Natural Plant By-Products and Mulching Materials to Suppress Weeds and Improve Sugar Beet (Beta vulgaris L.) Yield and Quality

To reduce the use of herbicides in agriculture, there is an urgent need to look for cost-effective and environmentally friendly non-chemical methods to control weeds in field crops. Plant by-products could be exploited directly as plant residues or indirectly as plant extracts for weed control. Thus, the objective of this study was to evaluate the effectiveness of several plant wastes and extracts in controlling weeds of sugar beet (Beta vulgaris subsp. vulgaris) with enhancing yield and quality. Three weed control groups of twelve practices were examined. The first weed control group included three aqueous extract concentrations (15, 20, and 25%) of Plectranthus amboinicus L. The second weed control group involved six soil mulching plant materials [rice (Oryza sativa L.) straw, wheat (Triticum aestivum L.) hay, peanut (Arachis hypogaea L.) straw, mango (Mangifera indica L.) leaves, flax (Linum usitatissimum L.) meal, and soybean (Glycine max (L.) Merr.) meal], while the third group comprised of desmedipham/ethofumesate/lenacil/phenmedipham (DELP) herbicide 1.5 L ha−1 [desmedipham 70.5 g active ingredient (ai) ha−1 + ethomesufate 112.5 g ai ha−1 + lenacil 40.5 g ai ha−1 + phenmedipham 90 g ai ha−1], hoeing, and unweeded check. During a 2-year field trial (2019/20 and 2020/21), the 12 weed control treatments arranged in a randomized complete block design (RCBD) and replicated three times. Compared to the recommended applications (hoeing and herbicide), reduction averages of both seasons for total weeds biomass were 46.3–54.2%, 37.3–46.4%, and 23.0–34.4% due to rice straw, wheat hay, and mango leaves, respectively. Root and sugar yields of sugar beet divulged the highest values with hoeing in both seasons statistically equaling (P ≥ 0.05) rice straw and wheat hay for root yield and wheat hay for sugar yield in the first season. Except flax meal, all weeded treatments in the first season as well as mango leaves, wheat hay, peanut straw, and hoeing in the second one caused significant (P ≤ 0.05) reductions in sodium content of beet juice. Wheat hay, mango leaves, and hoeing in both seasons, in addition to rice straw, peanut straw, and herbicide in the first season, were the most efficient practices for improving sucrose % and extractable sugar %. Compared to hoeing, reductions in net return were –286.7 and –320.0 ($ ha−1) by percentage of 6.5 and 7.2, due to mulching soil by rice straw and wheat hay, respectively. Recycling the plant by-products such as wheat hay, rice straw, peanut straw, or mango leaves in the form of soil mulch could serve as safe and eco-friendly tools in weed control programs of sugar beet. The beneficial effect of mulching extended to enhance root and sugar yields with low impurities. Since the plant wastes are available in the farm, better revenues will be gained for sugar beet growers. Further investigations related to the use of plant extracts as natural herbicide should be performed to reach acceptable levels for weed control.


