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Genetic characterization of duck hepatitis B viruses from Anhui Province, China

  • Veterinary Microbiology - Short Communication
  • Published:
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Abstract

Duck hepatitis B virus (DHBV) infection model was frequently used as the experimental model for human hepatitis B virus (HBV) research. In order to decipher the genetic characteristics of DHBVs from Anhui province of China, 120 duck liver tissue samples were collected and subjected to PCR screening, and 28 samples were detected as DHBV positive. Subsequently, five DHBV-positive samples were selected for genome-wide amplification and a comprehensive analysis. Comparative analysis of complete genome sequences using the MegAlign program showed that five strains of DHBVs shared 94.5–96.3% with each other and 93.2–98.7% with other reference strains in GenBank. The phylogenetic analysis showed that all five DHBV strains belonged to the evolutionary branch of “Chinese DHBV” isolates or DHBV-2. Importantly, three potential intra-genotypic recombination events, between strains AAU-6 and Guilin, strains AAU-1 and GD3, and strains AAU-6 and AAU-1, were respectively found using the RDP and SimPlot softwares and considered the first report in avihepadnaviruses. These results not only improve our understanding for molecular prevalence status of DHBV among ducks, but also provide a reference for recombination mechanism of HBV.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This study was supported by the National Natural Science Foundation of China (Nos. 32000109, 32272982), Shanghai Sailing Program (20YF1457700), Natural Science Foundation of Shanghai (22ZR1476300), and Shanghai Municipal Science and Technology Major Project (ZD2021CY001).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China

    Yumeng Ye, Hang Li, Yuhan Liu, Chunchun Meng, Jie Zhu, Guangqing Liu & Chuanfeng Li

  2. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China

    Yumeng Ye & Yong Wang

  3. College of Veterinary Medicine, Xinjiang Agricultural University, Wulumuqi, 830052, China

    Yuhan Liu

Authors
  1. Yumeng Ye
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  2. Yong Wang
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  3. Hang Li
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  4. Yuhan Liu
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  6. Jie Zhu
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  7. Guangqing Liu
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  8. Chuanfeng Li
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Contributions

CF Li and GQ Liu conceived and designed the details of the experiments. YM Ye and Y Wang performed the data collection and experiment. H Li and YH Liu analyzed the data. CF Li and YM Ye wrote the manuscript. CC Meng and J Zhu assisted in the experimental operations and revision of the manuscript. All authors read and approved the manuscript.

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Correspondence to Guangqing Liu or Chuanfeng Li.

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The authors declare no competing interests.

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Yumeng Ye and Yong Wang contributed equally to this work.

Responsible Editor: Fernando R. Spilki

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Ye, Y., Wang, Y., Li, H. et al. Genetic characterization of duck hepatitis B viruses from Anhui Province, China. Braz J Microbiol 54, 3299–3305 (2023). https://doi.org/10.1007/s42770-023-01120-0

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  • DOI: https://doi.org/10.1007/s42770-023-01120-0

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