Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

Trans-anethole possesses several biological and pharmacological effects. It is also used as masking agent in household products and as a flavoring agent in food. Clausena anisata (Willd.) Hook. f. ex Benth. leaves are reported as a source of trans-anethole that makes their anise-like odor. This work sought to optimize the dynamic maceration of C. anisata leaves to maximize the trans-anethole content. The circumscribed central composite experimental design was applied to investigate the effects of extraction temperature and time on extraction yield, trans-anethole content in the extract, and trans-anethole content in the plant raw material. The results showed that the extraction yield was high when C. anisata leaves were extracted over a long extraction time, while the extraction temperature had a lesser effect on the extraction yield. Trans-anethole content in the extract and plant raw material determined using a linear, specific, precise, and accurate HPLC method was high when a medium extraction temperature with a short extraction time was used. The optimal condition that maximized trans-anethole content involved an extraction temperature of 61.8 °C and an extraction time of 12.9 min, respectively. The percentage error of the prediction conducted by computer software was low, suggesting that the prediction was highly accurate. In conclusion, the optimal condition of dynamic maceration obtained from this work could be used as a guide for maximizing trans-anethole content from C. anisata leaves.

Clausena anisata leaves generate an anise-like odor, which is the smell of a major essential oil: trans-anethole. Trans-anethole is a clear, colorless to amber-colored liquid with an anise-like flavor. It is slightly soluble in water but highly soluble in ethanol. It is 13 times sweeter than sugar and used in many alcoholic drinks. Further, it is also used as a masking agent in household products and as a flavoring agent in food. According to its pharmacological effects, it possesses several properties such as antioxidant, antimicrobial, anthelmintic, insecticidal, expectorant, spasmolytic, antinociceptive, anti-inflammatory, gastroprotective, and sedative activities [21].
The extraction procedure is an essential step of plant composition analysis and the study of the activity of the essential oils. A suitable extraction condition provides the desired plant bioactive constituents with a small degree of degradation [22]. As such, the selection of an extraction condition is an important parameter. Design of Experiment (DOE) is a tool that is used to systemically examine different types of problems occurring within research, development, or production step [23]. DOE is a multipurpose tool that can be used in many circumstances, i.e., design for comparisons, screening of variables, transfer function identification, optimization, and robust design [24]. It is the most popular tool in various scientific areas, such as medicine, engineering, biochemistry, genetics, physics, astronomy, and computer sciences. [24]. The modern experimental design exhibited superior advantages compared with the traditional One Factor at a Time (OFAT) trialing such as less time and financial consuming, less pharmaceutical substance use, interaction effect can be identified, and surface response can be characterized [25]. Application of DOE in the extraction process of herbal plant is an interesting methodology. To the best of our knowledge, there is no work reported about the optimization of extraction of trans-anethole from C. anisata leaves. The objective of this work was to optimize the dynamic maceration of C. anisata leaves to maximize the trans-anethole extracted. The circumscribed central composite experimental design was applied to investigate the effects of two factors-extraction temperature and extraction time-on the three responses of extraction yield, trans-anethole content in the extract, and trans-anethole content in the plant raw material. The authors expect that the optimal condition obtained from this work could be used as a guide for maximizing trans-anethole from C. anisata leaves to use as ingredient of food, beverage, or herbal products.

Materials
Trans-anethole (purity 99.6%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Absolute ethanol (AR grade) and methanol (HPLC grade) were purchased from Duksan Pure Chemicals (Kyunggi, Korea). C. anisata leaves were collected in November 2018 from Sing Buri Province, Thailand. The plant sample was identified by Chair Prof. Dr. Nijsiri Ruangrungsi to ensure the right plant species. The voucher specimen was coded as CM-CA003-1-11-2018 was then deposited at the Drug and Herbal Product Research and Development Center, College of Pharmacy, Rangsit University.