Introduction
Sugar beet (Beta vulgaris L.) ranks as the second most important sugar crop worldwide after sugar cane. (Saccharum officinarum L.) (Brar et al. 2015). Sugar beet is a temperate crop and its root contains a high amount of sucrose (Paul et al. 2019). It is still one of the main sources for sucrose extraction where approximately 30% for human consumption sugar of the world is contributed by the sugar beet crop (Bairagi et al. 2013). The total global cultivated acreage of about 4.4 Mt produces approximately 253 Mt of sugar beet roots that provides ~ 30% of the gross world's requirements of white sugar (FAO 2022). However, sugar beet production worldwide is frequently faced ecological challenges (Abd El-Mageed et al. 2022;Makhlouf et al. 2022) and biotic stresses such as weeds. Since sugar beet is highly sensitive to weed competition during the initial growth stages, effective weed control should be implemented (Jalali and Salehi 2013;Marwitz et al. 2014). Sugar beet plants must be free of weeds until 46 to 54 days after emergence to prevent yield loss over 5%, while the presence of weeds caused decrease in root yield by 60-99% (Dogan and Adem 2018). Accordingly, weed management should be paid attention with application of new methods are required in order to identify the best weed control timing and to minimize costs. Herbicides application can result in low crop interference with weeds (Saudy 2014;Saudy and Mubarak 2015;El-Metwally and Saudy 2021b;Saudy et al. 2021b). The spectrum of herbicides used in sugar beet is rather narrow and further restrictions are expected in the near future (Jursík and Holec 2019). Also, no single chemical herbicide can control all weeds in sugar beet fields. Moreover, herbicides led to rapid selection of many resistant weed populations (Heap 2018). Thus, continuous dependence on herbicides application should be reduced and alternative tools of weed control should be adopted (Saudy and El-Metwally 2009;Saudy and El-Bagoury 2014;Saudy 2015;Papapanagiotou et al. 2019).
Therefore, alternative non-chemical weed control options should be evaluated.to choose the best one. As a conventional tactic, hoeing is a clean mechanical method for eliminating weeds in in row crops which achieve high weed control efficiency (Abd El Lateef et al. 2021;. However, hoeing is still hard and costly due to it requires high effort and labor expense. In farms that are organically managed, exploiting the agricultural wastes is regarded as a useful act for nutrients recycling with clean environment and production of healthy plants Elgala et al. 2022). Plant wastes represent a serious source of carbon dioxide emissions. Recycling the agricultural wastes as soil mulching or natural plant extract could be exploited in weed management programs.
Several benefits could be gained by application of soil mulching. Mulching has been increasingly practiced improving crop yield by enhancing precipitation use efficiency (Awodoyin and Ogunyemi 2005;, conserving soil moisture (Zhao et al. 2009;Salem et al. 2021) and regulating soil temperature (Kader et al. 2019). Moreover, Wang and Xing (2016) reported that the soil water content, yield and fertilizer use efficiency in the mulching treatment were significantly higher than that of non-mulching one. Increment in crop productivity with applying straw mulch is largely attributed to maintain soil moisture (Gan et al. 2013), decreasing soil bulk density (Hassan et al. 2007) and activating plant root system (Huang et al. 2012). Concerning weed problems, straw mulch could be used as an efficient tactic of reducing weed emergence and growth . Decreases in weed population and dry biomass with improving weed control efficiency were observed owing to organic materials application (Yadav et al. 2015;Saudy et al. 2021c). Thus, various crop yields were increased by about 80-135% as a result of mulch application (El-Metwally and El-Wakeel 2019). Mulching the soil by rice straw caused 48.9% increase in potato tuber yield (Bhullar et al. 2015). Since soil mulches, i.e., peanut straw and herbicide are similar for inhibiting weed growth and increasing the marketable yield, water use efficiency, and economic returns, the farmers are advised to use soil mulching instead of herbicides as a clean and eco-friendly method for weed control .
The use of eco-friendly natural plant compounds is a promising approach to replace, partially at least, synthetic herbicides in weed management programs. For shrinking the dependence on the synthetic pesticides and maintaining the agroecosystems, allelopathy phenomenon as an ecologically safe method could be exploited in weed control with improving crop yields (Hegab et al. 2008. Allelopathic effect could be regarded as a sustainable pattern for biologically weed management (Arora et al. 2015) by liberating chemical compounds from different plant organs influencing adversely the growth of other plants (Delcour et al. 2015). Owing to the integration with aqueous plant water extracts, herbicide addition rate could be lowered with achieving distinctive efficacious weed control (Khan et al. 2012).
Since weeds are great consumer for soil water and nutrients, numerous attempts have been adopted to combat weeds in the economic field crops. Moreover, using mulches of allelopathic crops is an important tool to suppress weed flora and improve crop yield. However, the role of different mulches in suppressing weed flora and improving the root yield and quality of sugar beet has infrequently been investigated. The current research hypothesized that using various plant wastes in the form of mulching or aqueous extracts will have better efficiencies for controlling weeds than conventional methods, hence improving sugar beet yield and quality. Therefore, this study aimed to assess the effect of diversified mulch types and aqueous extract concentrations of Plectranthus amboinicus L. compared to the common practices (herbicide or hoeing) on weed growth, sugar beet yield and quality keeping in mind the economic feasibility.

