Effects of post-mining forest restoration and alternative land uses on ground-dwelling arthropods in Ghana

In an Afrotropical region experiencing massive deforestation, restoration approaches should provide sustainable solutions for recovering biodiversity. Arthropods are a sensitive taxonomic group for habitat alteration by deforestation and can be good indicators for restoration studies. Ground-dwelling arthropods provide important ecosystem functions, such as predation or organic matter decomposition, thereby contributing to ecosystem functionality. The consequences of post-mining management on arthropods in the Afrotropical region remain understudied. We carried out a comprehensive sampling of ground-dwelling arthropods in the dry and wet seasons across four land-use types in the semi-deciduous forest zone of Ghana. We then analysed whether the specific tree communities, vegetation structure and seasonal differences affected arthropod communities in the restored post-mining forest compared to the dominant alternative land-use type (agroforestry plantation), a natural reference (natural forest) or an unmanaged former mining area (gravel mine). In total, 43,364 arthropods were sampled and assigned to 78 taxonomic groups representing 14 order/sub-order, 28 beetle families, 25 spider families, 5 hunting guilds of spiders and 6 trophic groups of beetles. Overall, Araneae, Coleoptera, Hymenoptera and Orthoptera all had higher activity densities in the wet season. The vegetation structure of the three land-use types with trees supported a greater overall activity density of arthropods and a more diverse functional composition compared to the unmanaged gravel site. Pronounced variation between the dry and wet seasons further influenced the taxonomic and functional composition. The active forest restoration of this post-mining area is a promising approach to drive arthropod communities towards a comparable state observed in the natural forest.


Introduction
Arthropods play a key role in ecosystem functioning and provide important ecosystem services for human societies, including local communities in Western Africa (Culliney, 2013;Høye & Culler, 2018;Isaacs et al., 2009;Sagi & Hawlena, 2021). Between 5 and 10 million terrestrial arthropod species have been described worldwide (Novotny et al., 2002;Ødegaard, 2000;Stork, 2018), of which up to 3.7 million species are found in the tropics (May, 2010). The Afrotropical region features a wide range of natural habitats with diverse plant and animal communities (Stuart et al., 1990), many of which are endemic to the region. In terms of ecosystem services provided by arthropods (Biondi et al., 2015;Dangles & Casas, 2019), pollination, nutrient regulation, soil formation and pest control contribute to human well-being (Birkhofer et al., 2018;Rader et al., 2016;Schowalter, 2017). Thereby, arthropods contribute to global food security (van der Sluijs & Vaage, 2016) and consequently reduce poverty (Dangles & Casas, 2019), as for example, predators (e.g. spiders) prey on herbivorous organisms, which could become pests on crops (Nyffeler et al., 2016).
Arthropod populations and diversity are threatened by human activities stemming from agricultural intensification, mining and land-use conversion or habitat loss Damptey et al., 2022a;Picanço et al., 2017;Seibold et al., 2019). Mining has been a crucial economic sector in many developing countries but comes with costs for biodiversity and interrupts the provision of several ecosystem services to human society (Asare et al., 2022;Ofosu et al., 2020;Schueler et al., 2011;Sonter et al., 2018). For instance, surface mining leads to the degradation of forests that would have otherwise provided habitat for pollinating or seed-dispersing arthropods, with consequences for local and global food security (Sonter et al., 2018). In addition, a shortage of productive land coupled with changing local and regional weather conditions because of land degradation and climate change leads to a decline in farm productivity and an increase in food insecurity globally (Ime & Ekong, 2015). Similarly, the removal of trees during mining eliminates the ability of forests to store carbon, with severe implications for climate change (Ontl et al., 2020). Moreover, a land-use change that involves the conversion of a particular land-use type (e.g. natural forest) to alternative land use (e.g. agroforestry plantations) is known to result in a loss of arthropod biodiversity in some regions (e.g. Newbold, 2018). The high rate of conversion of tropical forests to other land uses is also anticipated to have consequences for both local and regional biodiversity, with cascading effects on other ecological processes (Schroeder et al., 2021). For instance, it is very obvious that the conversion of forests to agricultural lands or recreational parks affects arthropod diversity on a global scale (Millard et al., 2021;Perry et al., 2016), but the effect of restoring post-mining areas through restoration or agroforestry on arthropods is relatively unknown in Ghana and responses may differ among different taxonomic or functional arthropod groups. Restoration in Ghana sometimes takes the form of agroforestry, where economic and ecological valued tree species are interplanted with food crops to meet societal needs or the form of enrichment planting to restore degraded forests (Damptey et al., 2021). Although several studies have discussed the devastating effects of land-use change on arthropods elsewhere in the world (e.g. Cardoso et al., 2020;Gagnarli et al., 2021), the consequences of land-use conversion and post-mining restoration in Ghana and Western Africa, in general, remain understudied.
In addition to the impact of land-use changes, pronounced seasonal differences will also affect the taxonomic and functional composition of arthropod communities (Wardhaugh et al., 2018). In Ghana, the two major seasons are based on the amount of precipitation, differences in temperature and the number of dry months (Owusu & Waylen, 2012). The characteristic rainfall in the wet season (April to July) should facilitate the emergence of arthropods from soil and the development of large patches of potential host plants (Basset et al., 2015). However, the dry season, with its long period of drought conditions, is accompanied by water stress-inducing physiological constraints and limited resource availability, thereby limiting the ability of arthropods to perform essential ecological functions and other services (Huberty & Denno, 2004).
To address the question of how post-mining restoration affects arthropod communities compared to a natural reference system, an alternative land-use type and an unmanaged former mining area in the two major seasons in Ghana, we tested the following hypotheses: (i) land-use types with diverse tree communities and heterogeneous vegetation structure (natural and restored forest) support a higher number of arthropod orders, functional groups and overall activity density than in agroforestry plantation and former mining area and (ii) the effect of land-use types on the taxonomic and functional composition of arthropods depends on the season with the strongest expected differences between landuse types with trees and agroforestry plantation and former mining area in the wet season.

