which the mutations at the ETC2 and TRY loci became homozygous (Fig. 4C).Therefore, the Cas9 #S1-A3 plant represented a Cas9-free cpc etc2 try triple mutant allele, which displayed highly clustered trichomes (Fig. 4D) as previously reported (Kirik et al. 2004).These results suggested that D-valine-based negative selection allows efficient identification of Cas9-free mutant alleles.

DISCUSSION
Screening Cas9-induced mutant alleles from herbicideor antibiotic-resistant transgenic plants and isolating Cas9-free mutant progeny are two labor-consuming processes during plant genome editing.Although several surrogate selection systems have been explored to facilitate either process (Liu et al. 2019;Wang and Chen 2020;Xu et al. 2020aXu et al. , 2020b;;Kong et al. 2021;Rinne et al. 2021;Lao et al. 2023;Tian et al. 2023), to our knowledge, no selection marker can work for both processes.In this work, we demonstrate an innovative approach of using a TvDAO-based reporter as a single surrogate selection marker to ease the two demanding processes in Arabidopsis, particularly during multiplex CRISPR editing.Our streamlined approach involves five steps (Fig. 5): (1) construction of the dTvDAO reportercontaining binary plasmid co-expressing Cas9/gRNAs, in which multiple gRNAs are assembled as polycistronic tRNA-gRNA repeats; (2) Agrobacterium-mediated transformation of Arabidopsis; (3) selection of T 1 generation using 5 mM D-serine; (4) Sanger sequencing of target amplicons to validate multigene editing in survival T 1 alleles; (5) selection of T 2 generation using 15 mM D-valine to obtain survival Cas9-free mutants.
In this study, we leveraged the CRISPR-induced SSA repair for seamless restoration of a reporter gene from its inactive version.Indeed, this strategy worked as expected in both Arabidopsis protoplasts and transgenic plants, for both GFP and TvDAO (Figs. 3 and S1).Notably, when we combined the CRISPR-induced SSA repair of the non-functional dTvDAO with D-serine-conditioned positive selection, we could observe significantly increased multiplex editing at the three endogenous   5 Overview of TvDAO-facilitated screening for CRISPR-edited and Cas9-free Arabidopsis mutant alleles.The gRNAs targeting the human hEfemp1 site in dTvDAO (gRNA 1 ), or endogenous genes of interest (gRNA 2 to gRNA n ), are expressed as polycistronic tRNA-gRNA repeats, which are assembled by Golden Gate assembly and inserted into the pHEE401E vector, via the rare-cut restriction site I-CeuI.This allows gRNAs to be produced along with the dTvDAO reporter as a single transcript unit driven by the strong constitutive UBQ10 promoter.Cas9 is expressed under the egg cell-specific EC1f promoter.By Agrobacterium-mediated transformation, transgenic Arabidopsis plants expressing dTvDAO, gRNAs, and Cas9 are generated.T 0 seeds are selected by 5 mM D-serine to enrich CRISPR-edited T 1 alleles (green alive seedlings), which are further validated by target amplicon Sanger sequencing.Subsequently, their Cas9-free T 2 alleles (green healthy seedlings) are selected by 15 mM D-valine  target sites when compared to the routinely used hygromycin selection (Figs.3F and S3), suggesting that multiple co-expressed gRNAs can mediate CRISPR editing with a considerable level of co-efficiency.Moreover, we noted an obvious trend that D-serine selection greatly stimulated TvDAO editing, as 24 out of the 27 (88.9%)D-serine-resistant T 1 plants carried homozygous TvDAO mutations (Fig. S3).In contrast, only 2 out of the 36 (5.6%) hygromycin-resistant T 1 plants contained homozygous TvDAO mutations (Fig. S3).It is very likely that the selection pressure, per se, also promotes the activity of CRISPR/Cas9 to produce functional TvDAO for plant survival.These combinatorial effects thus allow our surrogate selection system to effectively enrich mutant alleles with multigene editing.
It has not escaped our attention that, when transformed with the same binary construct conferring both D-serine and hygromycin resistance, transgenic plants selected by D-serine exhibited a reduced positive rate relative to those selected by hygromycin (Fig. 3E).A reasonable explanation for this observation is that the SSA is only a minor repair mechanism for DNA DSBs in higher eukaryotes (Stinson and Loparo 2021;Oh and Myung 2022), thus being outcompeted by the dominant non-homologous end joining (NHEJ) repair system (Fiorenza et al. 2001).Therefore, some transgenic plants with a Cas9-induced DSB in the dTvDAO reporter failed to generate an active TvDAO, via the SSA repair, and were killed by D-serine selection.The low efficiency of SSA repair in regenerating TvDAO is thus a bottleneck in our current strategy.One way for future optimization could be to use the NHEJ-based traffic light reporter (de Jong et al. 2020) of dTvDAO to replace the SSA-based dTvDAO reporter.In the so-called traffic light reporter, a spacer would be inserted after the start codon of TvDAO, which contains a Cas9 target sequence (such as the hEfemp1 TS) and a downstream stop codon that is in frame with the start codon.When Cas9 induces a DSB at the spacer, the NHEJ-mediated frameshift of either ? 1 nt or ? 2 nt in this spacer would dismiss the stop codon and restore TvDAO production.
In summary, our TvDAO-based selection system allows both D-serine-based positive selection to facilitate CRISPR mutant screening and subsequent D-valine- based negative selection to remove Cas9-containing mutant offspring, thus significantly improving the time and labor efficiencies in isolating Cas9-free multigene mutant alleles in Arabidopsis.Given that D-serine is toxic for a wide range of plant species including tobacco, tomato, spruce, poplar, maize, and barley (Erikson et al. 2004) and that the DAO-induced D-serine resistance has also been validated in tobacco (Lim et al. 2007;Gisby et al. 2012), it is possible to transfer our TvDAO-based selection system to other plant species to simplify multiplex CRISPR editing.Meanwhile, a modified dTvDAO selection system should be useful in promoting base editing or prime editing in plants.