Site Description
During the winter seasons of 2019/20 and 2020/21, two field experiments were conducted at the Experimental Farm, National Research Centre El-Behaira Governorate, Egypt (30.30° N,30.18° E and 21 m above sea level) (see Fig. 1). The experimental soil was sandy and the mechanical and chemical analysis of the soil are presented in Table 1. Based on the soil taxonomy (IUSS Working Group WRB 2015), the soil is order Entisols and suborder Psamments. The site is classified as an arid zone with cool winters and non-significant precipitation. As an average of the two seasons, the mean values of daily air temperature, wind speed, relative humidity, precipitation, and solar

Crop Establishment and Management
Soil was prepared at a tillage depth of 30 cm with incorporating the ordinary calcium superphosphate (15.5% P 2 O 5 , 61.2 g P kg −1 ) at a rate of 74.0 kg P ha −1 . Nitrogen fertilizer was applied at a rate of 240.0 kg N ha −1 as ammonium nitrate (33.5% N) in 5 equal portions, 30, 45, 60, 75, and 90 days after sowing (DAS). Potassium fertilizer was added at a rate of 60.0 kg K ha −1 as potassium sulfate (48% K 2 O, 398.4 g kg −1 ) in 3 equal portions, 60, 75, and 90 DAS. Trickle irrigation system was set up consisting of control head (media and screen filters, pressure gauges, and control valves), main and sub-main lines (main line is PVC of 75.0-mm diameter up to 6.0 bar pressure, 41.0-m length, and sub-main line is PVC pipe of 50.0-mm diameter up to 6.0 bar pressure) as well as lateral lines are polyethylene tubes of 16-mm diameter (with built in emitters), 30.0-cm emitter spacing, and manufacturing emitter discharge 4.0 L h −1 , at operating pressure of 1.0 bar. Sugar beet cultivar Baraka (multi germ) was sown manually in hills 25 cm apart at a rate of 4.8 kg ha −1 in rows on the 21st and 29th of October in 2019/20 and 2020/21 seasons, respectively. Seeds were drilled on one side of the ridge in hills 25 cm apart. For obtaining one plant per hill, plants were thinned twice at 30 and 50 DAS.

Plant Materials and Experimental Design
The experiment included 12 treatments represented in three concentrations of aqueous extract of Plectranthus amboinicus L. six soil mulching plant materials, in addition to three check treatments (herbicide and hoeing as positive checks and unweeded as negative check). The experimental design was randomized complete block design (RCBD) in four replicates. The experimental unit included 5 ridges, 70 cm apart and 3.0 m length, occupying an area of 10.5 m 2 . In the second season (2020/21), new plots were established in an adjacent but different area of the same site to avoid any residual effects of the fertilizer on the studied parameters and to actually repeat the experiment in time. Details of the treatments could be briefly described as follow: Aqueous Extract P. amboinicus L. leaves were collected from Egyptian gardens, washed with tap water, then with distilled water to eliminate dust. After air drying in the shade, the dried leaves were finely powdered by an electric mill. The dry powdered leaves (1500 g) were transferred to labeled beakers to which 6 L of distilled water were added and allowed to soak for 48 h. Then the produced extract was collected and filtered through a very fine mesh and pushed through the mesh carefully for complete extraction. The produced extract was at a 25% concentration. Part of the extract remained as it was (2 L of 25%) and the remaining extract was diluted with distilled water, to concentrations of 15 and 20% for each extract. The process of extraction was repeated according to need to ensure that the extracts were fresh. The analysis described by Srisawat et al. (2010) proved that P. amboinicus L. extract contained total phenols (29.5 mg g −1 ) and total flavonoids (11.2 mg g −1 ) on dry weight base. The prepared aqueous extract solution with different concentrations was sprayed at a rate of 480 L ha -1 . Extract spraying was applied two times at 15 and 30 DAS.

Hoeing
Hand hoeing thrice at 20, 40 and 65 DAS was performed.
Unweeded In plots of unweeded treatment the weeds left to grow freely until the harvest.
Both of aqueous extract and herbicide were separately sprayed using Cooper Pegler CP3 Classic 20lt Professional Knapsack Sprayer fitted with a flat-fan nozzle and calibrated to deliver 480 L water ha -1 .

Weed Biomass
Four 0.25 m 2 (0.5 m × 0.5 m) wooden quadrats were placed in each plot in area with uniform weed flora and away from the margins. Using the quadrats with a size of 1 m 2 , weeds were hand pulled in each plot at 90 DAS and separating them by species and placing them in numbered paper bags. Thereafter, weed samples were air-dried (for 12 days) and oven-dried (at 70° C until constant weight). Moreover, the weed samples were then weighed to determine the dry weed biomass per unit area.