Study area
The studied land-use types include the following: (1) an actively "restored forest" as restoration activity (Terchire restoration area; RF), (2) an "agroforestry plantation" as an alternative land-use (Bosomkese forest reserve; AF), (3) a "natural forest" as a natural reference (Asukese forest reserve; NF) and (4) an unmanaged "gravel site" as an unmanaged system (Terchire abandoned gravel mine site; GS). All land-use types lie in a semi-deciduous forest zone (SDFZ) and are located in the Ahafo and Bono regions of Ghana ( Fig. 1; Damptey et al., 2022b). The forest zone is characterised by a mean daily temperature of 20 °C and annual precipitation ranging between 900 and 1500 mm (rainfall peaks between July and August; Damptey et al., 2021).

Sampling design
The four land-use types were studied across both seasons (dry and wet) in the semi-deciduous forest zone (SDFZ) of Ghana. Each land use was studied in eight replicate 20 × 20 m plots, resulting in 32 study plots. Basic vegetation attributes (Table 1) were surveyed and used to describe the major dendrological characteristics of each land-use type (see also Damptey et al., 2020Damptey et al., , 2021. A standardised trapping method involving the use of pitfall traps was used to sample and estimate the activity density (A_D: number of samples caught divided by the sampling effort) of ground-dwelling arthropods based on their locomotory activities (Greenslade, 1964;Perner & Schueler, 2004).
Ground-dwelling arthropod communities were continuously sampled, with five pitfall traps in each plot being emptied weekly for 10 weeks in each sampling season. The first campaign was conducted in the dry season (January to March 2019), followed by the wet season campaign (June to August 2019). Pitfall traps were filled with a 50:50% mixture of propylene glycol and water, and all pitfall traps were covered by small roofs to avoid dilution of the trap liquid by rain (Underwood & Quinn, 2010). Pitfall trap samples were stored in 70% ethanol and later sorted into taxonomic groups (order, suborder or family) according to available identification keys (for spiders; Dippenaar-Schoeman & Jocqué,

Data analyses
Arthropod community data for plots within each land-use type were pooled together and log-transformed [log (x + 1)]. Activity density (A_D) of arthropods for land-use types and seasons was estimated based on the number of individuals sampled divided by the sampling effort (Greenslade, 1964). A non-metric multidimensional scaling ordination (NMDS) based on Bray-Curtis similarities was created to visually represent the multivariate relationship within and between sampling plots of different land-use types and seasons. The goodness of fit of NMDS ordinations was evaluated using the 2-d stress value (Clarke et al., 2014). For the NMDS based on the taxonomic composition of all arthropods, vectors were superimposed for orders with Pearson correlation coefficients > 0.2 with axis scores. For the identification of Coleoptera and Araneae families and functional groups that were characteristic of land-use types or seasons, similarity percentage analysis (SIMPER) was used based on Bray-Curtis similarity and a cut-off value of 70% for the total contribution (Somerfield & Clarke, 2013