Plant materials and growth conditions
The Arabidopsis thaliana ecotype Col-0 was used as the wild-type plants in this study.The transgenic T 1 plants were screened on 1/2 9 MS medium containing 5 mM D-serine or 25 mg L -1 hygromycin.The T 2 plants were screened on 1/2 MS medium containing 15 mM D-va- line.After stratification at 4 °C for 2 days, the seeds were grown in a plant growth chamber (Ningbo Saifu, China) under a photoperiod of 12 h light (75 lmol m -2 s -1 ) at 23 °C and 12 h dark at 21 °C.The resistant seedlings were transferred to Jiffy soil (Jiffy Group, Netherlands) and grown in a plant growth room under a photoperiod of 12 h light at 23 °C and 12 h dark at 21 °C, with humidity maintained at 65%.

Plasmid construction
The coding sequences of RgDAO, hDAO D31H, FvDAO, TvDAO, and MmDAO were codon-optimized according to the codon bias of Arabidopsis thaliana (Supplemental sequences).The sequences were then synthesized and inserted into the BamHI and StuI sites of the HBT-35SPPDK-2 9 FLAG vector by GENCEFE Biotech Company (Wuxi, China).For CRISPR-induced SSA repair of dGFP, dGFP containing two overlapping fragments (nucleotides 199-519) of GFP and TGATGA-Cas9 TS was cloned by overlap PCR, then inserted into the BamHI and StuI sites of the HBT-35SPPDK-2 9 HA vector, resulting in the HBT-35SPPDK-dGFP(TS)-2 9 HA plasmid.The AtU6-26pro::gRNA expression cassettes were assembled by oligo phosphorylation and annealing, and then inserted into the BsaI site of the pUC119 vector.

D-serine tolerance assay in E. coli
The E. coli cells expressing RgDAO, hDAO D31H, FvDAO, TvDAO, MmDAO, or CERK1 were cultured at 37 °C overnight.The cultures were then diluted to an OD 600 of 0.5, 0.1, 0.02, and 0.005 before being applied onto LB medium containing 50 mg L -1 ampicillin, with or without 50 mM or 60 mM D-serine.The plates were incubated at 37 °C for approximately 20 h, and the tolerance phenotypes were recorded using a camera.

Protoplast isolation and transfection
Arabidopsis protoplasts were extracted and transfected as previously described, using the leaves of 4-week-old plants (Yoo et al. 2007).The dGFP reporter was transiently expressed alone or co-expressed with Cas9 and a cognate gRNA to the human target site in the dGFP reporter in Arabidopsis protoplasts.After overnight culture, the fluorescence was observed using a Leica DM8i C inverted microscope (Leica Biosystems, Germany).

Generation of transgenic plants
The binary plasmids TvDAO, dTvDAO-Cas9, or dTvDAO-gRNAs-Cas9 were introduced into Agrobacterium tumefaciens strain GV3101 by electroporation.The Agrobacterium cells carrying the appropriate binary plasmids were used to transform Arabidopsis using the widely used floral dip method (Clough and Bent 1998).