Agronomic Traits
At 90 DAS, leaf greenness expressed in SPAD value of the uppermost fully expanded leaf was determined by chlorophyll meter (SPAD-502Plus) according to Süß et al. (2015). Moreover, a sample of ten plant was taken from each experimental unit to estimate leaves fresh weight, root fresh weight, root length and root diameter. At harvest (on May 15 and May 20 in 2019/20 and 2020/21 seasons, respectively) whole plants of each experimental unit were uprooted to estimate root yield ha −1 .

Sugar Yield and Quality Traits
Twenty roots from each plot were randomly taken to determine root quality and technological characteristics at Quality Control Laboratory, El-Nubaria Sugar Factory, El-Behera, Egypt. Sucrose % was determined using Saccharometer according to the method described in AOAC (2012). According to Cooke and Scott (1993) impurities (potassium (K), sodium (Na), and alpha amino nitrogen (α-amino N) were estimated. Sugar lost to molasses % (Eq. 1) and alkalinity coefficient (Eq. 2) were calculated as described by Deviller (1988). Extractable sugar % was computed by Eq. 3 (Dexter et al. 1967). Moreover, juice purity using Eq. 4 was estimated (Cooke and Scott 1993). After that, sugar yield ha −1 was calculated using Eq. 5 as reported by Deviller (1988).

Economic Profitability
According to Cimmyt (1988), the economic evaluation was estimated by calculating the cost of cultivation for different agro-inputs, i.e., labors, fertilizers, irrigation, mulching, insect control, harvesting, and other necessary materials.
Returns of each treatment were calculated ($ ha −1 ) on the basis of local market prices using Eqs. 6 and 7 as follows: The average prices were taken from the local market where the price of one ton of sugar beet root was 66.67 ($); and the fixed costs of cultivation were 1066.67 ($ ha −1 ).

Statistical Analysis
The obtained data of the two seasons were subjected to the analysis of variance (ANOVA) according to Casella (2008), using Costat software program, Version 6.303 (2004), CoHort Software, Monterey. Years and weed control treatments were considered as fixed effects while replications (blocks) were considered as random effects. Means separation was performed only when the F-test indicated significant (P ≤ 0.05) differences among the treatments, based on Duncan's multiple range test.
Sugar beet root yield data (t ha -1 ) were correlated with total weed dry weight data (g m -2 ). According to the following linear model, correlation was performed at a significance level of a = 0.05 using STAT GRA PHICS Centurion XVI: where y is sugar beet root yield data, x is total weed dry weight data, a is the intercept, and b is the slope of the regression line.

(7)
Net return = Gross returns − fixed and variable cost of crop production recorded with rice straw, wheat hay and mango leaves. Compared to the recommended applications (hoeing and DELP, reduction averages of both seasons for total weeds biomass were 46.3-54.2%, 37.3-46.4%, and 23.0-34.4% due to rice straw, wheat hay, and mango leaves, respectively.
Despite aqueous extract of P. amboinicus L. gave less reduction in weed biomass than mulching, all extraction concentrations surpassed the weedy check and reduced total weed biomass by 57.4, 61.2 and 65.2% with the concentrations of 15, 20, and 25%, respectively.

Sugar Beet Growth and Yields
Sugar beet growth parameters measured at 90 DAS (Table 3) and yields (Table 4) significantly affected by weed control treatments in 2019/20 and 2020/21 seasons. Wheat hay showed the maximum leaves fresh weigh plant −1 in both seasons. Also, wheat hay along rice straw, peanut straw, and hoeing in both seasons, in addition to mango leaves in the first season exhibited the maximum increases in SPAD value. Except the extract concentration treatments in both seasons and soybean meal or flax meal in the second season, the other weeded treatments were similar for enhancing root fresh weight surpassing the unweeded. Hoeing in both seasons as well as rice straw, wheat hay, and DELP herbicide in the second season showed the longest roots of sugar beet. The widest roots expressed in root diameter were observed with DELP herbicide and wheat hay in both seasons, in addition to peanut straw in the second season.
All weeded practices surpassed the unweeded for improving root and sugar yields in both seasons. Root Table 2 Biomass of broad-leaved and grass weeds (g m −2 ) infested sugar beet as affected by weed control treatments in 2019/20 and 2020/21 seasons P. amb. 15, P. amb.20, and P. amb.25: Plectranthus amboinicus L. leaves extract at concentrations of 15, 20 and 25%, respectively. DELP herbicide: desmedipham 70.5 g ai ha −1 + ethomesufate 112.5 g ai ha −1 + lenacil 40.5 g ai ha −1 + phenmedipham 90 g ai ha −1 . Values are the mean of 3 replicates ± standard errors. Different letters within columns refers that there are significant variations at 0.05 level of probability. Means were separated based on Duncan's multiple range test (P ≤ 0.05)