Arthropod taxonomic composition
In total, 43,364 arthropods were sampled and assigned to 78 taxonomic groups representing 14 order/sub-order ("Appendix 1"), 28 beetle families (divided into 6 trophic groups) and 25 spider families (divided into 5 hunting guilds). The arthropod communities at the former gravel mine are unique for both seasons, followed by a gradient from the agroforestry plantation to the restored and the natural forest communities with increasing activity densities of Blattodea, Julida, Hymenoptera, Coleoptera and Araneae along that gradient independent of the season (Fig. 2). The restored forest plots have an intermediate position between the agroforestry plantation and the natural forest arthropod communities. Within land-use types, Hemiptera had a higher activity density in the dry season compared to the wet season. Blattodea and Julida had the highest activity density in the natural forest and were absent from the gravel site. Within land-use types, Orthoptera had a higher activity density in the wet season, and Polydesmida were only present in the wet season.

Spider family composition
The total activity density of spiders was higher in NF (3.03) than RF (2.79), AF (1.59) and GS (1.35) and also higher in the wet (5.44) than in the dry season (3.32). The families Lycosidae, Salticidae and Zodariidae, dominated communities, amounting to more than 50% of all individuals in each of the four land-use types. Most families (e.g. Corinnidae, Ctenidae, Migidae, Zodariidae) had higher activity densities in the wet season, except Oxyopidae, which had a higher activity density in the dry season, and Lycosidae and Salticidae, which did not differ much between seasons (Fig. 3). Spider family composition showed a gradient from the dry season agroforestry plantation and the gravel site for both seasons towards the restored forest and the natural forest plots for both seasons. For spider communities, the wet season agroforestry plots hold an intermediate position between the restored and natural forest plots.
The average dissimilarity between the dry and wet season plots was 44% and was driven by a higher activity density of Salticidae, Zodariidae, Ctenidae, Corinnidae, Lycosidae and Cyrtaucheniidae in the wet season (Table 2). In terms of dominance, the dry season plots were dominated by Lycosidae (28% of all individuals), Zodariidae (25%), Salticidae (15%) and Corinnidae (12%) and differed from wet season plots due to an even higher dominance of Lycosidae (52%) but lower dominance of Zodariidae (16%) and Salticidae (12%) in the wet season.

Spider hunting guilds
Ground hunters (A_D = 4.13) were the most dominant group across land-use types, followed by other hunters (A_D = 2.61), specialists (A_D = 1.59), sensing web (A_D = 0.35) and ambush hunters (A_D = 0.07). Ground hunters were more active in RF than GS, AF and NF (Fig. 4A). Sensing spiders were rather active in the AF than NF, RF and GS (Fig. 4B). Other hunters were also more active in NF than RF, AF and GS (Fig. 4C). For specialist's spiders, higher activity density was recorded for NF compared to RF, GS and AF (Fig. 4D). Non-metric multidimensional scaling ordination based on log-transformed (log(x + 1)) activity densities of beetles and Bray-Curtis similarities between plots of different land-use types (○ Restored forest, × Agroforestry plantation, + Natural forest and ◊ Gravel site) and seasons (green = wet, red = dry). The 2-d stress value is 0.13. Symbols of each land-use type and season combination are connected by minimum spanning trees

Beetle family composition
The taxonomic composition of beetle communities differed significantly between land-use types (F 3,27 = 14.52; p < 0.001) and seasons (F 1,27 = 46.14; p < 0.001). The differences between land-use types did depend on the season (F 3,27 = 10.80; p < 0.001). Pairwise statistical comparisons indicated significant differences in family composition between beetle communities of the natural forest and the agroforestry plantation (t = 4.41, p < 0.001), restored forest (t = 3.24, p < 0.001), gravel site (t = 4.50, p < 0.001) and between the restored forest and the agroforestry plantation (t = 2.02, p = 0.005), gravel site (t = 3.86, p < 0.001) as well as between the agroforestry plantation and the gravel site (t = 3.78, p < 0.001). Beetle communities at the former gravel mine were unique and more heterogeneous during the dry season than during the wet season, with a higher activity density of Elateridae at the gravel site plots (Fig. 5). The beetle communities in the wet season restored and natural forest plots resembled each other and were characterised by higher activity densities of Histeridae, Hydrophilidae and Staphylinidae. While beetle communities did not differ between the natural and restored forests in the wet season, they differed in the dry season. Beetle communities in the agroforestry plantation in the wet season resembled forest communities in the dry season more than other communities in habitats with trees in the wet season.
In terms of dominance, the dry season plots were dominated by Tenebrionidae (36% of all individuals), Nitidulidae (20%), Carabidae (10%) and Erotylidae (8%) and differed from wet season plots by and even higher dominance by Scarabaeidae (26%) and Carabidae (22%) but lower dominance of Staphylinidae (13%), Nitidulidae (9%) and Cetonidae (7%). The average dissimilarity between the dry and wet season plots was 65% and was driven by a higher activity density of Scarabaeidae, Carabidae, Staphylinidae, Hydrophilidae, Histeridae and Cetonidae in the wet season but a higher activity density of Tenebrionidae and Nitidulidae in the dry season (Table 3).