Optimization of dynamic maceration
A circumscribed central composite experimental design was applied in this work. The effects of the two factors [i.e., extraction temperature (X 1 ) and extraction time (X 2 )] on the three responses [i.e., extraction yield (Y 1 ), transanethole content in extract (Y 2 ), and trans-anethole content in the plant raw material (Y 3 )] were investigated. Eight non-center points (Conditions 1-8) with four center points (Conditions 9-12) were selected. Extraction temperature and extraction time were varied for five levels from 40 to 80 °C and 10 to 30 min, respectively. The coded and actual values of the 12 model conditions obtained from the experimental design are shown in Table 1.
Collected dried leaves of C. anisata were pulverized and passed through 20-mesh sieve. Plant powders with particle size of approximately 850 μm were obtained. Then, 10 g of C. anisata leaf powder was mixed with 100 mL of absolute ethanol in a 250-mL Erlenmeyer flask. The mixture was dynamically macerated in a water bath (WNB 14; Memmert GmbH + Co. KG, Büchenbach, Germany) equipped with a shaking device (SV 1422; Memmert GmbH + Co. KG, Büchenbach, Germany) according to the condition shown in Table 1. The shaking device was programmed with an agitation rate of 160 strokes/min. Then, the slurry was filtered to collect the filtrate. The macerated leaf mixture was extracted again with the same procedure. The extraction was performed three times, the three filtrates were pooled, and the solvent was removed using a rotary evaporator (Buchi Labortechnik AG, Flawil, Switzerland). The extraction yield was calculated.
The trans-anethole content was analyzed using HPLC. The extract was diluted in methanol into a concentration of 0.5 mg/mL then filtered and injected into the HPLC instrument. Results of the trans-anethole content in the extract and plant raw material were reported. The three responses were optimized using the Design-Expert ® version 11.0 software program (Stat-Ease, Inc., Minneapolis, MN, USA). Contour plots and response surfaces of the model conditions of the above three responses were constructed, and the prediction equations were reported. The criterion for optimization was to "maximize" the transanethole content in the extract and plant raw material. The condition with the highest desirability value was selected as the optimal condition. The optimal condition provided by the Design-Expert ® software was used to reextract the C. anisata leaves to confirm the accuracy of the prediction.

HPLC analysis of trans-anethole
The analysis of trans-anethole content was done using the Agilent 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA). Isocratic elution was performed using ACE ® Generix™ (150 × 4.6 mm, i.d., 5 μm) (Advanced Chromatography Technologies Ltd., Aberdeen, Scotland). The column temperature was controlled at 25 °C. Water and methanol in a ratio of 25:75 v/v were used as a mobile phase. The injection volume was 10 μL, while the detection wavelength was 260 nm.

Linearity and range
A stock solution of standard trans-anethole in the concentration of 1 mg/mL was prepared using methanol as a solvent. Then, it was diluted with the same solvent to the following seven concentration levels: 1, 5, 10, 25, 50, 75, and 100 μg/mL. Each solution was filtered and then analyzed by HPLC, and each underwent measurements performed in triplicate. The calibration curve of standard trans-anethole was produced, and a coefficient of determination (R 2 ) was reported.

Limit of detection (LOD) and limit of quantitation (LOQ)
The 10 injections of a blank solution of methanol were analyzed by HPLC. The standard deviation (SD) was calculated. LOD and LOQ are calculated as per the equations shown in Eqs. 1 and 2: where σ is the SD of the response of the blank solution and S is the slope of the calibration curve.

Specificity
The specificity of the analysis was evaluated based on the ultraviolet (UV) spectrum of the peak of the compound as compared with the UV spectrum of the peak of the reference standard. UV spectrums of the three peak regions (top, upslope, and downslope) of the peak of trans-anethole in the C. anisata leaf extract were compared with the UV spectrum of the peak of standard trans-anethole. The method was specific when UV spectrums of the peak of trans-anethole in C. anisata leaf extract were similar to the UV spectrum of the peak of standard trans-anethole.

Precision
The precision analysis was performed for intraday precision and interday precision. Three concentration levels of standard trans-anethole (25,50, and 75 μg/mL) were analyzed. The three injection datasets of each concentration level (intraday) and the nine injection datasets (interday, three each day for 3 days) of each concentration level were used to calculate the percent relative SD (%RSD). The data obtained from the analysis on the same day and among the three different days indicated the intraday precision and interday precision, respectively.

Accuracy
The individual three concentration levels of standard trans-anethole (25,50, and 75 μg/mL) were added to the C. anisata extract solution with a known amount of transanethole. Each concentration level was analyzed in triplicate. The percent recovery of each concentration level was reported.