Treatment
Broad-leaved Grass and sugar yields of sugar beet divulged the highest values with hoeing in both seasons statistically equaling rice straw and wheat hay for root yield and wheat hay for sugar yield in the first season. Furthermore, the other mulching materials, especially peanut straw and mango leaves, came in the second order for achieving distinctive enhancements in root and sugar yield. According to the results of the linear regression analysis performed, weed competition affected sugar beet root yield. Negative and strong correlation was observed between root yield data and total weed dry weight ( Fig. 3; n = 36; y = 83.763-0.0553 × x; p ≤ 0.001; R = − 0.947; R 2 = 0.8977).

Sugar Impurities
Except α-amino N in the second season, all other impurities significantly affected by weed control treatments in both seasons (Table 5). Potassium showed the maximum concentration with flax meal, soybean meal, and wheat hay in the first season and with wheat hay, P. amb.25, P. amb.20, and hoeing in the second one. Except flax meal, all weed control treatments in the first season as well as mango leaves, wheat hay, peanut straw and hoeing in the second season caused significant reductions in sodium content. The highest α-amino N content was obtained with flax meal in the first season. Hoeing and P. amb.20 recorded the maximum alkalinity coefficient in the first and second seasons, respectively.

Juice Quality
Wheat hay, mango leaves, and hoeing in both seasons, in addition to rice straw, peanut straw, and DELP herbicide in the first season were the most efficient practices for improving sucrose % and extractable sugar % (Table 6). Hoeing, DELP herbicide, and mango leaves (in both seasons); and wheat hay, rice straw, peanut straw P. amb.25, and P. amb.20 (in 2019/20 season) as well as flax meal (in 2020/21 season) gave the best juice purity %. The highest values of sugar lost to molasses % were obtained with flax meal and soybean meal in the first season as well as unweeded, hoeing, wheat hay, P. amb.25, and P. amb.15 in the second season.

Economic Profitability
The economic analysis divulged that there were higher increases in gross return, net return and total cost owing to the weeded practices than the unweeded (Table 7). Hoeing exhibited the maximum gross and net returns, while soybean meal or flax meal manifested the maximum costs. Rice straw followed by wheat hay were the closest to the hoeing in achieving the highest revenues. Compared to hoeing, reductions in net return amounted to -286.7 and -320.0 ($ ha −1 ) by percentage of 6.5 and 7.2, due to mulching soil by rice straw and wheat hay, respectively.