Discussion
Our comparison of arthropod communities between an actively restored post-mining forest, a dominant alternative land-use type (agroforestry plantation), a natural reference (natural forest) and an unmanaged former mining area (gravel mine) provides the first assessment of the effects of land-use decisions in former mining areas in Western Africa on arthropod communities. The observed pronounced differences between communities at a relatively coarse level of taxonomic (order to family) and functional (spider hunting guilds and beetle trophic groups) classification emphasise the need to address these effects in times of global insect decline (Cardoso et al., 2020;Wagner et al., 2021).

Taxonomic composition of arthropod communities
The observed dominance structure in the studied arthropod communities supports our hypothesis that land-use types with diverse and heterogeneous vegetation structure (Tab. 1) support a greater range of arthropod taxa and overall higher activity density (see also Gardner et al., 1995;Mata et al., 2021;Damptey et al., 2022a). The land-use types dominated by trees in this study (natural forest, agroforestry plantation and restored forest) offered additional niches and resources to support the activity of arthropods compared to the unrestored gravel mine. Meloni et al. (2020) showed that even ground-dwelling arthropods benefit from more diverse vegetation and the resulting habitat attributes. Diverse vegetation provides more refuge and protection from predators, resulting in higher survival and reproductive success in potential prey taxa (Wenninger & Inouye, 2008;Zou et al., 2013). For predators, diverse vegetation often correlates with higher prey availability supplying food needs (Schuldt et al., 2011;Staab & Schuldt, 2020;Štokmane & Spuņģis, 2016). Deadwood and litter further promote the activity of detritivores, fungivores and arthropod predators that are part of the detritivore food web in forests (Sereda et al., 2012(Sereda et al., , 2015Tonin et al., 2018). Heimonen et al. (2013) emphasised the pronounced seasonal variation of herbivorous insects (e.g. mostly Orthopteroidea) that is common in tropical rain forests. For example, increasing resource concentration in the wet season is a significant factor in determining the population size in specialist herbivore populations (Doublet et al., 2019) and beetle communities (de Castro-Arrazola et al., 2018). The observed differences between the two seasons support our hypothesis that arthropod taxonomic composition is strongly influenced by seasonality across the different land-use types in our study (Lingbeek et al., 2017). Richards and Windsor (2007) observed significant seasonal variation in arthropod abundance in a lowland moist forest. Similarly, Wagner (2001) observed significant seasonal changes in arthropod fauna in a rain forest. Our study observed a higher activity density of Orthoptera (mainly herbivorous) and Polydesmida (mainly detritivorous) in the wet season than in the dry season. Several factors related to macro-and micro-climatic changes (e.g. temperature, rainfall, humidity, day length, decomposition rate of organic materials etc.) might have caused this pattern (Halsch et al., 2021;Wardhaugh et al., 2018;Belchior et al., 2016;Anu et al., 2009). In addition to abiotic conditions, food resources fluctuate seasonally, further affecting arthropod emergence, activity and reproduction (Richards & Windsor, 2007;Silva et al., 2011). Therefore, both structure-and resource-mediated effects likely affected arthropod communities between the seasons (Diehl et al. 2013). Independent of season, arthropod communities changed along a management intensity gradient in the sequence of agroforestry plantations to actively restore to natural forest arthropod communities. Therefore, the restored communities hold an intermediate position between the plantations and natural forests.

Spider families and hunting guilds
Similar to arthropod communities in general, spider communities are affected by vegetation structure, the presence of potential prey, as well as changes in abiotic conditions (Müller et al., 2022;Rosa et al., 2018;Yamazaki et al., 2017). The restored and natural forests were taxonomically richer than the agroforestry plantation and the gravel site, reflecting the various ecological niches that forest ecosystems provide to arthropods (Rosa et al., 2018). Generally, more complex vegetation offers a wider range of prey (e.g. (Diehl et al., 2013) as well as more diverse niches for spiders (Cardoso et al., 2011;Stańska et al., 2018). The observed higher activity density of spiders in the wet season results from precipitation, which drives plant growth as food for insects acting as prey for spiders (Rodríguez-Rodríguez et al., 2015). Spiders that construct sensing webs had higher activity densities in the land-use types dominated by trees resulting from the higher vegetation density and availability of web sites (see Pinto et al., 2021). Balfour and Rypstra (1998) emphasised the role of habitat structure for web support and the availability of suitable microhabitats for web-building spiders. Ambush hunters also had a higher activity density in land-use types that were dominated by trees. Ambush hunters (e.g. Thomisidae) often hide in flowers or on leaves to catch prey (Heiling et al., 2006;Willemart & Lacava, 2017) and, therefore, also rely on vegetation structure.