Results
The reliability of chemical analysis results was assured by reliable HPLC method. So, the HPLC method validation was performed in this work. A calibration curve of standard trans-anethole is shown in Fig. 1. The linear equation was y = 11,335,815x -11,187,004 (R 2 = 0.9994) in the concentration range of 1-100 μg/mL. LOD and LOQ were 8.4 ng/ mL and 25.4 ng/mL, respectively. Figure 2 presents the HPLC chromatograms of standard trans-anethole and C. anisata extract. The simple isocratic system could analyze the trans-anethole content, which was eluted at the retention time of 6.8 min. According to the specificity, this method was specific due to the similarity between the UV spectrums of the three peak regions (i.e., top, upslope, and downslope) of the peak of trans-anethole in C. anisata leaf extract and the UV spectrum of the peak of standard transanethole (Fig. 3). The two maximum absorbance values of trans-anethole were observed at 204 nm and 258 nm. The precision and accuracy results are shown in Table 2. The intraday precision was lower than 0.1%, while the interday precision was lower than 1%, and percent recovery was in the range of 96.94-101.25%. As such, these results  (Table 1). Figure 4 displays the contour plots and response surfaces of the model conditions of extraction yield, transanethole content in the extract, and trans-anethole content in the plant raw material. Furthermore, coded and actual equations of each response are shown as follows. The model conditions of extraction yield, trans-anethole content in the extract, and trans-anethole content in the plant raw material were fitted to the linear, quadratic, and quadratic mathematics models, respectively. The coded and actual equations are shown in Eqs. 3-8. Coded equations:   According to the contour plot, the response surface (Fig. 4a), and the above equations, extraction time has a positive effect on the extraction yield, while the extraction temperature had a small impact. Figure 4b and c reveals that a high trans-anethole content in the extract and in plant raw material was achieved when a medium extraction temperature with a short extraction time was used. Figure 5 shows the bar graph of desirability value. The optimal condition that ensured the simultaneous high of trans-anethole content in extract and plant raw material was an extraction temperature of 61.8 °C and an extraction time of 12.9 min, with a desirability value of 0.794. The accuracy evaluation by comparison between the predicted value and experimental value found that the prediction by Design-Expert ® provided a low percent error. Overall, the percent errors of all responses were lower than 20% (Table 3).  5 Desirability chart, when the temperature and time were set as "in range, " extraction yield was set as "none, " and trans-anethole content in extract and plant raw material were set as "maximize"