Discussion
In order to maintain adequate growth factor, i.e. water, and nutrients for crop growth, reasons of loss of growth requirements should be precluded. Since weeds are considered one of the major competitors for environmental factors, they should be combated (El-Metwally and Saudy 2021a). There is no doubt that weeds exemplify emphatic biotic stress toward crop plants, particularly at early growth stages. Accordingly, application of practices which can suppress weed growth early is a crucial act in this situation. The current investigation suggests sundry safe weed control patterns which may have different mechanisms for quenching weeds in sugar beet. Controlling weeds in sugar beet fields depends mainly on the use of mechanical and chemical methods. In this respect, classical weed control methods i.e. hoeing (as Table 3 Sugar beet growth parameters as affected by weed control treatments after 90 days from sowing in 2019/20 and 2020/21 seasons P. amb.15, P. amb.20, and P. amb.25: Plectranthus amboinicus L. leaves extract at concentrations of 15, 20 and 25%, respectively. DELP herbicide: desmedipham 70.5 g ai ha −1 + ethomesufate 112.5 g ai ha −1 + lenacil 40.5 g ai ha −1 + phenmedipham 90 g ai ha −1 . Values are the mean of 3 replicates ± standard errors. Different letters within columns refers that there are significant variations at 0.05 level of probability. Means were separated based on Duncan's multiple range test (P ≤ 0.05).   (Saudy et al. 2020). However, despite hoeing still being a conventional weed control in row crops, manual labor has become scarce and expensive . DELP herbicide caused reductions of 90.2, 88.9, and 89.7% (averages of the two seasons) in dry weight of broad leaf, grass, and total weeds, respectively. In this regard, application of acetolactate synthase (ALS) inhibiting herbicides achieved excellent efficacy on common sugar beet weeds (Gotze et al 2018). Furthermore, Jursík et al. (2020) recorded that DELP herbicide completely controlled Amaranthus retroflexus L., Echinochloa crus-galli L., and Chenopodium album L. in sugar beet.
Findings of the current work clarified that using different plant wastes as soil covering achieved significantly reduction in weed biomass. It should be mentioned that in widely spaced row crops such as sugar beet, severe weed competition occurs. In this situation, the allowable penetrated sunlight to the soil surface increases, along with increased crop-weed competition for soil minerals, soil moisture, and CO 2 , and it becomes more available to weeds. This promotes the weeds to grow better and dramatically spread. Conversely, blocking light to reach the soil surface by mulching directly reduced subsequent germination and suppressed weed growth (Monaco et al. 2002). Due to blocking light penetration, straw mulch prevented the seed germination and inhibited the emerged weed seedlings growth (Chang et al. 2016). Weed density in straw-mulched field was less than that of non-mulched one (Sinkevičienė et al. 2009). Furthermore, several plant residues decreased weed competition because of their allelopathic potentiality (Anzalone et al. 2010). The negative impacts of weeds could be dwindled by employing the allelopathic potential of crops using mulches (Riaz Marral et al. 2020). Significant increase in relative water content and photosynthesis efficiency and decrease in weed competition were obtained with using of soil mulching (Abd El-Mageed et al. 2016;El-Metwally et al. 2022).  Therefore, sugar beet growth and yield and sugar quality were improved owing to mulching application.
As an attempt to exploit the allelopathy phenomenon, the current research proved that the aqueous extract of P. amboinicus L. particularly at a high rate (25%), involved significant amount of phenolics, thus it displayed reasonable prorated repressive impact on weeds associated sugar beet plants. Various types of phenolic compounds such as syringic acid, chlorogenic acid, ferulic acid, vanillic acid, p-coumaric acid, protocatechuic acid, caffeic acid, benzoic acid, and gallic acid are typified by allelopathic properties (Hassan et al. 2012). Owing to existence of phenolics, aqueous water extracts and plant residues mulch may contribute in decreasing weed infestation (Farooq et al. 2017;Naeem et al. 2022). Allelochemicals can disrupt the metabolism processes of recipient plants such as photosynthesis, cell division, respiration and protein synthesis (Duke and Dayan 2006). Allelopathic chemicals cause physiological injuries due to unrestrained production and the accumulation of reactive oxygen species, ROS (Bogatek and Gniazdowska 2007). ROS causes cell structure disintegration by lipids peroxidation, DNA and proteins impairment, and enzyme inertia (Wang et al. 2017). Accordingly, allelochemicals can demoralize the growth of weeds through photosynthesis inhibition, free radical production, chlorophyll deterioration, deactivation of enzymatic systems, and cell membrane turmoil of the target plants (Ghanizadeh et al. 2014). However, still, the efficacy of natural plant extracts for weed control requires more improvements.
Despite hoeing was the superior practice for obtaining the maximum benefits of sugar beet cultivation, soil mulching materials especially rice straw and wheat hay, should not be neglected. In this respect, besides mulching as a clean method, it achieved benefits higher than herbicide application. Also, in addition to the financial interests returning the agricultural wastes in the form of mulching achieves another advantage related to lowering greenhouse emissions. Economically and environmentally sustainable weed control alternatives, such as non-synthetic or natural mulch, can provide many benefits, including weed suppression and delayed weed seedling emergence (Abouziena et al. 2015;El-Metwally and El-Wakeel 2019). Distinctive improvement in yield and quality was achieved using soil mulches particularly peanut mulch. .

Conclusions
The current study revealed that different soil mulching materials significantly differed in their potentiality to suppress weed growth and enhance yield and quality of sugar beet. The results proved that exploiting the plant by-products as mulching showed several advantages via suppressing the growth of various weed types, increasing sugar beet yield potential and quality with high revenues. Accordingly, using wheat hay, rice straw, peanut straw, or mango leaves as safe and eco-friendly methods to control weeds is promising in sugar beet cultivation. Instead of herbicides, the allelopathic effect of Plectranthus amboinicus L. aqueous extract could be incorporated into weed management strategies as an additional, more cost-effective and eco-friendly method that will help to save the environment. However, the use of natural plant extracts, such as Plectranthus amboinicus L. in weed control still requires further investigations with the aim of increasing their efficiency to persuade the farmers to use them as a tool for weed control.

Conflict of Interest
The authors declare no competing interests.
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