Beetle families and trophic groups
The activity density of beetles even differed between the three land-use types with trees, with the restored forest (dominated by non-native tree species, e.g. Tectona grandis, Senna siamia) recording lower activity densities than the agroforestry plantation and the natural forest. This trend is in line with previous studies that observed a lower beetle diversity in a non-native forest plantation, such as a restored forest, compared to an old-native forest (Fischer & Lindenmayer, 2007). This pattern could be attributed to the fact that younger restored forests still support fewer tree species with limited ability to offer food and niches compared to forests of intermediate age (Lachat et al., 2012).
Moreover, the lower activity density of beetles in the open gravel site could be attributed to the absence or limited availability of suitable habitats and food resources (Perry et al., 2016). The family Cetoniidae associated with the land-use types with trees has feeding preferences for plant tissues, exudates and organic materials (deadwood) (Mudge et al., 2012), which characterised the forest plots in this study. The higher activity density of Cetoniidae in the tree land-use types could be due to the potential existence of numerous ant colonies (not quantified in this study) for which several species of Cetonidae are predators (Holm & Marais, 1992). Expectedly, most beetle families showed higher activity density in the wet than in the dry season, in line with previous studies documenting higher diversities of beetles compared to the dry season (Andresen, 1999;Nyeko, 2009) and often attributed to the higher quality and quantity of food resources in the wet season (Wardhaugh et al., 2018).
Similar to the activity density of arthropods, all trophic groups of beetles classified in this study showed significantly higher activity density in the "tree" land-use types than in the "open" gravel site affirming the positive relationship between beetle functional groups and high vegetation structure (Damptey et al., 2022a;Sattler et al., 2010). Trophic groups such as detritivores (Mestre et al., 2018;Parisi et al., 2018;Wende et al., 2017) or herbivores (O'Brien et al., 2017) may have benefited from resources and habitat conditions provided by deadwood and leaf litter in the land-use types with trees. Similar to spiders, predaceous beetles may have also benefited from the higher prey availability in these land-use types (Damptey et al., 2021;Diehl et al., 2013).

Conclusion
The studied active forest restoration shows some promise in moving arthropod communities towards states observed in the natural forest, but arthropod communities in the agroforestry plantations were already more dissimilar. The patterns in arthropod communities observed for the land-use types depended on seasons, with the wet season making essential resources available for arthropods. Leaving former mining sites unmanaged is not a promising option, as arthropod communities and their habitat resource requirements were poor in the gravel site compared to the restored and natural reference forest and even the agroforestry plantation. We recommend that restoration activities in degraded post-mining regions of Ghana should focus on using mostly native tree species since they have the ability to supply habitat and food resources tailored to the needs of local biodiversity.

Acknowledgements
We are grateful to Newmont Gold Ghana Limited, the Forest Service Division of Ghana and the Terchire community for making the study sites available. We declare that no financial support was received from Newmont Gold Ghana Limited or the Forest Service Division of Ghana. We would like to thank the reviewers and the editor for their comments on a previous version, and we appreciate the contributions of Daniel Kwame Debrah and Pascal Agro Prince during the fieldwork. Kwadwo Gyasi Asante, Lanya Feng, Benjamin Schnerch, Sarina Böttner, Julia Yeboah, Tarek Hossain Raju and Christos Konstantinos Paxinos helped with sorting the arthropods into various taxonomic groups. We also appreciate the logistical support and guidance of Dr. Danilo Harms. The scholarship support from the Catholic Academic Exchange Service (KAAD) to FGD is greatly appreciated.
Author contributions All authors contributed to the study's conception and design. Material preparation, data collection and analysis were performed by FGD, ED and KB. FGD wrote the first draft of the manuscript, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Funding Open Access funding enabled and organized by Projekt DEAL.

Data availability
The data that support the findings of this study are openly available in figshare https:// doi. org/ 10. 6084/ m9. figsh are. 16556 211. v1

Conflict of interest
On behalf of all authors, the corresponding author states no conflict of interest.
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