Discussion
To the best of our knowledge, this is the first work that optimized the extraction parameter of dynamic maceration of trans-anethole from C. anisata leaves. Furthermore, application of modern experimental design provided less time and financial consuming, less chemical use, interaction effect can be identified, and surface response can be characterized [25]. Trans-anethole is volatile by nature and could be degraded by a high extraction temperature with a long extraction time. Extraction temperature and extraction time commonly have a positive impact on the extraction efficiency of thermostable plant compounds. For example, increasing the extraction temperature from 40 to 90 °C and the extraction time from 1 to 4 h increased the extraction yield of polysaccharides of Gynura bicolor leaves [26], while increasing the extraction temperature from 30 to 80 °C and the extraction time from 20 to 180 min increased the tea protein extraction rate of tea residues [27]. Moreover, Yuan et al. [28] revealed a similar result for the optimization of polysaccharides from wild Russula griseocarnosa, where increasing the extraction temperature from 50 to 90 °C and extraction time from 1 to 5 h increased extraction yield of said polysaccharides. According to the crude protein from grapeseed, extraction temperature had a positive impact on protein yield, while extraction time did not affect the protein yield [29]. However, results for optimal extraction temperature and extraction time were found in the other plant species and compounds. Elsewhere, the extraction temperature increased the extraction rate of soluble soybean polysaccharides, but extraction time had an optimal value range: increasing the extraction time from 2 to 4 h increased the polysaccharides, while any additional increase in extraction time from 4 to 6 h decreased the polysaccharides [30]. Hou et al. [31] optimized the extraction condition of phenolic and flavonoid compounds from Melaleuca bracteata leaves and revealed that increasing the extraction temperature increased total phenolic content. Increasing the extraction time from 1 to 3 days led to an increased total phenolic content while increasing the extraction time from 3 to 5 days led to a decrease in the total phenolic content as well as the total flavonoid content. According to the total flavonoid content, the extraction temperature has a small effect. Ni et al. [32] optimized the decoction condition of baicalin from Scutellaria baicalensis roots. Here, the extraction time was varied from 30 to 150 min. It was found that by increasing the extraction time from 30 to 60 min, baicalin content was increased, while when increasing the extraction time from 60 to 150 min, the baicalin content was decreased. The highest baicalin content was achieved at an extraction time of 60 min. Other works reported that extraction time also showed a positive impact on the flavonoid content of Passiflora quadrangularis leaves obtained using the percolation technique; total flavonoids content was increased when extraction time was increased from 24 to 72 h [33]. Uddin et al. [34] optimized the extraction of polysaccharides from Pleurotus ostreatus. These authors showed that the extraction temperature and extraction time had optimum values of 70 °C and 60 min, respectively, as shifting higher or lower from these optimum values decreased the polysaccharide yield. Furthermore, an optimal extraction temperature and extraction time were observed in the extraction of polysaccharides from Eucommia ulmoides leaves [35].
Our previous works demonstrated the extraction of plant active compounds from cannabis [36], Centella asiatica [37], and Vernonia cinerea [38]. All works applied circumscribed central composite experimental design, which has been widely used in response to surface modeling and optimization [39]. The above works established the extraction of nonvolatile compounds, i.e., cannabinoids, centelloids, and nitrate, respectively. However, they were different in detail. Extraction of nitrate from V. cinerea used water as a solvent. An explosion or ignition during the extraction process could be negligible. Thus, the high applied temperature or boiling could be used, so the high temperature up to 100 °C for 10-60 min was designed [38]. According to C. asiatica and cannabis, the active compounds were soluble in ethanol, so ethanol was selected as a safe extraction solvent. Due to the lower flash point and boiling point of ethanol compared with water, the lower extraction temperature was used: 40-60 °C for C. asiatica [37] and 40-80 °C for cannabis [36], and the longer extraction time could be applied: 60-120 min for C. asiatica [37] and 30-90 min for cannabis [36]. Our present work also used ethanol to extract trans-anethole, a volatile substance, so the low temperature range, 40-80 °C, and the short duration time, 10-30 min were selected. The inclusion of the factors into the experimental design affected the overall result. The comparable experimental design for extraction of active compounds from different plants could be different, due to the different plant components, physicochemical properties of active compounds, the interaction of chemical compounds in plant matrix, etc. This section could be summarized that the optimization of the extraction process was an important step to maximize plant bioactive compounds as well as their activities, especially the extraction of volatile compounds when heating was applied.
Our previous work demonstrated that extraction method, defatting, and solvent-to-solid ratio affected nitrate content of C. anisata leaves. Infusion of a non-defatted sample using a solvent-to-solid ratio of 10:1 provided the highest nitrate content of the raw material, while decoction of a defatted sample gave the highest nitrate content. Moreover, increasing the solvent-to-solid ratio and defatting of the decoction sample had a huge effect on the nitrate content [17]. Our other previous works optimized the dynamic maceration of C. asiatica. Extraction temperature and extraction time were varied to investigate their effects on the content of four markers, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Madecassoside and asiaticoside were highly extracted using a long extraction time and high extraction temperature, while madecassic acid and asiatic acid were highly extracted at both a high extraction temperature and long extraction time as well as at a low extraction temperature and short extraction time [37]. Other works by our colleagues investigated the effects of the same independent factors, i.e., extraction temperature and extraction time on some responses such as extraction yield and brazilin content. Extraction temperature showed a positive effect on the extraction yield and brazilin content of Caesalpinia sappan heartwood, while extraction time had a minor effect [40]. This outcome was opposite to our present work, where extraction time had a positive effect on extraction yield, but the extraction temperature had a minor effect. Furthermore, other previous works evaluated the effects of extraction temperature and extraction time on the nitrate content of V. cinerea. The result showed that both extraction temperature and extraction time have a positive effect on extraction yield and nitrate content. This result was associated with a thermostable property of nitrate, so increasing the temperature and duration time extracted the high content nitrate from V. cinerea [38]. All the above data indicated that responsiveness to extraction temperature and extraction time was dependent on the plant species and plant compounds involved.

Conclusions
This work sought to optimize the dynamic maceration of C. anisata leaves to maximize the trans-anethole content. Trans-anethole content was determined by a linear response, specific, precise, and accurate HPLC method. Extraction time had a positive effect on extraction yield, while extraction temperature had a minor effect. Transanethole content in the extract, as well as in plant raw material, was maximized when a medium extraction temperature with a short extraction time was used. The optimal conditions that maximized trans-anethole content were an extraction temperature of 61.8 °C and an extraction time of 12.9 min. Furthermore, the prediction by Design-Expert ® was accurate. The condition of dynamic maceration will be used as a guide for the extraction of trans-anethole from C. anisata leaves to use as an ingredient of food, beverage, or herbal products.

Conflict of interest
The authors declare that they have no conflicts of interest. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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