Genomic DNA extraction
Crude genomic DNA (gDNA) extracts of Arabidopsis plants were obtained by homogenizing three leaves from a single plant in TKE buffer (100 mM Tris-HCl, pH 9.5, 1 M KCl, 10 mM EDTA), followed by incubation at 70 °C for 30 min.The tenfold diluted gDNA extract was used as a PCR template for genotyping.

Genotyping of Arabidopsis plants
The Green Taq Mix (Vazyme, China) was used for genotyping.The PCR amplicons were separated by agarose gel electrophoresis.The gel strips containing PCR amplicons were excised and used for Sanger sequencing to validate the sequences.The primers used for genotyping PCR in this work are listed in Supplementary Table S1.

Fig. 1
Fig. 1 D-amino acid oxidase confers D-serine tolerance in Escherichia coli.E. coli cells expressing Rhodotorula gracilis d-amino acid oxidase (RgDAO), Homo sapiens DAO (hDAO D31H), Fusarium verticillioides DAO (FvDAO), Trigonopsis variabilis DAO (TvDAO), or Mus musculus DAO (MmDAO) were diluted to indicated cell concentrations (OD 600 ) and inoculated on the growth medium containing 0, 50 or 60 mM D-serine.E. coli cells expressing Arabidopsis thaliana CERK1 encoding a receptor kinase were used as a negative control.TvDAO used for further study is highlighted in red.These experiments were repeated three times with similar results

Fig. 2
Fig. 2 TvDAO enables both positive and negative selection of transgenic Arabidopsis plants.A Diagram of the expression cassettes for TvDAO and hygromycin resistance gene (HygR) in the binary plasmid.LB, T-DNA left border; RB, T-DNA right border; HSP ter , Heat shock protein terminator.B Transgenic TvDAO plant selected by D-serine shows a clearer resistance phenotype than that selected by hygromycin.Seedlings indicated by white arrows are transgenic plants.Scale bar = 1 cm.C Progeny of D-serine-resistant lines, TvDAO #2 and TvDAO #7, show resistance segregation in both D-serine-based positive selection and D-valine-based negative selection.Scale bar = 1 cm.D PCR-based genotyping reveals that D-valine resistance or susceptibility is consistent with the absence or presence of TvDAO transgene.a1-a3 and d1-d3 are randomly selected alive or dying seedlings, respectively, on the D-valine-containing medium.ACTIN2 was used as an internal control

Fig. 4
Fig. 4 TvDAO can be used to eliminate Cas9-carrying mutant progeny.A Progeny of the transgenic Cas9 #S1 plant exhibit resistance segregation on 1/2 MS medium containing 15 mM D-valine.Scale bar = 1 cm.B PCR-based genotyping confirms transgene elimination in survival T 2 plants of Cas9 #S1 under D-valine-conditioned negative selection.A1-A3 and D1-D3 are randomly selected alive or dying seedlings, respectively.C Sanger sequencing of target amplicons validates inherited mutations at the three endogenous target sites in the T 2 plant Cas9 #S1-A3.Black bold letters mark PAMs and target sequences of gRNAs are underlined.Insertion and deletions are in red.D The Cas9 #S1-A3 plant displays highly clustered trichomes as indicated by red arrows.Scale bar = 1 cm

Fig.
Fig.5Overview of TvDAO-facilitated screening for CRISPR-edited and Cas9-free Arabidopsis mutant alleles.The gRNAs targeting the human hEfemp1 site in dTvDAO (gRNA 1 ), or endogenous genes of interest (gRNA 2 to gRNA n ), are expressed as polycistronic tRNA-gRNA repeats, which are assembled by Golden Gate assembly and inserted into the pHEE401E vector, via the rare-cut restriction site I-CeuI.This allows gRNAs to be produced along with the dTvDAO reporter as a single transcript unit driven by the strong constitutive UBQ10 promoter.Cas9 is expressed under the egg cell-specific EC1f promoter.By Agrobacterium-mediated transformation, transgenic Arabidopsis plants expressing dTvDAO, gRNAs, and Cas9 are generated.T 0 seeds are selected by 5 mM D-serine to enrich CRISPR-edited T 1 alleles (green alive seedlings), which are further validated by target amplicon Sanger sequencing.Subsequently, their Cas9-free T 2 alleles (green healthy seedlings) are selected by 15 mMD-valine