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Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil

  • Veterinary Microbiology - Research Paper
  • Published:
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Abstract

This study is aimed at detecting Feline paramyxovirus (FPaV) and Feline morbillivirus (FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil.

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Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, PO Box 10011, Londrina, Paraná, 86057-970, Brazil

    Luciana C. Balbo, Juliana T. T. Fritzen, Elis Lorenzetti, Amauri A. Alfieri & Alice F. Alfieri

  2. Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Elis Lorenzetti, Amauri A. Alfieri & Alice F. Alfieri

  3. Post Graduate Program in Animal Health and Production, Universidade Pitágoras Unopar, Arapongas, Paraná, Brazil

    Elis Lorenzetti & Andressa M. Jardim

  4. Clinovet Dumont, Londrina, Paraná, Brazil

    Thais N. S. Medeiros

Authors
  1. Luciana C. Balbo
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  2. Juliana T. T. Fritzen
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  3. Elis Lorenzetti
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  4. Thais N. S. Medeiros
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  5. Andressa M. Jardim
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  6. Amauri A. Alfieri
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  7. Alice F. Alfieri
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Correspondence to Alice F. Alfieri.

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Responsible Editor: Fernando R. Spilki

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Balbo, L.C., Fritzen, J.T.T., Lorenzetti, E. et al. Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil. Braz J Microbiol 52, 961–965 (2021). https://doi.org/10.1007/s42770-021-00434-1

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  • DOI: https://doi.org/10.1007/s42770-021-00434-1

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